Srd5a1 (NM_017070) Rat Tagged ORF Clone

CAT#: RR214744

Srd5a1 (Myc-DDK-tagged ORF) - Rat steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) (Srd5a1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srd5a1
• Synonyms: S5AR 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214744 representing NM_017070
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGGATGAGCTGTGCCTGCTCGACATGCTGGTCTACTTGGAAGGTTTCATGGCCTTCGTGTCCA
  TTGTGGGGCTCCGGTCGGTTGGCTCTCCGTACGGCCGCTACTCGCCGCAGTGGCCCGGCATCCGAGTGCC
  CGCGCGACCTGCCTGGTTCATACAGGAGCTGCCCTCGATGGCCTGGCCGCTGTACGAGTACATTCGTCCT
  GCAGCCGCGCGACTGGGCAACCTGCCTAACCGCGTCCTGCTGGCTATGTTTCTGATCCACTACGTGCAAA
  GGACGCTGGTTTTCCCGGTTCTGATCAGGGGAGGGAAGCCCACCCTCCTGGTCACCTTTGTCTTGGCCTT
  CCTGTTCTGCACCTTCAACGGCTATGTACAGAGCAGATACTTGAGCCAGTTTGCGGTTTATGCTGAAGAC
  TGGGTGACCCATCCCTGTTTCCTGACAGGCTTTGCCCTGTGGTTAGTGGGCATGGTGATAAATATCCACT
  CAGACCACATCCTGAGGAATCTGAGAAAACCAGGGGAAACTGGATACAAGATACCCAGGGGAGGCCTGTT
  TGAATACGTATCTGCAGCCAACTATTTTGGGGAGCTCGTGGAGTGGTGTGGCTTTGCACTGGCCAGCTGG
  TCCCTCCAGGGTGTAGTGTTTGCACTGTTCACACTCAGCACACTGCTCACCAGAGCGAAGCAGCACCATC
  AGTGGTACCATGAGAAGTTTGAAGATTACCCCAAGTCAAGAAAAATACTGATTCCATTTGTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214744 representing NM_017070
  Red=Cloning site Green=Tags(s)
  
  MELDELCLLDMLVYLEGFMAFVSIVGLRSVGSPYGRYSPQWPGIRVPARPAWFIQELPSMAWPLYEYIRP
  AAARLGNLPNRVLLAMFLIHYVQRTLVFPVLIRGGKPTLLVTFVLAFLFCTFNGYVQSRYLSQFAVYAED
  WVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALASW
  SLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017070
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017070.2, NM_017070.3, NP_058766.2
• RefSeq Size: 2638
• RefSeq ORF: 768
• Locus ID: 24950
• MW: 29.3 kDa
• Gene Summary: catalyzes the conversion of testosterone to dihydrotestosterone; required for male sex differentiation [RGD, Feb 2006]