Anks3 (NM_001009676) Rat Tagged ORF Clone

CAT#: RR214208

Anks3 (Myc-DDK-tagged ORF) - Rat ankyrin repeat and sterile alpha motif domain containing 3 (Anks3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Anks3
• Synonyms: RGD1305833
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214208 representing NM_001009676
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGCTCAGCGATGAAGCCAGCGAGCCGGAGCTTCTGAACCGCAGCTTGTCCATGTGGCATGGAC
  TTGGTGCACAGGTCAGCAGAGAGGAGCTGGATGTCCCCTTGGATCTTCACACAGCTGCCTCCATTGGCCA
  GTATGAGGTGGTGAAGGAGTGTGTGCAGCGGAGAGAGTTAGATTTGAATAAGAAGAATGGTGGTGGCTGG
  ACTCCGCTGATGTATGCCTCCTACATTGGACACGATACCATTGTTCACTTGCTGCTCGAGGCAGGTGTGA
  GTGTGAATGTGCCAACCCCAGAAGGACAGACTCCACTGATGCTGGCCTCCAGCTGTGGTAACGAGAGCAT
  CGCCTACTTCCTGCTCCAGCAAGGTGCAGAGCTTGAGATGAAAGACATCCAGGGCTGGACTGCACTCTTT
  CACTGCACCAGTGCCGGCCACCAGCAGATGGTCAAGTTTCTTCTGGAGAGTGGAGCCAATGCCAACGTGA
  GGGAACCTGTATATGGATTCACTCCTTTGATGGAAGCAGCTGCCGCTGGCCATGAGATAATTGTGCAGTA
  TTTTCTGAATCATGGAGTCAAAGTGGACACAAGAGACCACAGTGGAGCCACAGCCCGGATGCTAGCCAAG
  CAGTATGGTCACATGAAGATTGTGGCTTTGATGGAAACTCACTCACCTGTACTGCCCAAGAGCCTCTACC
  GTAGCTCAGAAAACTATGAAGACCTGAGCTCTTCAGATGAGTCCTGGCCTGTTCCTCAGCGCCAGAGGCC
  CTGTCGAAAGAAGGGCCTGAGTATCCACGAGGGGCCTCGCGCCCTGGCCAGGATCACAGCTACTGGACTG
  GGAGGCAAGACTCCGGACAGCTATGAGCAAGTTCCTCCACGGGGCTATGTGACCTTCACCAGCAGTGATG
  AGAACACCATGGAAGGCGAGGGCCTCTGCTACCGAGATGTCACTTCCCCCATCAATGAACGCGATGTGGA
  GAGCAGTAGCAGCAGCAGCCGGGAGGAACCCACTTTCTGTGCCAGTCTTGGGCCTGTGTGGAGGAGCAGC
  AGCAGTGATGGCCTAGCAAGAGCCCAGGGTCTCAGCAGTGAAGCCTCCATAGAGAGCAATGAGGATTCTG
  ATCACGCCCGCAAAAGCTCGGTTCGTAAACAAACCAGGACTTATTTGAAGAATAAGAGTCGTCACAACAA
  CAGTGATGGTCACTGGCCTTCTAGCACAGGGACTGCTCGCACTCCAGGGTCTGAACCCCAAGCTGAGAAG
  TCCCCTTACTCAGGACCCCAGGATCTTGCCACACTGTTGGAACAGATCGGCTGTCTAAAGTACCTGCAAG
  TGTTTGAGGAGCAGGATGTAGACCTGCGTATCTTTCTGACCCTCACTGAAAGCGACCTGAAGGAAATTGG
  CATCACGTTGTTTGGGCCCAAAAGGAAAATGACCTCTGCCATTGCCCGATGGCACAGTAGTGCCCGCCCA
  CCTAGTGATGCCCTAGAGCTGGCCTATGCTGACCGGCTGGAGGCGGAGATGCAGGAGCTTGCCATCCAGC
  TGCACAAATGCTGTGAGGAGGCAGAGGCTCTGCGTGGCCAAGTCTCCCAGGAGCAGGAATTGCGGGCTGT
  AGTAGAGAGCTGCCTCCTGGAGCAGGATAGTGCCCGAAAGGACATCCATGCCCAGCTGCAGGAGGCCCAA
  ACCCTGGCCCAGGATGCTGCTCTTGTCCTAGACCAGCTGCGGGCCTGTCAAGCTGAGTTGTCAGCTCGAT
  TGAAGCAGCACCACTCCCCCAGCGAAGCTACCCAAAACCCACCCTTTCTCCCAGCAGATTCCAAAGGCTG
  GCCTATTCCCCTGCAGGCCTTGAGCCTCCCTGAGTTGTCAGGAGCCCTGGAAGACCGTGTCCATGAGATG
  GGCATTATGCTCCGTAACGCAGAGCCTGGAGAAACTACAGATGCTGAATGGGAAGAAATGGAGGGAACCA
  TAGCCAGGAGGGACGATTCTGACGTTGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214208 representing NM_001009676
  Red=Cloning site Green=Tags(s)
  
  MSELSDEASEPELLNRSLSMWHGLGAQVSREELDVPLDLHTAASIGQYEVVKECVQRRELDLNKKNGGGW
  TPLMYASYIGHDTIVHLLLEAGVSVNVPTPEGQTPLMLASSCGNESIAYFLLQQGAELEMKDIQGWTALF
  HCTSAGHQQMVKFLLESGANANVREPVYGFTPLMEAAAAGHEIIVQYFLNHGVKVDTRDHSGATARMLAK
  QYGHMKIVALMETHSPVLPKSLYRSSENYEDLSSSDESWPVPQRQRPCRKKGLSIHEGPRALARITATGL
  GGKTPDSYEQVPPRGYVTFTSSDENTMEGEGLCYRDVTSPINERDVESSSSSSREEPTFCASLGPVWRSS
  SSDGLARAQGLSSEASIESNEDSDHARKSSVRKQTRTYLKNKSRHNNSDGHWPSSTGTARTPGSEPQAEK
  SPYSGPQDLATLLEQIGCLKYLQVFEEQDVDLRIFLTLTESDLKEIGITLFGPKRKMTSAIARWHSSARP
  PSDALELAYADRLEAEMQELAIQLHKCCEEAEALRGQVSQEQELRAVVESCLLEQDSARKDIHAQLQEAQ
  TLAQDAALVLDQLRACQAELSARLKQHHSPSEATQNPPFLPADSKGWPIPLQALSLPELSGALEDRVHEM
  GIMLRNAEPGETTDAEWEEMEGTIARRDDSDVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009676
• ORF Size: 1989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009676.1, NP_001009676.1
• RefSeq Size: 2419 bp
• RefSeq ORF: 1992 bp
• Locus ID: 302937
• Cytogenetics: 10q12
• MW: 73 kDa