Loxl1 (NM_001012125) Rat Tagged ORF Clone

CAT#: RR214661

Loxl1 (Myc-DDK-tagged ORF) - Rat lysyl oxidase-like 1 (Loxl1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Loxl1
• Synonyms: MGC105563
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214661 representing NM_001012125
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGCCGGAGCCGGCAGCCAGCTGGGGACCCTGGTGTGGGGAGCCTGCCTGTGCGTCCTGGTGC
  ACGGGCAGCAAGAGCAGCCAGGACAGGGCTCGGACCCAGGACGCTGGCGGCAGCTGATCCAGTGGGAGAA
  CAACGGGCAGGTGTACAGCTTGCTCAATTCGGGCTCTGAGTACGTGCCTGCGGGGCCCCAAAGAGGGGAG
  AGTAGCTCCAGGGTGCTACTGGCAGGCGCGCCCCAAACGTCGCAGCGGCGCAGTCAGGGAGGACCCCGGC
  GTCGGCAGGCGCCGAACCTGCCCCTGCCCGGACGCGTGGGTTCGGACACTGTGCGCGGCCAAACGCGGCA
  CCCGTTCGGCTTCGGCCAGGTGCCAGACAACTGGAGAGAGGTGGCCGTCGGGGACAGTAACGGGATGGCT
  CGGGCCCGCACCTCCGTCTCCCAGCAACGGCACGGGGGCTCCGCCTCCTCCTCGGTTTCGGCCTCGGCCT
  TCGCCACCACTTATCGCCAGCCATCCTCCTACCCGCAGCAGTTCCCCTATCCGCAGGCGCCCTTCGTGAA
  CCAATATGAGAACTATGACCCCGCGTCGCGGACCTATGAACAGGGCTACGTGTACTACCGCGGTGCGGGC
  GGAGGCATGGGCGCAGGGGCGGCGGCGGCAGCTGTAGCCTCGGCTGGGGTCATCTATCCCTTCCAGCCCC
  GGGCACGCTACGAGGAGTACGGCGGTGGGGGCGAGGAACAGCCCGAGTACCCGGCGCAGGGCTTCTACCC
  AGCCCCAGAGAGGCCCTATGCGCCCCAGCCCCAGCCTCAGCCCCAACCTCAGCCGCAGCCTCAGCCCCAA
  CCTTCCGACGGCCTTGACCGTCGTTACTCGCATAGCCTGTACAATGAAGGCACCCCGGGATTCGAGCAGG
  CCTACCCTGATCCCAGCACCGACGTGTCGCAGATCCCCGCCGGGGCCGGTGGCACCTACGGTGCCGCTGG
  CGATCCCCGTCTCGGCTGGTACCCGCCCTATGCAGCCAATGTGCCTCCCGAGGCCTATGTCCCTCCGCGT
  GCGGTGGAGCCGCAGCCCCCATTCCGCGTGCTGGAACCACCTTACTTGCCTGTGCGAAACTCTGATGCGC
  CCTCCCAGGGTGGGGAGAGGAACGGTGCCCAGCAGGGCCGTCTCAGCGTGGGTAGTGTGTACCGACCTAA
  CCAGAATGGTCGCGGCCTCCCTGACTTAGTCCCAGATCCCAACTACGTGCAGGCATCCACATATGTGCAG
  AGAGCCCATCTGTATTCCTTGCGTTGTGCGGCAGAGGAGAAGTGCCTGGCCAGCACAGCCTATGCTCCTG
  AGGCCACCGACTATGACCTCCGAGTGCTGTTACGATTCCCCCAGCGTGTGAAAAACCAGGGCACGGCGGA
  CTTCCTCCCCAACCGGCCACGCCACACCTGGGAGTGGCATAGCTGTCACCAACATTACCACAGCATGGAC
  GAGTTCAGCCACTATGACCTACTTGATGCATCCACGGGCAAGAAAGTTGCCGAGGGACACAAGGCCAGCT
  TTTGCCTGGAGGACAGCACCTGTGACTTCGGCAACCTCAAGCGCTATGCGTGCACCTCACACACGCAGGG
  CCTGAGTCCAGGCTGCTATGACACCTACAATGCGGACATCGACTGCCAGTGGATCGACATAACAGATGTG
  CAGCCTGGGAACTACATCCTCAAGGTGCACGTGAACCCCAAGTACATTGTTCTGGAGTCTGACTTCACCA
  ACAACGTGGTGAGATGCAACATCCACTACACAGGTCGCTTCGTCTCTACAACAAACTGCAAAATCGTCCA
  GTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214661 representing NM_001012125
  Red=Cloning site Green=Tags(s)
  
  MALAGAGSQLGTLVWGACLCVLVHGQQEQPGQGSDPGRWRQLIQWENNGQVYSLLNSGSEYVPAGPQRGE
  SSSRVLLAGAPQTSQRRSQGGPRRRQAPNLPLPGRVGSDTVRGQTRHPFGFGQVPDNWREVAVGDSNGMA
  RARTSVSQQRHGGSASSSVSASAFATTYRQPSSYPQQFPYPQAPFVNQYENYDPASRTYEQGYVYYRGAG
  GGMGAGAAAAAVASAGVIYPFQPRARYEEYGGGGEEQPEYPAQGFYPAPERPYAPQPQPQPQPQPQPQPQ
  PSDGLDRRYSHSLYNEGTPGFEQAYPDPSTDVSQIPAGAGGTYGAAGDPRLGWYPPYAANVPPEAYVPPR
  AVEPQPPFRVLEPPYLPVRNSDAPSQGGERNGAQQGRLSVGSVYRPNQNGRGLPDLVPDPNYVQASTYVQ
  RAHLYSLRCAAEEKCLASTAYAPEATDYDLRVLLRFPQRVKNQGTADFLPNRPRHTWEWHSCHQHYHSMD
  EFSHYDLLDASTGKKVAEGHKASFCLEDSTCDFGNLKRYACTSHTQGLSPGCYDTYNADIDCQWIDITDV
  QPGNYILKVHVNPKYIVLESDFTNNVVRCNIHYTGRFVSTTNCKIVQS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012125
• ORF Size: 1824 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012125.1, NP_001012125.1
• RefSeq Size: 2491 bp
• RefSeq ORF: 1827 bp
• Locus ID: 315714
• Cytogenetics: 8q24
• MW: 66.6 kDa