Adam17 (NM_020306) Rat Tagged ORF Clone

CAT#: RR214103

Adam17 (Myc-DDK-tagged ORF) - Rat ADAM metallopeptidase domain 17 (Adam17), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam17
• Synonyms: TACE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214103 representing NM_020306
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCAGCGTCTCCTCTTCCTGACCACTTTGGTGCCTTTCGTCCTGGCACCCCGACCTCCGGAGGAAC
  CAGGCTCTGGCTCCCACCTGCGACTTGAGAAGCTTGATTCTTTGCTCTCAGACTACGATATCCTCTCTTT
  ATCTAATATCCAGCAGCACTCCATAAGGAAAAGGGATCTACAGTCTGCGACACACTTAGAAACACTACTA
  ACTTTTTCAGCTTTGAAAAGGCATTTTAAATTATACTTGACATCAAGTACAGAACGCTTTTCACAGAACT
  TGCGAGTCGTGGTGGTGGATGGGAAAGAGGAAAGCGAGTACAGTGTGAAGTGGCAGGACTTCTTCAGCGG
  ACATGTGGTTGGTGAGCCTGACTCTAGGGTTCTAGCGCACATAGGAGATGATGATGTCACAGTAAGAATC
  AACACAGATGGGGCAGAATATAACATAGAGCCACTTTGGAGGTTTGTTAATGATACTAAAGATAAAAGGA
  TGCTGGTGTATAAGTCTGAAGATATCAAGGATTTTTCACGTTTGCAGTCTCCAAAAGTATGTGGTTATTT
  AAATGCAGATAGTGAAGAGTTGCTTCCAAAAGGGCTCATAGACAGAGAGCCATCTGAAGAGTTTGTCCGT
  CGAGTGAAGAGGCGAGCTGAACCTAACCCATTGAAGAATACTTGTAAATTACTGGTGGTGGCAGATCATC
  GATTTTATAAGTACATGGGCCGAGGAGAAGAGAGCACTACTACAAATTACTTAATAGAGTTAATTGACCG
  AGTTGATGACATATACCGGAACACCTCGTGGGACAATGCAGGATTTAAAGGTTATGGAGTACAGATAGAA
  CAGATTCGAATTCTCAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTT
  TTCCAAATGAAGAAAAGGATGCTTGGGATGTGAAGATGCTGCTGGAGCAATTTAGCCTTGATATAGCTGA
  AGAGGCCTCTAAAGTCTGCCTGGCTCATCTTTTCACCTACCAAGATTTTGATATGGGAACTCTTGGATTA
  GCTTACGTTGGTTCTCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAGGTG
  TGAAGAAGAACATCTATTTGAATAGTGGTCTGACAAGTACAAAAAATTATGGTAAAACCATCCTTACAAA
  GGAGGCTGACCTGGTTACAACTCATGAATTGGGACACAATTTTGGAGCAGAACATGATCCTGATGGGCTG
  GCAGAGTGTGCCCCAAATGAGGACCAAGGAGGAAAGTATGTTATGTACCCCATAGCTGTGAGTGGTGACC
  ATGAGAATAATAAGATGTTTTCAAACTGCAGTAAACAGTCCATCTACAAGACCATAGAAAGCAAGGCTCA
  AGAGTGCTTCCAGGAGCGCAGCAACAAGGTGTGCGGCAACTCCAGGGTGGACGAAGGAGAAGAGTGTGAC
  CCGGGCATCATGTACCTGAACAACGACACCTGCTGCAATAGTGACTGCACACTGAAGCCAGGTGTGCAGT
  GCAGTGATAGGAATAGTCCTTGCTGTAAAAATTGTCAGTTTGAGACGGCCCAGAAGAAGTGCCAGGAGGC
  TATCAATGCTACTTGCAAAGGAGTGTCTTACTGCACAGGGAATAGCAGTGAGTGCCCCCCACCAGGAGAT
  GCTGAAGATGACACTGTGTGCTTGGACCTGGGCAAGTGCAAGGCTGGGAAATGCATCCCTTTCTGCAAGA
  GGGAGCAGGAGCTGGAGTCCTGCGCATGTGCTGACACCGACAACTCGTGCAAGGTATGCTGCAGGAACCT
  TTCTGGCCCATGTGTGCCTTACGTCGATGCAGAGCAAAAGAACTTATTTTTGAGGAAAGGGAAGCCCTGT
  ACAGTAGGGTTTTGTGACATGAATGGCAAATGTGAGAAACGGGTACAGGACGTAATTGAGCGGTTTTGGG
  ATTTCATTGACCAGCTGAGCATCAACACTTTTGGGAAGTTTCTGGCAGACAACATCGTTGGGTCTGTTCT
  GGTTTTCTCCTTGATATTTTGGATTCCTTTCAGCATTCTTGTCCACTGTGTGGATAAGAAACTGGACAAG
  CAGTATGAGTCCCTGTCTCTGTTTCATCACAGTAACATTGAGATGTTGAGCAGCATGGACTCAGCATCTG
  TTCGCATTATCAAGCCGTTCCCTGCACCCCAGACTCCAGGTCGTCTGCAGGCCCTGCAGCCGGCTGCAAT
  GATGCCGCCAGTGTCTGCGGCTCCAAAACTGGATCACCAGAGGATGGACACCATCCAGGAAGACCCCAGC
  ACAGATTCACATGTGGATGATGATGGCTTTGAGAAGGACCCCTTCCCCAACAGCAGTGCAGCCGCCAAGT
  CCTTTGAGGACCTCACAGACCACCCAGTCACCAGGAGTGAGAAGGCCGCCTCCTTCAAGCTGCAGCGTCA
  GAGCCGAGTTGATAGCAAAGAGACAGAGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214103 representing NM_020306
  Red=Cloning site Green=Tags(s)
  
  MRQRLLFLTTLVPFVLAPRPPEEPGSGSHLRLEKLDSLLSDYDILSLSNIQQHSIRKRDLQSATHLETLL
  TFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQDFFSGHVVGEPDSRVLAHIGDDDVTVRI
  NTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVR
  RVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIE
  QIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSLDIAEEASKVCLAHLFTYQDFDMGTLGL
  AYVGSPRANSHGGVCPKAYYNPGVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGL
  AECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECD
  PGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGD
  AEDDTVCLDLGKCKAGKCIPFCKREQELESCACADTDNSCKVCCRNLSGPCVPYVDAEQKNLFLRKGKPC
  TVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDK
  QYESLSLFHHSNIEMLSSMDSASVRIIKPFPAPQTPGRLQALQPAAMMPPVSAAPKLDHQRMDTIQEDPS
  TDSHVDDDGFEKDPFPNSSAAAKSFEDLTDHPVTRSEKAASFKLQRQSRVDSKETEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020306
• ORF Size: 2481 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020306.1, NM_020306.2, NP_064702.1
• RefSeq Size: 4126
• RefSeq ORF: 2484
• Locus ID: 57027
• MW: 93 kDa
• Gene Summary: expression occurs in response to oxygen glucose deprivation, may play a role in inhibition of apoptosis via induction of TNF-alpha release [RGD, Feb 2006]