Ruvbl1 (NM_147177) Rat Tagged ORF Clone

CAT#: RR214117

Ruvbl1 (Myc-DDK-tagged ORF) - Rat RuvB-like 1 (E. coli) (Ruvbl1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ruvbl1
• Synonyms: NMP238; Pontin52; Tip49a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214117 representing NM_147177
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTGAGGAGGTGAAGAGCACCACGAAGACGCAACGCATTGCCTCCCACAGCCACGTGAAGGGGC
  TGGGGCTGGATGAGAGCGGCCTGGCCAAGCAGGCGGCTTCGGGGCTCGTGGGCCAGGAGAACGCGAGAGA
  GGCATGTGGTGTCATAGTCGAATTAATCAAAAGCAAGAAAATGGCTGGAAGAGCTGTCTTGTTGGCAGGG
  CCTCCTGGAACTGGCAAGACAGCCTTGGCCCTGGCTATTGCTCAGGAACTGGGCAGTAAAGTCCCTTTCT
  GCCCGATGGTGGGTAGTGAAGTATACTCAACTGAGATCAAGAAGACAGAGGTGCTGATGGAGAACTTCCG
  AAGGGCTATTGGGCTGCGGATAAAGGAGACTAAGGAGGTTTATGAAGGGGAGGTGACAGAGCTCACTCCC
  TGTGAGACAGAGAACCCCATGGGTGGGTATGGCAAAACTATCAGCCACGTGATCATAGGGCTCAAGACTG
  CCAAAGGAACCAAACAGCTGAAGCTGGACCCCAGTATTTTTGAAAGTTTGCAGAAAGAACGAGTAGAGGC
  TGGAGATGTGATTTACATTGAAGCAAACAGTGGAGCTGTGAAGAGGCAAGGCAGGTGTGACACCTATGCC
  ACAGAGTTTGACCTTGAGGCTGAAGAGTATGTCCCTTTGCCAAAGGGAGATGTGCACAAGAAGAAGGAAA
  TCATACAGGATGTGACCTTGCACGACTTGGACGTGGCCAATGCGCGGCCTCAGGGTGGGCAAGATATTCT
  GTCTATGATGGGCCAGTTGATGAAGCCAAAAAAGACAGAGATCACAGATAAACTTCGAGGGGAGATCAAC
  AAGGTGGTGAACAAATACATTGACCAGGGCGTTGCAGAGCTGGTCCCTGGAGTGCTCTTTGTTGACGAGG
  TCCACATGCTGGATATCGAGTGCTTTACCTACCTGCACCGAGCCCTGGAGTCCTCCATCGCCCCCATTGT
  CATCTTTGCATCCAACCGAGGCAACTGTGTCATCAGGGGCACCGAGGACATCACTTCTCCACACGGCATC
  CCGTTGGACCTGCTGGACCGGGTGATGATCATCAGGACCATGCTGTATACGCCACAGGAGATGAAGCAGA
  TCATTAAGATCCGAGCCCAGACGGAAGGCATCAACATCAGTGAGGAGGCCCTAAACCACCTCGGGGAGAT
  TGGCACCAAGACCACGCTGAGGTATTCAGTGCAGCTGCTGACCCCTGCCAACCTGCTGGCCAAGATCAAC
  GGGAAGGACAGCATTGAGAAGGAGCACGTGGAGGAGATCAGCGAGCTCTTCTATGACGCCAAGTCCTCCG
  CCAAGATTCTGGCCGACCAGCAGGACAAGTACATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214117 representing NM_147177
  Red=Cloning site Green=Tags(s)
  
  MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAG
  PPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTP
  CETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYA
  TEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEIN
  KVVNKYIDQGVAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGI
  PLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKIN
  GKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_147177
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_147177.1, NP_671706.1
• RefSeq Size: 1587
• RefSeq ORF: 1371
• Locus ID: 65137
• MW: 50.2 kDa
• Gene Summary: nuclear DNA helicase that binds TBP; involved in recombination and transcription [RGD, Feb 2006]