Col4a4 (NM_001008332) Rat Tagged ORF Clone

CAT#: RR214290

Col4a4 (Myc-DDK-tagged ORF) - Rat procollagen, type IV, alpha 4 (Col4a4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col4a4
• Synonyms: MGC105542
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214290 representing NM_001008332
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGCTTTTTCAGATGGACCGAGTCCTACGGCACAGGCCCCTGGTCACTGATATTTATCCTCTTTA
  CCATACAATATGAATATGGGAGCAGCAAGAAATATGGCAGCCCCTGTGGAGGAAGAAACTGCTCTGTCTG
  CCAGTGTTTCCCTGAAAAGGGATCTCGGGGTCACCCAGGACCACTGGGACCACAGGGACCAATAGGGCCC
  TTGGGACCCCCAGGACCCATTGGGATTCCAGGAGAAAAAGGACTGAGAGGTGACAGCGGCCTGCCTGGAC
  CACCAGGAGAGAAAGGAGACAAGGGTCCAACTGGTGTGCCCGGATTTCCAGGCGTGGATGGTGTACCTGG
  TCACCCAGGGCCTCCTGGACCCAGAGGCAAACCTGGTATGGACGGCTACAATGGCTCACGAGGTGATCCG
  GGCTATCCAGGAGAGAGAGGAGCTCCTGGCCCTGGCGGCCCCCCTGTAAGACACCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214290 representing NM_001008332
  Red=Cloning site Green=Tags(s)
  
  MRCFFRWTESYGTGPWSLIFILFTIQYEYGSSKKYGSPCGGRNCSVCQCFPEKGSRGHPGPLGPQGPIGP
  LGPPGPIGIPGEKGLRGDSGLPGPPGEKGDKGPTGVPGFPGVDGVPGHPGPPGPRGKPGMDGYNGSRGDP
  GYPGERGAPGPGGPPVRHH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008332
• ORF Size: 477 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008332.1, NP_001008333.1
• RefSeq Size: 1190 bp
• RefSeq ORF: 480 bp
• Locus ID: 301562
• Cytogenetics: 9q34
• MW: 16.2 kDa