Plcb3 (NM_033350) Rat Tagged ORF Clone

CAT#: RR214977

Plcb3 (Myc-DDK-tagged ORF) - Rat phospholipase C, beta 3 (phosphatidylinositol-specific) (Plcb3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plcb3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214977 representing NM_033350
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCGAGGCCCGGCGTCCACGCGCTGCAGCTGGAGCCGCCCACCGTGGTGGAGACCCTGCGGC
  GCGGGAGTAAATTCATCAAATGGGACGAGGAGGCCTCCAGTCGGAACCTGGTGACCCTGCGAGTGGACCC
  CAATGGCTTCTTCTTGTACTGGACAGGACCCAACATGGAGGTGGACACACTGGACATCAGCTCCATCAGG
  GACACAAGGACAGGTCGTTATGCCCGCCTGCCCAAGGACCCCAAGATTCGAGAGGTACTGGGCTTTGGGG
  GTCCTGACACCCGGCTGGAGGAGAAACTGATGACAGTGGTAGCAGGACCAGATCCAGTAAACACCACATT
  CTTGAACTTCATGGCCGTGCAAGATGACACAGTCAAGGTCTGGTCAGAGGAGTTGTTTAAGCTGGCTATG
  AACATACTGGCTCAGAACGCCTCCCGGAACACGTTCCTTCGGAAAGCATACACGAAACTGAAGCTGCAGG
  TGAACCAGGATGGACGGATTCCAGTCAAGAACATTCTGAAGATGTTCTCCGCGGACAAGAAGCGGGTGGA
  GACTGCACTGGAGTCCTGCGGCCTCAACTTCAACCGGAGTGAGTCCATTCGGCCTGATGAGTTTTCCTTG
  GAAATCTTCGAGCGGTTCTTGAATAAGCTGTGTCTGCGACCGGATATTGACAAGATCCTGCTGGAGATAG
  GTGCTAAGGGCAAGCCGTACCTCACTCTGGAGCAGCTCATGGACTTTATCAACCAGAAGCAAAGAGACCC
  AAGACTCAACGAGGTGCTGTACCCACCGCTTCGCTCCTCACAGGCTCGGCTGCTCATTGAGAAGTATGAG
  CCGAACAAACAGTTCCTGGAGCGCGACCAGATGTCTATGGAGGGTTTCAGCCGCTATCTGGGAGGGGAGG
  AGAATGGCATCCTGCCCCTGGAGGCCCTGGATCTGAGCATGGACATGACGCAGCCACTGAGCGCATACTT
  CATCAATTCTTCACACAATACCTATCTCACTGCGGGCCAGCTGGCTGGAACATCGTCAGTGGAGATGTAC
  CGCCAGGCGCTGCTGTGGGGCTGCCGTTGTGTGGAGCTGGATGTATGGAAGGGACGGCCACCGGAGGAAG
  AGCCCTTCATTACCCATGGTTTCACCATGACCACTGAGGTGCCATTGCGTGATGTGTTAGAGGCCATCGC
  CGAGACAGCCTTCAAGACCTCACCCTACCCTGTCATTCTCTCCTTCGAAAACCATGTGGACTCGGCAAAA
  CAGCAGGCCAAGATGGCAGAGTACTGCCGCTCTATCTTTGGGGAAGCGCTGCTCATTGACCCACTGGACA
  AGTACCCGCTGTCTGCGGGCACCCCCCTTCCTAGTCCACAGGACCTGATGGGCCGTATCCTGGTGAAGAA
  TAAGAAGCGGCATCGGCCCAGCACAGGTGTCCCTGACAGCTCCGTGCGCAAGCGGCCTCTGGAACAGAGC
  AATTCAGCCCTGAGTGAGAGCTCAGCTGCCACAGAGCCCTCCTCACCCCAGCTGGGGTCCCCCAGCTCTG
  ACAGCTGCCCTGGCCTAAGCAATGGGGAAGAGGTGGGACTTGAGAAGACCAGCCTGGAGCCTCAGAAGTC
  TCTAGGTGAGGAGGGCCTCAACCGAGGACCTAATGTTCTCATGCCTGACCGTGACCGAGAGGATGAGGAG
  GAAGATGAAGAAGAGGAGGAAACAACGGATCCAAAAAAGCCCACCACCGATGAGGGCACAGCCAGCAGTG
  AGGTCAACGCCACAGAGGAGATGTCGACACTCGTCAATTACGTTGAGCCCGTCAAGTTCAAGTCCTTTGA
  GGCTGCTCGAAAAAGGAACAAGTGCTTTGAGATGTCGTCCTTTGTGGAGACCAAGGCGATGGAGCAGCTG
  ACCAAGAGCCCCATGGAGTTCGTGGAATACAACAAACAGCAGCTTAGTCGCATCTACCCCAAGGGCACCC
  GTGTGGACTCCTCCAACTACATGCCTCAGCTCTTCTGGAACGTGGGCTGTCAGCTTGTTGCCCTCAACTT
  CCAGACCTTGGATTTGCCAATGCAGCTCAACGCAGGCGTGTTTGAGTACAACGGGCGCAGTGGGTACTTG
  CTCAAGCCTGAGTTTATGCGGCGGCCGGACAAGTCTTTTGACCCCTTCACTGAGGTCATCGTAGATGGCA
  TAGTGGCCAACGCCTTGCGGGTCAAGGTGATTTCGGGGCAGTTTCTGTCTGACAGGAAGGTGGGCATCTA
  TGTCGAGGTGGACATGTTTGGTCTCCCAGTTGACACAAGACGCAAATATCGCACACGGACATCCCAGGGG
  AACTCATTCAACCCTGTGTGGGATGAAGAACCCTTTGACTTCCCCAAGGTTGTGCTGCCCACGCTGGCCT
  CACTTCGCATTGCAGCCTTTGAGGAGGGTGGCAGATTTGTTGGGCATCGTATCCTGCCTGTCTCTGCTAT
  CCGCTCAGGATACCACTACGTCTGCCTGCGAAATGAGGCCAACCAACCGTTGTGCCTGCCTGCCCTGCTT
  ATCTACACTGAAGCTTCTGACTACATCCCAGATGACCACCAGGACTATGCGGAGGCCTTGATCAACCCCA
  TCAAGCACGTCAGCCTAATGGACCAGCGGGCCAAGCAACTGGCTGCTCTCATTGGGGAGAGTGAGGCTCA
  GGCCAGTACAGAAATGTGTCAGGAGACCCCGTCTCAACAGCAAGGCTCACAGCTCTCCTCCAACCCCGCA
  CCTAACCCACTGGATGCCTCACCTCGATGGCCCCCAGGTCCTACCACCTCCCCCACTAGCACCTCCCTCA
  GTAGCCCAGGGCAGCGAGATGATCTGATCGCCAGCATCCTTTCTGAGGTGACCCCAACACCCCTAGAGGA
  GCTCCGAAGCCACAAGGCTATGGTGAAGCTCCGGAGCCGGCAGGATCGAGACCTGAGGGAACTGCATAAA
  AAACACCAGCGCAAGGCTGTGGCCCTCACCCGCCGCCTTCTGGATGGCCTGGCCCAGGCCAGGGCTGAGG
  GGAAGTGCCGGCCCTCCTCCAGCGCCCTGAGCAGGGCCACCAATGTGGAAGACGTGAAGGAGGAAGAAAA
  AGAGGCAGCCAGACAGTATCGAGAGTTCCAGAACCGACAAGTACAGAGCCTGCTGGAGCTGAGGGAGGCC
  CAGGCAGATGCAGAGACAGAACGGAGGCTGGAGCATCTGAAACAGGCTCAGCAGCGGCTCAGGGAGGTTG
  TCCTGGACGCACATACGACTCAGTTCAAGAGGCTGAAGGAGTTGAATGAAAGGGAGAAGAAGGAGCTTCA
  GAAGATCCTGGACAGGAAGCGCAACAACAGCATCTCAGAGGCCAAGACAAGGGAGAAACACAAGAAGGAG
  GTGGAACTGACAGAGATCAATCGGAGGCACATCACTGAGTCAGTGAACTCCATCAGGAGGCTGGAAGAGG
  CCCAGAAGCAGCGCCACGAACGCCTCTTGGCAGGGCAACAGCAGGTCCTCCAGCAGCTAGTGGAAGAGGA
  GCCCAAGCTGGTGGCCCAGCTGACCCAGGAGTGTCAGGAACAGCGAGAGAGGCTGCCCCAGGAGATCCGT
  CGGTGCCTGCTGGGCGAGACATCAGAGGGATTGGGGGATGGCCCCCTGGTGGCCTGTGCCAGCAATGGTC
  ATGCAGCTGGGAGTGGTGGGCACCAGTCCGGCGCTGACTCGGAGAGCCAGGAGGAAAACACCCAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214977 representing NM_033350
  Red=Cloning site Green=Tags(s)
  
  MAGARPGVHALQLEPPTVVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIR
  DTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAM
  NILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFSL
  EIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYE
  PNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGTSSVEMY
  RQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAETAFKTSPYPVILSFENHVDSAK
  QQAKMAEYCRSIFGEALLIDPLDKYPLSAGTPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQS
  NSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEEGLNRGPNVLMPDRDREDEE
  EDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQL
  TKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYL
  LKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDRKVGIYVEVDMFGLPVDTRRKYRTRTSQG
  NSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGRFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALL
  IYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTEMCQETPSQQQGSQLSSNPA
  PNPLDASPRWPPGPTTSPTSTSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHK
  KHQRKAVALTRRLLDGLAQARAEGKCRPSSSALSRATNVEDVKEEEKEAARQYREFQNRQVQSLLELREA
  QADAETERRLEHLKQAQQRLREVVLDAHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKE
  VELTEINRRHITESVNSIRRLEEAQKQRHERLLAGQQQVLQQLVEEEPKLVAQLTQECQEQRERLPQEIR
  RCLLGETSEGLGDGPLVACASNGHAAGSGGHQSGADSESQEENTQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033350
• ORF Size: 3708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033350.1, NP_203501.1
• RefSeq Size: 3726
• RefSeq ORF: 3711
• Locus ID: 29322
• MW: 139.7 kDa
• Gene Summary: phospholipase C isozyme activated by Gq class G proteins [RGD, Feb 2006]