Hk1 (NM_012734) Rat Tagged ORF Clone

CAT#: RR214718

Hk1 (Myc-DDK-tagged ORF) - Rat hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hk1
• Synonyms: HEXOKIN; HKI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214718 representing NM_012734
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCCGCGCAACTACTGGCCTATTACTTCACCGAGCTGAAGGATGACCAAGTCAAAAAGATTGACA
  AGTATCTGTACGCCATGCGGCTCTCTGATGAGATTCTGATAGATATCCTGACACGATTCAAGAAAGAGAT
  GAAGAATGGCCTCTCCCGGGATTATAATCCAACAGCCTCCGTCAAGATGCTGCCCACCTTCGTCCGGTCC
  ATTCCGGACGGCTCAGAAAAGGGGGATTTCATTGCCCTGGATCTCGGCGGGTCTTCCTTTCGAATCCTGC
  GGGTGCAGGTGAACCACGAGAAGAACCAGAACGTCAGCATGGAGTCTGAGATCTACGACACCCCAGAGAA
  CATCGTGCATGGCAGTGGAACCCAGCTTTTCGATCATGTCGCTGACTGCCTGGGAGACTTCATGGAGAAA
  AAGAAGATCAAGGACAAGAAGTTACCCGTGGGATTCACATTTTCCTTCCCCTGCCGACAATCCAAGATAG
  ATGAGGCTGTACTGATCACGTGGACAAAGCGGTTCAAAGCCAGTGGCGTGGAAGGAGCGGATGTGGTCAA
  GTTGCTGAATAAAGCCATTAAGAAGCGAGGGGACTATGATGCTAACATTGTCGCCGTGGTGAATGACACA
  GTAGGGACCATGATGACCTGCGGTTATGATGACCAACAGTGTGAAGTCGGCCTGATCATTGGCACAGGCA
  CCAATGCTTGCTACATGGAGGAACTGCGACACATCGACCTGGTGGAAGGCGACGAGGGGAGGATGTGTAT
  TAACACGGAATGGGGAGCCTTTGGGGATGATGGGTCCCTGGAAGACATCCGAACCGAGTTTGACAGAGAG
  TTAGACCGTGGATCTCTCAACCCTGGGAAGCAGCTGTTCGAGAAGATGGTGAGCGGCATGTACATGGGGG
  AGCTGGTCCGGCTAATCCTGGTGAAGATGGCCAAGGAAGGCCTCTTATTCGAAGGGCGCATCACTCCAGA
  GCTGCTCACGAGGGGAAAGTTCAACACTAGTGACGTGTCCGCCATTGAAAAGGATAAGGAAGGCATTCAA
  AATGCCAAGGAAATCTTAACCCGCTTGGGAGTGGAGCCGTCTGATGTTGACTGTGTGTCGGTCCAGCACA
  TCTGCACGATCGTCTCCTTCCGATCAGCCAACCTGGTGGCCGCCACGCTCGGTGCCATCTTGAACCGCCT
  GCGGGACAACAAGGGCACACCACGCCTGCGGACCACGGTTGGCGTGGACGGTTCTCTCTACAAGATGCAC
  CCACAGTACTCCCGGCGGTTCCACAAGACCCTGAGGCGCCTGGTGCCTGACTCCGACGTCCGTTTCCTCC
  TCTCAGAGAGTGGCACGGGCAAGGGGGCCGCCATGGTGACGGCAGTAGCCTACCGCCTGGCTGAGCAGCA
  CCGGCAGATTGAGGAAACCCTGGCCCACTTCCGCCTCAGCAAGCAGACGCTGATGGAGGTGAAGAAGAGG
  CTACGGACAGAGATGGAAATGGGGCTGAGGAAGGAGACCAACAGCAAAGCTACTGTCAAAATGCTGCCTT
  CTTTTGTCCGGAGCATCCCGGATGGGACTGAACACGGTGACTTCCTGGCCTTGGATCTTGGAGGAACGAA
  TTTCCGGGTTCTGCTGGTAAAGATCCGCAGTGGGAAAAAGAGAACAGTGGAAATGCACAACAAGATCTAC
  TCCATTCCCCTGGAAATCATGCAGGGCACCGGGGATGAGCTGTTTGACCACATCGTCTCCTGCATCTCTG
  ACTTCCTGGACTACATGGGGATCAAAGGCCCCCGGATGCCTCTGGGCTTCACCTTCTCATTTCCCTGCCA
  TCAGACGAACCTGGACTGTGGAATCTTGATCTCATGGACAAAGGGTTTCAAAGCCACTGACTGTGAGGGC
  CATGATGTAGCCTCCTTACTGAGGGATGCGGTGAAGAGGAGAGAGGAATTTGACTTGGATGTGGTGGCTG
  TGGTCAACGACACCGTGGGCACCATGATGACCTGTGCGTATGAAGAACCCACTTGCGAAATTGGACTCAT
  CGTGGGGACGGGCACCAATGCCTGCTACATGGAGGAGATGAAGAATGTGGAGATGGTGGAGGGGAACCAG
  GGCCAGATGTGCATCAACATGGAGTGGGGCGCCTTCGGTGACAATGGGTGTCTGGATGACATCAGAACAG
  ACTTTGACAAAGTGGTGGACGAATATTCTCTAAACTCTGGGAAACAAAGGTTTGAGAAAATGATCAGTGG
  GATGTACCTGGGTGAGATCGTCCGTAACATCCTGATTGACTTCACCAAGAAAGGCTTCCTCTTCCGGGGA
  CAGATCTCCGAACCACTCAAGACCCGAGGCATCTTTGAGACCAAGTTTCTCTCTCAGATTGAGAGTGACC
  GGTTAGCGCTGCTCCAGGTGCGGGCCATCCTTCAGCAGCTGGGTTTGAACAGCACGTGTGACGACAGTAT
  CCTGGTCAAGACCGTGTGTGGGGTGGTGTCCAAGAGGGCGGCTCAGCTGTGTGGTGCCGGCATGGCCGCC
  GTGGTGGAAAAGATCAGAGAGAACAGAGGCCTAGACCATCTGAATGTAACTGTGGGAGTGGATGGGACGC
  TCTACAAACTTCATCCACACTTCTCCAGAATCATGCACCAAACTGTGAAGGAACTGTCACCAAAGTGTAC
  CGTGTCCTTCCTCCTGTCTGAAGACGGCAGCGGCAAGGGGGCCGCCCTTATCACAGCTGTGGGCGTGCGG
  CTCAGAGGAGACCCTTCGATCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214718 representing NM_012734
  Red=Cloning site Green=Tags(s)
  
  MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDEILIDILTRFKKEMKNGLSRDYNPTASVKMLPTFVRS
  IPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVSMESEIYDTPENIVHGSGTQLFDHVADCLGDFMEK
  KKIKDKKLPVGFTFSFPCRQSKIDEAVLITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDT
  VGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDRE
  LDRGSLNPGKQLFEKMVSGMYMGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKDKEGIQ
  NAKEILTRLGVEPSDVDCVSVQHICTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKMH
  PQYSRRFHKTLRRLVPDSDVRFLLSESGTGKGAAMVTAVAYRLAEQHRQIEETLAHFRLSKQTLMEVKKR
  LRTEMEMGLRKETNSKATVKMLPSFVRSIPDGTEHGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIY
  SIPLEIMQGTGDELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCHQTNLDCGILISWTKGFKATDCEG
  HDVASLLRDAVKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEIGLIVGTGTNACYMEEMKNVEMVEGNQ
  GQMCINMEWGAFGDNGCLDDIRTDFDKVVDEYSLNSGKQRFEKMISGMYLGEIVRNILIDFTKKGFLFRG
  QISEPLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAA
  VVEKIRENRGLDHLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCTVSFLLSEDGSGKGAALITAVGVR
  LRGDPSIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012734
• ORF Size: 2754 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012734.1, NP_036866.1
• RefSeq Size: 3653
• RefSeq ORF: 2757
• Locus ID: 25058
• MW: 102.4 kDa
• Gene Summary: a ATP:D-hexose 6-phosphotransferase involved in the phosphorylation of glucose to produce glucose-6-phosphate [RGD, Feb 2006]