Esr1 (NM_012689) Rat Tagged ORF Clone

CAT#: RR215023

Esr1 (Myc-DDK-tagged ORF) - Rat estrogen receptor 1 (Esr1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Esr1
• Synonyms: ER-alpha; Esr; RNESTROR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215023 representing NM_012689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGACCCTTCACACCAAAGCCTCGGGAATGGCCTTGTTGCACCAGATCCAAGGGAACGAGCTGG
  AGCCCCTCAATCGCCCGCAGCTCAAGATGCCCATGGAGAGGGCTCTGGGCGAGGTGTACGTGGACAACAG
  CAAGCCCGCCGTGTTCAACTACCCCGAGGGCGCCGCCTACGAGTTCAACGCCGCCGCCGCCGCCGCGGCC
  GCCGGGGCCTCGGCTCCGGTCTATGGCCAGTCGAGCATCACTTACGG[AT]CCGGGGTCCGAGGCGGCCG
  CCTTTGGTGCCAATAGTCTGGGGGCTTTCCCCCAGCTCAACAGCGTGTCGCCCAGTCCGCTGATGCTGCT
  GCACCCGCCGCCGCACGTGTCGCCGTTCCTGCACCCGCATGGCCACCAGGTGCCCTACTACCTGGAGAAC
  GAGCCCAGCGCCTACGCTGTACGCGACACCGGCCCTCCCGCCTTCTACAGGTCCAATTCTGACAATCGAC
  GCCAGAATGGCCGAGAGAGACTCTCCAGCAGCAGCGAGAAGGGAAACATGATCATGGAGTCTGCCAAGGA
  GACTCGCTACTGTGCTGTGTGTAACGACTATGCCTCTGGCTACCATTATGGGGTCTGGTCCTGTGAAGGC
  TGCAAGGCTTTCTTTAAGAGAAGCATTCAAGGACATAATGACTACATGTGTCCAGCTACAAACCAATGCA
  CCATCGATAAGAACCGGAGGAAGAGTTGCCAGGCCTGCCGGCTGCGCAAGTGTTACGAAGTGGGCATGAT
  GAAAGGCGGGATACGAAAAGACCGCCGAGGAGGGAGAATGTTGAAGCACAAGCGTCAGAGAGATGACTTG
  GAAGGCCGAAATGAAATGGGCACTTCAGGAGACATGAGAGCTGCCAACCTTTGGCCAAGTCCACTTGTGA
  TCAAGCACACTAAGAAGAATAGCCCCGCCCTGTCCTTGACAGCTGACCAGATGGTCAGTGCCTTATTGGA
  TGCTGAACCACCTTTGATCTATTCTGAATATGATCCTTCTAGACCCTTCAGTGAAGCCTCAATGATGGGC
  TTATTGACCAACCTGGCAGACAGGGAGCTGGTTCATATGATCAACTGGGCAAAGAGAGTGCCAGGCTTTG
  GGGACTTGAATCTCCACGATCAAGTTCACCTTCTGGAGTGTGCCTGGTTGGAGATCCTGATGATTGGTCT
  GGTCTGGCGCTCCATGGAACACCCGGGGAAGCTCCTGTTTGCTCCTAACTTGCTCTTGGACAGGAATCAA
  GGTAAATGTGTAGAAGGCATGGTGGAGATCTTTGACATGTTGCTGGCTACGTCAAGTCGATTCCGCATGA
  TGAACCTGCAGGGAGAAGAGTTTGTGTGCCTCAAATCAATCATTTTGCTTAATTCTGGAGTGTACACATT
  TCTATCCAGCACCTTGAAGTCTCTGGAAGAGAAGGACCACATCCACCGAGTCCTGGACAAGATCAACGAC
  ACTTTGATCCACTTGATGGCCAAAGCTGGCCTGACTCTGCAGCAACAGCATCGCCGTCTGGCCCAGCTCC
  TCCTCATCCTTTCCCATATCCGGCACATGAGTAACAAAGGCATGGAGCATCTCTACAACATGAAATGCAA
  GAATGTCGTGCCTCTCTATGACCTGCTGCTGGAGATGCTGGATGCTCATCGTCTTCATGCCCCCGCCAGT
  CGCATGGGAGTGCCCCCGGAGGAGCCTAGCCAGAGCCAGCTGACCACCACCAGCTCCACTTCAGCACATT
  CCTTACAAACCTACTACATCCCCCCGGAAGCAGAGGGCTTCCCCAACACCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215023 representing NM_012689
  Red=Cloning site Green=Tags(s)
  
  MTMTLHTKASGMALLHQIQGNELEPLNRPQLKMPMERALGEVYVDNSKPAVFNYPEGAAYEFNAAAAAAA
  AGASAPVYGQSSITYXXPGSEAAAFGANSLGAFPQLNSVSPSPLMLLHPPPHVSPFLHPHGHQVPYYLEN
  EPSAYAVRDTGPPAFYRSNSDNRRQNGRERLSSSSEKGNMIMESAKETRYCAVCNDYASGYHYGVWSCEG
  CKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDL
  EGRNEMGTSGDMRAANLWPSPLVIKHTKKNSPALSLTADQMVSALLDAEPPLIYSEYDPSRPFSEASMMG
  LLTNLADRELVHMINWAKRVPGFGDLNLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQ
  GKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKIND
  TLIHLMAKAGLTLQQQHRRLAQLLLILSHIRHMSNKGMEHLYNMKCKNVVPLYDLLLEMLDAHRLHAPAS
  RMGVPPEEPSQSQLTTTSSTSAHSLQTYYIPPEAEGFPNTI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012689
• ORF Size: 1800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012689.1, NP_036821.1
• RefSeq Size: 2090
• RefSeq ORF: 1803
• Locus ID: 24890
• MW: 67 kDa
• Gene Summary: acts as a transcriptional activator when bound to estrogen; may play a role in myocardial regulation [RGD, Feb 2006]