Dbh (NM_013158) Rat Tagged ORF Clone

CAT#: RR215031

Dbh (Myc-DDK-tagged ORF) - Rat dopamine beta-hydroxylase (dopamine beta-monooxygenase) (Dbh), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dbh
• Synonyms: DOPBHY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215031 representing NM_013158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCTCACCTCAGCCACCAGCCTTGCTGGAGCCTCCCCAGCCCCAGCGTCCGTGAGGCGGCTTCCA
  TGTATGGCACTGCTGTGGCCATCTTCCTGGTCATCCTGGTGGCTGCACTGCAGGGCTCGGAGCCTCCGGA
  GAGCCCCTTCCCTTACCACATCCCCCTGGACCCTGAAGGGACTTTAGAGCTCTCGTGGAACGTCAGCTAT
  GACCAGGAGATCATCCACTTCCAGCTCCAGGTGCAAGGGCCGAGGGCTGGGGTCCTGTTCGGAATGTCGG
  ATCGAGGTGAGATGGAGAACGCAGACCTCGTCATGCTCTGGACTGACGGGGACAGGACCTACTTTGCGGA
  CGCCTGGAGTGACCAGAAAGGGCAGATCCATCTGGATACCCATCAGGACTACCAGCTGCTCCAGGCACAG
  AGGGTGTCAAACAGCCTATCCCTGCTCTTCAAGAGGCCCTTTGTCACCTGCGACCCCAAGGATTATGTCA
  TTGAGGATGACACTGTCCATCTAGTGTATGGGATCCTGGAGGAGCCATTCCAGTCCCTGGAGGCCATCAA
  CACCTCAGGCCTACATACGGGACTACAGCAGGTGCAGCTTCTGAAGCCTGAGGTCTCCACTCCAGCCATG
  CCTGCGGATGTACAAACCATGGATATCCGGGCTCCTGACGTCCTCATCCCCAGCACTGAGACCACATACT
  GGTGCTATATCACTGAGCTACCCCTACACTTCCCCCGACACCACATCATCATGTATGAGGCCATTGTCAC
  GGAGGGCAATGAGGCCCTGGTGCACCACATGGAGGTCTTCCAATGTACAAATGAGTCTGAGGCCTTCCCC
  ATGTTCAACGGACCCTGCGACTCCAAGATGAAACCTGACAGACTCAACTACTGTCGCCACGTGCTGGCGG
  CATGGGCCCTGGGCGCCAAGGCATTTTACTACCCAGAAGAAGCCGGTGTCCCTTTGGGGAGCTCAGGATC
  CTCCCGGTTTCTCCGACTGGAAGTTCATTACCACAATCCACGGAATATACAAGGCCGGCGCGACTCCTCT
  GGCATCCGTCTACACTACACAGCTAGTCTCCGACCCAATGAGGCAGGCATCATGGAGCTTGGACTGGTGT
  ACACGCCCTTGATGGCCATCCCCCCTCAGGAGACCACCTTTGTTTTGACTGGCTACTGCACAGACAGGTG
  CACCCAGATGGCACTGCCGAAATCTGGAATCCGCATCTTTGCCTCACAGCTCCACACGCACCTGACCGGC
  AGGAAGGTGATTACTGTGCTCGCCAGGGATGGCCAACAGAGGGAAGTGGTGAACAGAGACAACCACTACA
  GCCCCCACTTTCAGGAGATCAGAATGCTGAAGAATGCTGTGACTGTCCACCAGGGGGATGTCCTCATCAC
  TTCGTGCACATACAACACAGAAAACAGGACAATGGCCACAGTGGGGGGGTTTGGAATCTTGGAGGAGATG
  TGCGTCAACTACGTCCACTACTACCCCAAAACAGAGCTGGAGCTCTGCAAGAGTGCCGTGGATGATGGCT
  TCCTGCAGAAATACTTCCACATAGTAAACCGGTTCGGCAATGAGGAGGTCTGCACCTGCCCTCAGGCCTC
  TGTCCCCCAGCAGTTCGCCTCTGTGCCCTGGAACTCTTTCAATCGTGATATGCTCAAGGCTTTGTATAAC
  TATGCCCCTATCTCTGTGCACTGTAACAAGACCTCTGCCGTCCGCTTCCCGGGTAACTGGAACCTGCAGC
  CTCTGCCTAAGATCACTTCCGCAGTGGAAGAACCCGACCCACGCTGCCCCATCCGACAGACTCGGGGACC
  CGCCGGCCCCTTCGTTGTCATCACCACGGAGGCAGACACTGAGCGCCGGCCCCTTCGTTGTCATCACCAC
  GGAGGCAGACACTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215031 representing NM_013158
  Red=Cloning site Green=Tags(s)
  
  MQPHLSHQPCWSLPSPSVREAASMYGTAVAIFLVILVAALQGSEPPESPFPYHIPLDPEGTLELSWNVSY
  DQEIIHFQLQVQGPRAGVLFGMSDRGEMENADLVMLWTDGDRTYFADAWSDQKGQIHLDTHQDYQLLQAQ
  RVSNSLSLLFKRPFVTCDPKDYVIEDDTVHLVYGILEEPFQSLEAINTSGLHTGLQQVQLLKPEVSTPAM
  PADVQTMDIRAPDVLIPSTETTYWCYITELPLHFPRHHIIMYEAIVTEGNEALVHHMEVFQCTNESEAFP
  MFNGPCDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGVPLGSSGSSRFLRLEVHYHNPRNIQGRRDSS
  GIRLHYTASLRPNEAGIMELGLVYTPLMAIPPQETTFVLTGYCTDRCTQMALPKSGIRIFASQLHTHLTG
  RKVITVLARDGQQREVVNRDNHYSPHFQEIRMLKNAVTVHQGDVLITSCTYNTENRTMATVGGFGILEEM
  CVNYVHYYPKTELELCKSAVDDGFLQKYFHIVNRFGNEEVCTCPQASVPQQFASVPWNSFNRDMLKALYN
  YAPISVHCNKTSAVRFPGNWNLQPLPKITSAVEEPDPRCPIRQTRGPAGPFVVITTEADTERRPLRCHHH
  GGRH*
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013158
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 2250
• RefSeq ORF: 1866
• Locus ID: 25699
• MW: 70 kDa
• Gene Summary: oxidoreductase that catalyzes the conversion of 3,4 dihydroxyphenethylamine, ascorbate, and O(2) to noradrenaline, dehydroascorbate, and H(2) in norephinephrine biosynthesis [RGD, Feb 2006]