Pi4ka (NM_022301) Rat Tagged ORF Clone

CAT#: RR214160

Pi4ka (Myc-DDK-tagged ORF) - Rat phosphatidylinositol 4-kinase, catalytic, alpha (Pi4ka), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pi4ka
• Synonyms: Pik4ca
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214160 representing NM_022301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCCAGTAGATTTTCATGGAATCTTCCAGCTAGATGAAAGACGGAGAGATGCAGTGATTGCTCTGG
  GCATTTTTCTCATTGAGTCTGATCTTCAGCACAAAGATTGTATTGTTCCTTATCTTCTTCGACTTCTCAG
  GGGTCTTCCAAAAGTCTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGGTAACCTCCCGGTTGCA
  GAAAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCGCTTAGGGATG
  AGATTTTAGAGGCACTTTTGCAGGTTTTACATGTCCTTCTGGGGATGTGCCAGGCCCTGGAGATTCAAGA
  GAAAGAATACCTTTGCAAGTATGCAATCCCATGCCTGATAGGGATCTCGAAATCTTTTGGACGTTACAGC
  AATTCAGAAGAATCTCTCCTCTCAAAGCTTTTTCCCAAAGTCCCTCCTCATTCCCTGAGAATCCCCGAAG
  AGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTCCGCTCCATCCTGCCCAGCAACCTGCTGACTGT
  CTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGT
  GGCATGCCCCCTCCCAGCTCCCCTGGAGGATCTGCTTTTCACTATTTTGAAGCCTCTTGCCTGCCTGATG
  GAACCACCTTGGAGCCCGAATACTACTTCTCTACCATCAGCTCCAGTTTTTCTGTCTCTCCCCTGTTCAA
  TGGTATCACCTATAAGGAGTTCTATATTCCACTGGAAATGCTTCGAGAACTCTTAAACTTGGTAAAGAAG
  ATTGTTGAGGAGCCTGTTCTCAAGTCCTTAGATGCAGCTGTAGCCAGAGTGATGGAGGCCAATCCCAGTG
  CTGACCTATACTACACGACCTTCAGTGATCCCGTCTACCTGACCATGTTCAAGATGCTTCGAGACACTCT
  GTACTATATGAAAGACTTACCCACCTCCTTTGTGAAAGAGATCCATGATTTTGTGTTGGAGCAGTTCAAC
  ATGAGCCAAGGGGAACTCCAAAAGATTCTCCATGATGCAGACAGGATCCACAGTGAGATGAGCCCACTCA
  AACTGCGCTGCCAAGCAAATGCTGCCTGCGTAGACCTCATGGTGTGGGCTGTGAAGGATGAGCAGGGTGC
  AGAAAACCTTTGCATCAAGCTGTCTGAAAAGCTGCAGTCCAAGACATCCAGCAAGGTCATCATCGCCCAT
  TTGCCCTTGCTGATCTGCTGCCTACAGGGTCTGGGCCGCCTATGTGAACGGTTCCCGGTGGTGGTCCACT
  CAGTGACACCATCTTTGAGAGACTTTCTGGTGATTCCATCCCCAGTGCTGGTGAAGCTCTATAAGTACCA
  CAGTCAGTACCACACAGTTGCTGGCAGTGATATAAAAATTAGTGTCACCAATGAGCATTCTGAGTCAACT
  CTGAATGTCTTGCCCGGCAAGAAGAACCAGCCATCCATGTATGAGCAGCTCCGGGACATCGCCATTGACA
  ACATCTGCAGGTGCCTGAAGGCCGGCCTCACCGTGGACCCTGTGATCGTGGAGGCCTTCCTGGCCAGCCT
  CTCCAACCGGCTCTATATCTCCCAGGAGAGCGATAAGGATGCTCACTTGATTCCTGACCATACCATTCGT
  GCCTTGGGACACATTGCAGTGGCTCTGAGGGATACCCCAAAGGTCATGGAGCCAATTCTGCAGCAGAAAT
  TTTGCCAGCCCCCCTCACCCCTCGATGTGCTCATCATCGACCAGCTGGGCTGCCTGGTTATCACTGGAAA
  TCAATACATTTATCAGGAAGTGTGGAATCTCTTCCAACAAATCAGTGTGAAGGCCAGTTCAGTTGTATAT
  TCAGCCACCAAAGACTACAAAGACCATGGCTACAGGCACTGCTCCCTCGCAGTCATCAATGCCCTGGCCA
  ACATCGCAGCCAACATTCAGGAGGAGCACCTTGTGGATGAGCTGTTGATGAACCTGCTGGAACTGTTTGT
  GCAGCTGGGGCTGGAGGGGAAGAGAGCCAGTGAGAGGGCCAGCGAGAAGGGGCCCGCCCTGAAGGCTTCC
  AGCAGTGCAGGGAATTTGGGAGTGCTCATTCCTGTAATAGCCGTGCTCACCCGACGATTGCCACCCATCA
  AAGAAGCTAAACCTAGGCTACAAAAGCTTTTCCGAGACTTCTGGCTCTACTCTGTGCTAATGGGATTCGC
  TGTGGAAGGCTCAGGGCTCTGGCCGGAAGAGTGGTATGAAGGAGTCTGTGAAATAGCAACCAAGTCACCT
  CTGCTTACCTTTCCCAGCAAAGAGCCTCTTCGATCAGTCCTCCAGTACAACTCAGCCATGAAAAATGATA
  CAGTGACCCCGGCTGAGCTGAATGAGCTCCGAAGTACCATCATCAACTTGCTAGATCCTCCTCCTGAGGT
  GTCTGCCCTCATCAACAAGCTGGACTTTGCCATGTCCACCTATCTCTTGTCTGTGTATCGGCTGGAATAC
  ATGAGGGTGCTGCGTTCTACAGATCCAGATCGCTTCCAGGTAATGTTCTGCTACTTCGAGGATAAAGCTA
  TTCAGAAAGACAAATCTGGGATGATGCAGTGTGTCATCGCTGTTGCTGACAAAGTATTTGATGCCTTCCT
  GAACATGATGGCAGAAAAAGCCAAGACCAAGGAGAATGAAGAGGAGTTGGAGCGGCATGCCCAGTTTCTG
  CTGGTGAACTTCAACCACATTCACAAGAGGATACGCAGAGTGGCTGACAAGTACCTGTCTGGTCTGGTGG
  ATAAGTTTCCACACTTGCTCTGGAGTGGGACTGTGCTGAAGACCATGTTGGACATCCTGCAGACCCTGTC
  ACTATCACTAAGTGCTGATATTCACAAGGACCAACCTTATTATGACATTCCTGACGCCCCCTATCGGATC
  ACGGTTCCTGACACGTATGAAGCCCGGGAGAGCATCGTGAAAGACTTTGCTGCACGCTGTGGGATGATCC
  TGCAGGAAGCCATGAAGTGGGCACCCACGGTCACTAAGTCCCACCTGCAGGAATATCTGAACAAGCATCA
  GAACTGGGTGTCAGGACTGTCCCAGCACACGGGATTGGCCATGGCCACTGAGAGCATCCTTCACTTTGCC
  GGCTACAACAAGCAGAACACAACTCTTGGGGTAACTCAGCTCACTGAGCGCCCGGCCTGTGTGAAGAAAG
  ACTACTCCAACTTCATGGCATCCTTGAATTTGCGCAACCGCTATGCCGGCGAGGTGCACGGGATGATTCG
  GTTCTCAGGTGCCACAGGCCAGATGTCCGACCTGAACAAAATGATGGTCCAGGATCTGATTACAGCTCTG
  GACCACAGTCATCCTCAGCACTATACCCAAGCCATGTTCAAATTGACGGCCATGCTCATTAGCAGCAAAG
  ATTGTGACCCACAGCTCCTTCATCATCTGTGTTGGGGTCCCCTCCGGATGTTCAACGAGCATGGCATGGA
  GACTGCACTAGCCTGCTGGGAGTGGCTGCTGGCTGGCAAGAATGGAGTGGAAGTGCCGTTCATGCGGGAG
  ATGGCAGGAGCCTGGCACATGACAGTGGAGCAGAAGTTCGGCCTCTTCTCTGCAGAAACAAAGGAAGCAG
  ACCCCCTGGCAGCGTCAGAAGCAAGTCAGCCACGACCCTGTCCCCCTGAAGTGACACCTCACTACATCTG
  GATTGATTTCCTGGTGCAGCGGTTTGAGATTGCCAAGTACTGTAGCTCTGACCAGGTGGAGATCTTCTCA
  AGCCTGCTGCAGCGCTCCATGTCCCTCCACATCGGTGGAGCCAGGGGGAGCATGAACAGGCATGTGGCAG
  CCATTGGACCACGTTTCAAACTGCTGACCCTGGGGCTGTCCCTCCTACACGCTGATGTGGTTCCAAATGC
  AACCATTCGAAACGTGCTTCGAGAGAAGATCTACTCAACAGCCTTTGACTATTTCAGTTGTCCACCAAAG
  TTCCCTACTCAAGGAGAAAAGAGGCTGCGTGAAGATATAAGCATCATGATTAAGTTTTGGACTGCCATGT
  TCTCAGATAAGAAGTACCTGACTGCCAGCCAACTCGTTCCCCCAGATAACCAGGACACCCGAAGCAACTT
  GGATATAACTGTGGGCTCTCGACAACAGGCTACACAGGGATGGATCAACACATACCCCCTCTCCAGTGGC
  ATGTCAACTATCTCCAAAAAATCAGGCATGTCTAAGAAAACCAACCGAGGTTCCCAGCTGCACAAGTACT
  ACATGAAGCGTAGGACGCTGCTGCTGTCACTGTTGGCCACTGAGATCGAGCGCCTCATCACTTGGTACAA
  CCCGCTGTCAGCCCCAGAGCTAGAGCTGGACCAGGCAGGTGAGAACAGTGTTGCCAACTGGAGGTCCAAG
  TATATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTTGCCTGGACCATCTCTCCATACCTCG
  CTGTGCAGCTGCCAGCCAGGTTCAAGAATACAGAAGCCATTGGAAATGAAGTGACCCGTCTCGTTCGCCT
  GGACCCAGGAGCTGTTAGTGATGTCCCTGAAGCTATCAAGTTCCTTGTCACCTGGCACACCATCGATGCT
  GATGCTCCAGAGCTTAGCCATGTGTTATGCTGGGCACCCACGGACCCGCCCACGGGCCTCTCCTACTTCT
  CCAGCATGTACCCCCCACACCCTCTCACTGCACAGTATGGGGTGAAAGTCCTGCGGTCCTTCCCTCCGGA
  TGCCATTCTGTTCTACATCCCCCAGATTGTGCAGGCCCTCAGATATGACAAGATGGGCTATGTGCGCGAG
  TACATTCTCTGGGCTGCAGCCAAATCCCAGCTTCTGGCACACCAGTTTATTTGGAATATGAAAACTAACA
  TCTACCTTGATGAAGAAGGGCACCAGAAAGACCCTGACATTGGTGACCTTCTGGAGCAGTTAGTAGAAGA
  AATCACAGGGTCTCTATCAGGCCCAGCCAAAGATTTCTATCAACGGGAATTTGATTTCTTTAACAAGATC
  ACCAACGTGTCAGCTATCATCAAGCCCTACCCTAAAGGCGATGAAAGAAAGAAGGCTTGTCTGTCAGCCC
  TGTCTGAAGTGAAGGTGCAGCCTGGCTGCTACCTGCCCAGCAACCCTGAAGCAATTGTGCTAGACATTGA
  CTACAAGTCAGGGACACCAATGCAGAGTGCTGCAAAAGCGCCGTATCTTGCCAAGTTTAAGGTGAAGCGA
  TGCGGAGTGAGTGAACTTGAAAAAGAAGGTCTGCAGTGCCGCTCAGACACAGAGGATGAGTGCCGCAGGC
  AAGAGGCTGATGGCAAGAAGATCTGCTGGCAAGCAGCCATCTTCAAAGTGGGGGATGACTGCCGACAGGA
  CATGCTAGCCCTGCAGATCATCGACCTTTTCAAGAACATCTTCCAGCTGGTTGGCCTGGACCTCTTCGTT
  TTTCCCTATCGGGTAGTGGCTACTGCCCCTGGGTGTGGGGTGATTGAGTGCATTCCAGACTGTACCTCCC
  GAGACCAGCTAGGCCGCCAGACTGACTTCGGCATGTATGACTACTTTACACGCCAGTATGGGGACGAGTC
  TACCTTGGCCTTCCAGCAGGCACGCTACAACTTTATCAGAAGCATGGCGGCCTACAGCCTCCTGCTGTTC
  CTGCTGCAGATCAAGGACAGGCACAACGGCAACATCATGCTGGACAAGAAGGGACACATCATCCACATAG
  ACTTTGGCTTCATGTTTGAAAGCTCACCGGGTGGCAACCTTGGCTGGGAGCCAGACATCAAGCTGACAGA
  CGAGATGGTGATGATTATGGGGGGCAAGATGGAAGCCACACCCTTCAAGTGGTTCATGGAGATGTGCGTC
  CGAGGTTATCTGGCTGTGCGCCCCTATATGGATGCAGTCGTCTCCCTGGTTACACTCATGTTGGACACAG
  GCCTGCCCTGTTTCCGTGGCCAGACAATCAAACTTTTGAAACACAGGTTCAGCCCGAACATGACAGAGCG
  GGAGGCTGCAAATTTTATCATGAAGATCATCCAAAACTGTTTCCTCAGCAACAGGAGCCGGACCTACGAC
  ATGATCCAGTACTACCAGAATGATATCCCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214160 representing NM_022301
  Red=Cloning site Green=Tags(s)
  
  MCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCIVPYLLRLLRGLPKVYWVEESTARKGRGNLPVA
  ESFSFCLVTLLSDVACRDPSLRDEILEALLQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISKSFGRYS
  NSEESLLSKLFPKVPPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPER
  GMPPPSSPGGSAFHYFEASCLPDGTTLEPEYYFSTISSSFSVSPLFNGITYKEFYIPLEMLRELLNLVKK
  IVEEPVLKSLDAAVARVMEANPSADLYYTTFSDPVYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFN
  MSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAH
  LPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSEST
  LNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIR
  ALGHIAVALRDTPKVMEPILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVY
  SATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKAS
  SSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSP
  LLTFPSKEPLRSVLQYNSAMKNDTVTPAELNELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEY
  MRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFL
  LVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRI
  TVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFA
  GYNKQNTTLGVTQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITAL
  DHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMRE
  MAGAWHMTVEQKFGLFSAETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFS
  SLLQRSMSLHIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPK
  FPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSG
  MSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSK
  YISLSEKQWKDNVNLAWTISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDA
  DAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVRE
  YILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKI
  TNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKR
  CGVSELEKEGLQCRSDTEDECRRQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFV
  FPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLF
  LLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCV
  RGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKIIQNCFLSNRSRTYD
  MIQYYQNDIPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022301
• ORF Size: 6123 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022301.1, NP_071637.1
• RefSeq Size: 6579
• RefSeq ORF: 6126
• Locus ID: 64161
• MW: 231.3 kDa
• Gene Summary: catalyzes the phosphorylation of phosphatidylinositol (PI) at the D-4 position of inositol ring [RGD, Feb 2006]