Hip1 (NM_001100475) Rat Tagged ORF Clone

CAT#: RR214181

Hip1 (Myc-DDK-tagged ORF) - Rat huntingtin interacting protein 1 (Hip1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214181 representing NM_001100475
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGGATGGCCAGCTCCATGAAGCAGGTACCCAACCCGCTGCCCAAAGTGTTGAGCCGGCGCGGGG
  TTGGTGCTGGGATGGAGGCTGCGGAGCGCGAGAGCTTCGAGCGGACTCAGACGGTCAGCGTCAATAAGGC
  CATTAATACGCAGGAAGTGGCCGTGAAGGAAAAACATGCCAGGACGTGTATTCTGGGCACCCACCATGAG
  AAAGGGGCGCAGACCTTCTGGTCTGTGGTCAACCGACTGCCTCTCTCCAGCAACGCCATGCTGTGCTGGA
  AGTTCTGCCACGTGTTCCACAAACTCCTTCGAGACGGACATCCGAACGTGCTGAAAGACTCTCTCAGATA
  CAAAAATGAGTTAAGCGACATGAGCAGGATGTGGGGCCACCTGAGTGAGGGGTATGGACAGCTCTGCAGC
  ATCTACCTCAAACTGCTGAGAACAAGGATGGAGTACCACACCAAAAATCCCAGGTTCCCAGGTAACCTCC
  AGATGAGCGACCGGCAGCTGGATGAAGCTGGGGAAAGTGATGTTAACAACTTTTTCCAGCTCACAGTGGA
  GATGTTTGATTACCTGGAGTGCGAACTGAACCTCTTTCAAACTGTGTTCAACTCCTTGGACATGTCCCGC
  TCAGTGTCCGTGACCACAGCTGGCCAGTGCCGCCTCGCCCCGCTCATCCAGGTCATCCTGGACTGCAGTC
  ACCTCTATGACTACACTGTCAAGCTGCTCTTCAAGCTCCACTCCTGTCTTCCAGCTGACACCCTGCAGGG
  CCACCGAGACCGCTTCATGGAGCAGTTCACAAAGTTGAAAGATCTGTTTCAGCGCTCCAGCAACCTTCAG
  TACTTCAAGCGGCTCATTCAGATCCCCCAGTTGCCTGAGAATCCACCCAACTTCCTACGAGCCTCGGCCC
  TGTCCGAGCACATCAGTCCTGTGGTGGTGATCCCGGCTGAGGTGTCGTCTCCAGACAGTGAGCCCGTCCT
  GGAGAAGGATGACCTCATGGACATGGATGCCTCCCAGCAGAGTTTGTTTGACAACAAGTTTGATGACATC
  TTCGGCAGCTCGTTGAGCAGTGACCCCTTCAATTTCAACAATCAAAATGGCGTGAACAAGGATGAGAAGG
  ACCACTTGATTGAACGCCTCTACAGAGAGATCAGTGGACTGACAGGACAGCTGGACAACGTGAAGATTGA
  GAGCCAGCGGGCCATGCTGCAGCTGAAGGGTCGCGTGAGTGAGCTGGAAGCAGAGCTGGCCGAGCAGCAG
  CACCTGGGACGGCAGGCTACGGATGACTGCGAGTTCCTGCGCACGGAGCTGGATGAACTGAAGAGGCAGC
  GAGAGGACACTGAGAAGGCGCAGCGCAGCCTGACTGAGATAGAAAAAAAGGCCCAGGCCAATGAACAGCG
  GTATAGCAAGTTAAAAGAGAAGTACAGTGAACTGGTGCAGAACCATGCTGATCTGCTGCGGAAGAATGCA
  GAGGTGACCAAGCAGGTGTCTGTGGCCCGGCAAGCCCAGGTGGATTTGGAAAGAGAGAAAAAAGAACTAG
  CGGATTCCTTTGCACGTACTCAAGAGCAACAGGATGTTCTAGAGACCCTGAAGCACGAACTGGCCACCAG
  CAGGCAAGAGCTGCAGGTCCTCCATGGCAACCTGGAAACCTCTGCCCAGTCTGAGGCGAAATGGCTAACG
  CAGATCGCGGAGCTGGAGAAGGAACAAGGCAGTTTGGCGACAGCCGCCGCTCAAAGAGAGGAAGAGTTCT
  CAGCCCTCCGAGACCAACTGGAAAGCACCCAGCTCAAACTGGCTGGTGCCCAGGATTCCATGTGTCAGCA
  GATAAAGGACCAGAGGAGAATCCTCTTGGCAGGGGCCAGGAAGGCTGCGGAGCTCGAGATACAGGAGGCC
  CTGGGCCAGCTTGAAGAGCCTGCCCTCCTCATCAGCTGTGCAGGAGCCACAGATCATCTTCTCTCCAAAG
  TCAACTCCGTTTCCAGCTGCCTCGAGCAACTGGAAAAGAGCCGCAGCCAGTATCTGGCCTGCCCAGAAGA
  GATCAGTGAGCTTCTGCACTCAATAACCCTGCTTGGCCACTTGACCAGTGACACCATCATTCAGGGGACT
  GCCACCAGCCTCCGGGCCCCACCAGAGTCAGCCGACTCTCTGACGGAGGCCTGTAGGCAGTATGGCAGAG
  AAACTCTGGCCTATCTGTCCTCCCTAGAGGAAGAGGGGGCCATGGAAAAAGCTGACACCACAGCCATAGC
  GAACTGCCTCGGCAAGATCAAGACCATTGGCGAGGAACTGCTGCCCAGGGGCTTGGACATCAAGCAGGAA
  GAGCTGGGTGACCTGGTGGACAAGGAGATGGCAGCCACTTCAGCTGCCATTGAAGCTGCCACCACCCGGA
  TAGAGGAAATTCTCAGTAAGTCCCGAGCAGGAGACACGGGAGTCAAGTTGGAGGTGAACGAGAGGATCCT
  TGGCTCCTGTACCAGCCTGATGCAAGCTATCAAGGTGCTCGTTGTGGCCTCCAAGGACCTCCAGAAGGAG
  ATAGTGGAAAGCGGCAGGGGTACCGCATCCCCTAAAGAATTTTACGCCAAGAACTCTCGGTGGACAGAAG
  GGCTCATATCTGCCTCCAAAGCTGTTGGTTGGGGAGCTACCATCATGGTGGATGCTGCTGACCTTGTGGT
  CCAAGGCAAAGGGAAGTTCGAGGAGCTGATGGTGTGCTCGCATGAGATTGCCGCTAGCACAGCCCAGCTT
  GTGGCTGCATCCAAGGTGAAAGCGAACAAGGGCAGCCTCAATCTGACCCAGCTGCAGCAGGCCTCTCGGG
  GAGTGAACCAGGCCACGGCCGCTGTGGTGGCCTCAACCATTTCCGGCAAATCTCAAATTGAGGAAACAGA
  CAGTATGGACTTCTCAAGCATGACACTGACCCAGATCAAGCGCCAGGAAATGGATTCCCAGGTTAGGGTG
  CTGGAGCTAGAAAACGACCTGCAGAAGGAGCGCCAGAAACTAGGAGAGCTTCGGAAAAAACACTACGAGC
  TGGCTGGTGTTGCTGAGGGCTGGGAAGAAGGGACAGAAGCATCACCTTCTGCTGTTCAAGAAGCGGTGCC
  GGACAAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214181 representing NM_001100475
  Red=Cloning site Green=Tags(s)
  
  MDRMASSMKQVPNPLPKVLSRRGVGAGMEAAERESFERTQTVSVNKAINTQEVAVKEKHARTCILGTHHE
  KGAQTFWSVVNRLPLSSNAMLCWKFCHVFHKLLRDGHPNVLKDSLRYKNELSDMSRMWGHLSEGYGQLCS
  IYLKLLRTRMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSR
  SVSVTTAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFQRSSNLQ
  YFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEVSSPDSEPVLEKDDLMDMDASQQSLFDNKFDDI
  FGSSLSSDPFNFNNQNGVNKDEKDHLIERLYREISGLTGQLDNVKIESQRAMLQLKGRVSELEAELAEQQ
  HLGRQATDDCEFLRTELDELKRQREDTEKAQRSLTEIEKKAQANEQRYSKLKEKYSELVQNHADLLRKNA
  EVTKQVSVARQAQVDLEREKKELADSFARTQEQQDVLETLKHELATSRQELQVLHGNLETSAQSEAKWLT
  QIAELEKEQGSLATAAAQREEEFSALRDQLESTQLKLAGAQDSMCQQIKDQRRILLAGARKAAELEIQEA
  LGQLEEPALLISCAGATDHLLSKVNSVSSCLEQLEKSRSQYLACPEEISELLHSITLLGHLTSDTIIQGT
  ATSLRAPPESADSLTEACRQYGRETLAYLSSLEEEGAMEKADTTAIANCLGKIKTIGEELLPRGLDIKQE
  ELGDLVDKEMAATSAAIEAATTRIEEILSKSRAGDTGVKLEVNERILGSCTSLMQAIKVLVVASKDLQKE
  IVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATIMVDAADLVVQGKGKFEELMVCSHEIAASTAQL
  VAASKVKANKGSLNLTQLQQASRGVNQATAAVVASTISGKSQIEETDSMDFSSMTLTQIKRQEMDSQVRV
  LELENDLQKERQKLGELRKKHYELAGVAEGWEEGTEASPSAVQEAVPDKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100475
• ORF Size: 3090 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001100475.1, NP_001093945.1
• RefSeq Size: 7376
• RefSeq ORF: 3093
• Locus ID: 192154
• MW: 115 kDa
• Gene Summary: human homolog binds clathrin and the clathrin adaptor AP2; may play a role in clathrin-mediated endocytosis [RGD, Feb 2006]