Ppp2r3a (NM_001012202) Rat Tagged ORF Clone

CAT#: RR214355

Ppp2r3a (Myc-DDK-tagged ORF) - Rat protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha (Ppp2r3a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp2r3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214355 representing NM_001012202
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAACTTACAGACTCGTGGTTAGCACTGTGAACCATTATAGCAGTGTAGTGATAGACCGGCGTT
  TTGAACAAGCCATACATTATTGCACTGGAACCTGCCATACCTTCACACATGGAATAGACTGCATTGTAGT
  GCATCATAGTGTTTGTGCAGACCTTTTGCACATCCCTGTGTCTCAGTTCAAAGATGTGGATCTGAACTCT
  GTGTTTTTGTCTCATGAAAATGGCCTTTCCTCAGCTGAAGACTATCCCCATCAGGCCCTCACAGGAATAC
  CCAGGTCCAAGAGAGCATCCACATTTCAGAATACCTGTAACTTAAAGGACATTGCAGGAGAAGCAATCAG
  CTTTGCCAGTGGGAAAATAAAAGAATTTTCCCTTGAAAAACTCAGAAATTCAAATCATGCAGCTTACAAA
  AAAGGAAGAAAAGTTAAATCTGACTCATTTAATAGGCGATCAGTTGATTTGGATTTACTCTGTGGCCATT
  ATAGTAACGATGGGACCTCCCCATCCTTTGGCTTACTCCGGAGTTCCTCAGTTGAGGAAAAATCTTTATC
  TCATAGAAACTCACTAGATACAAGCCTAACTTCCATGCTTTTTCAGAACTTGTCTGAAGAAGATCTAGTT
  ACGCAGATTTTGGAAAAACATAAAATAGATCATTTTTCTTCTGGGACAGACATAAAAATGTGCTTGGACA
  TCTTACTGAAATGCTCTGAAGATTTGAAAAAATGTACAGACATCATAAAACAATGCATAAAGAAAAAGTC
  AGGAGGAAGCATCAACGAAGGAAGTGGCAGTGACGCAATGTCTAACTCTGAAACCGTCTATATGAATGTA
  ATGGCCAGGTTAGCTTACTACCTGAGAAAGTTACCATTTGAATTTGTGCAGTCTGGGAGTAATGAGACTG
  TAGATTTGACAGAGCTTGTCAATAATGTACCTAACTTACAACTGACTCCATTCTCCCCATTATATGGGAC
  TGAACAGCCCCCTAAATATGAAGATATCGTCCACCTCTCAGCCCTTGACTCCAGACAGTTTCAGACTGTT
  GAATTGCAAGATGACATGCATAATTTTAAACAAACAGATACTGTAAAAACCCCTCCAAACAACACAAATT
  CCTTGTGTAGCTTGGAGGTAAACGATCCTGGAACTCTAAAACCTGTGCAGCTTCAATCACCGTCATTAAC
  CATAAACCATTTAGAGAATGTTTCTTCTGGTAACTTAATGGAAACTCTTTATATTGAAGAAGAGTCAGAT
  GGAAAGAAGTCATTATTAGATAAAGGACAAAAAGCAGATAATGGACAAGGTCAGGAACCATTAAAGGTAA
  ATGAACATAAAGAATTTTCAGACCATGATACTTATCTTAAAAAATGTCCTGCCCCAGTGCAAAATGAAAT
  CAGTAAGATATTTGAAAAGCCAATGGTAGTTCGTCCACCTAAGGAAGAATCTGAATTAGCAGTTCCTCCT
  ACAGAACTCAGAGCCCAGAGGCACTTTATGAATCCTAACAGTCAAGAAGAGATCGACAAATTGTTAATGG
  ATTTGGAATCATTCTCACAGAAGATGGAGACCTCTCTAGGAGAACCACTTGCCAAGGGTAAAAGCATCAA
  TTCTCTAAATAGTCATAGTCATTTGACTGGTCAGATCCACAAAGATCTTGAGCCTAAACCTATAGTCTCT
  TCAGCTACGGAGAAAGTCTCACCTTCTTGTCTAACAAGGATTATTGAAAACAACGGACACAAAATAGAGG
  AAGAGGACCGAGCCCTCTTACTTCGAATTCTGGAAAGCATTGAAGACTTTGCTCAAGAACTAGTTGAATG
  CAAGTCAAGCAGAGGGAGCCTATCACAAGAAAAGGAAATGATGCAAATTCTGCAGGAAACTTTGACAGCT
  TCCTCCCAGCCCACTCCATCAGTCAGTAGGAGCCCTGTTGGTGAGAAACCCAAAGATAGTACTTCAGTTG
  TTTTGATTCAACAGACACCAGAAGTGATCAAGATTCAAAATAAACCAGAAAAAAAACCTGCAACACCATG
  TCCACCTGCAGCCACCCTTCCAAGTAGTCCCCAGCCTGCCTCCCCTGTTCCTTGTGTGAGCGATGTCGTG
  AATACACCACTGTCCATAAACATTCCACAGTTCTACTTTCCCGAAGGACTCCCAGACACCTGCAGCAACC
  ACGAACAGATCCTAGGCAGAATTGAAGCTGCTTTCAAGGATATTGAAGATCAAAAAGCAGATATTCATGA
  AATGGGGAGAATTGCAAAGGCCTGCGGCTGTCCACTCTATTGGAAAGCCCCCATGTTCAGGGCTGCAGGA
  GGAGAGAAGACTGGATTTGTGACAGCACAGTCATTCATGGCCATGTGGAAAAAGTTGCTAAGTGACCATC
  ATGATGATGCCTCCAGGTTCATCTGGCTTCTGGCAAAGCCCAGCTGCAGCCATCTCCAACAAGAGGATTT
  TATCCCTCTACTTCAGGATGTGGTAGATACACACCCTGGCCTCACCTTCCTGAAAGATGCTCCTGAATTC
  CATTCCCGCTACATCACCACGGTTGTTCAGAGAATATTCTACACAGTCAACAGATCTTGGAGTGGAAAAA
  TTACTTCAACAGAGATAAGGAAAAGCAACTTTTTGCAAACTCTGGCCCTTTTGGAGGAAGAGGAAGATAT
  AAACCAAATCACAGATTACTTCTCTTATGAACATTTCTATGTCATTTATTGTAAATTCTGGGAACTAGAT
  GGTGATCATGACCTATACATCAGCCAAGCTGATCTCTCTCGATACAATGACCAGGCTTCATCAAATAGAA
  TTATTGAAAGAATATTTTCTGGAGCAGTAACAAGAGGGAAAACTGTACAAAGAGAGGGAAGAATGAGCTA
  TGCAGATTTTGTTTGGTTTTTGATTTCTGAGGAAGACAAACGGAACCCTACCAGCATTGAGTATTGGTTC
  CGCTGCATGGATGTGGATGGAGATGGTGTACTTTCCATGTATGAACTAGAGTATTTCTATGAAGAGCAGT
  GTGAACGGATGGAAGCCATGGGCATCGAGCCCTTGCCGTTTCATGATCTGTTGTGCCAGATGCTAGACTT
  GGTGAAGCCAGCTAATGATGGCAGAATAACTCTGCGAGATTTGAAGAGGTGCAGAATGGCTCACATCTTC
  TACGACACTTTCTTTAACCTGGAGAAGTACTTGGACCATGAACAAAGAGATCCTTTTGCAGTCCAGAAGG
  ATGTTGAAAATGAGGGCCCCGAGCCCTCTGACTGGGACAGGTTTGCTGCTGAGGAGTACGAGGCTCTGGT
  GACTGAGGAATCTGCCCAGGTTCAGCTCCAGGAAGGCTCATTTGAAGACTATGAATCAGATGAGCCTGTG
  TCTCCCTCTGAACTTGGAAACAAGGGCAATAAAATCCTAACTTCAAGCCTTTCAGAGAAATGTGGAAAGC
  TCCAGTCAGTGGATGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214355 representing NM_001012202
  Red=Cloning site Green=Tags(s)
  
  MAATYRLVVSTVNHYSSVVIDRRFEQAIHYCTGTCHTFTHGIDCIVVHHSVCADLLHIPVSQFKDVDLNS
  VFLSHENGLSSAEDYPHQALTGIPRSKRASTFQNTCNLKDIAGEAISFASGKIKEFSLEKLRNSNHAAYK
  KGRKVKSDSFNRRSVDLDLLCGHYSNDGTSPSFGLLRSSSVEEKSLSHRNSLDTSLTSMLFQNLSEEDLV
  TQILEKHKIDHFSSGTDIKMCLDILLKCSEDLKKCTDIIKQCIKKKSGGSINEGSGSDAMSNSETVYMNV
  MARLAYYLRKLPFEFVQSGSNETVDLTELVNNVPNLQLTPFSPLYGTEQPPKYEDIVHLSALDSRQFQTV
  ELQDDMHNFKQTDTVKTPPNNTNSLCSLEVNDPGTLKPVQLQSPSLTINHLENVSSGNLMETLYIEEESD
  GKKSLLDKGQKADNGQGQEPLKVNEHKEFSDHDTYLKKCPAPVQNEISKIFEKPMVVRPPKEESELAVPP
  TELRAQRHFMNPNSQEEIDKLLMDLESFSQKMETSLGEPLAKGKSINSLNSHSHLTGQIHKDLEPKPIVS
  SATEKVSPSCLTRIIENNGHKIEEEDRALLLRILESIEDFAQELVECKSSRGSLSQEKEMMQILQETLTA
  SSQPTPSVSRSPVGEKPKDSTSVVLIQQTPEVIKIQNKPEKKPATPCPPAATLPSSPQPASPVPCVSDVV
  NTPLSINIPQFYFPEGLPDTCSNHEQILGRIEAAFKDIEDQKADIHEMGRIAKACGCPLYWKAPMFRAAG
  GEKTGFVTAQSFMAMWKKLLSDHHDDASRFIWLLAKPSCSHLQQEDFIPLLQDVVDTHPGLTFLKDAPEF
  HSRYITTVVQRIFYTVNRSWSGKITSTEIRKSNFLQTLALLEEEEDINQITDYFSYEHFYVIYCKFWELD
  GDHDLYISQADLSRYNDQASSNRIIERIFSGAVTRGKTVQREGRMSYADFVWFLISEEDKRNPTSIEYWF
  RCMDVDGDGVLSMYELEYFYEEQCERMEAMGIEPLPFHDLLCQMLDLVKPANDGRITLRDLKRCRMAHIF
  YDTFFNLEKYLDHEQRDPFAVQKDVENEGPEPSDWDRFAAEEYEALVTEESAQVQLQEGSFEDYESDEPV
  SPSELGNKGNKILTSSLSEKCGKLQSVDEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012202
• ORF Size: 3450 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012202.2, NP_001012202.2
• RefSeq Size: 5212 bp
• RefSeq ORF: 3453 bp
• Locus ID: 363122
• Cytogenetics: 8q31
• MW: 129.9 kDa