Gak (NM_031030) Rat Tagged ORF Clone

CAT#: RR215048

Gak (Myc-DDK-tagged ORF) - Rat cyclin G associated kinase (Gak), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gak
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215048 representing NM_031030
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTGCTGCAGTCTGCACTGGACTTCCTGGCGGGCCCTGGTTCTCTTGGCGGAGCTGCCGGCCGTG
  ACCAGAGTGACTTCGTGGGGCAGACTGTGGAGCTGGGCGAGCTGCGTCTGCGGGTGCGGCGGGTCCTGGC
  CGAGGGAGGGTTTGCATTTGTTTATGAAGCTCAAGATCTGGGAAGTGGCAGAGAGTATGCATTAAAGAGA
  TTACTATCCAATGAAGAGGAAAAGAACAGAGCCATCATTCAGGAAGTATGTTTCTTGAAAAAACTTTCTG
  GCCACCCCAATATTGTCCAGTTCTGCTCTGCAGCATCCATAGGAAAAGAGGAATCGGACACTGGGCAGGC
  TGAGTTCCTCCTGCTTACGGAGCTCTGTAAAGGACAGCTGGTGGAGTTTCTCAGGAGAGTTGAATGTAAA
  GGCCCTCTATCCTGCGACAGCATTCTGAAGATCTTCTACCAGACATGCAGAGCAGTGCAGCACATGCACA
  GGCAGAAACCACCCATCATCCACAGGGATCTCAAGGTTGAAAACTTACTGCTTAGTAACCAGGGGACCAT
  TAAGCTGTGTGACTTTGGCAGTGCCACAACCATCTCCCATTATCCTGACTACAGCTGGAGCGCCCAGAAG
  CGAGCAATGGTGGAGGAAGAGATCACGAGGAACACCACACCCATGTACAGAACGCCAGAAATTGTAGACC
  TGTATTCTAACTTCCCTATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTATCTTATACCTGCTGTG
  TTTCCGGCAGCATCCTTTTGAAGATGGAGCAAAACTTCGGATAGTCAATGGGAAGTATTCCATTCCTGTG
  AATGACACTCGTTACACAGTCTTCCATGACCTTATTCGTGGCATGCTAAAGGTCAATCCAGAAGAGAGGC
  TATCCATTGCTGAAGTTGTCCGACAACTGCAGGAAATTGCAGCAGCCCGGAATGTGAACCCCAAAGCCCC
  CATCACAGAACTTCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGAGCACAACCACCTTCT
  GGGGGCCCTGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCTATGGTGGGTTTCTCG
  ATATCCTACGGGGTGGGACAGAACGGCTCTTCACCAACCTCAAGGATACTTCCTCCAAGGTCATCCAGTC
  TGTGGCTAACTATGCAAAGGGCGATCTTGACATATCTTACATCACATCCAGGATTGCAGTGATGTCGTTC
  CCAGCAGAAGGTGTGGAGTCAGCAATCAAAAACAATATAGAGGATGTACGATTGTTTCTGGATGCCAAGC
  ATCCAGGACATTATGCTGTCTACAACCTTTCTCCAAGAATATACCGGGCTTCCAAGTTCCACAATCGGGT
  CACTGAGTGTGGCTGGGCAGTCAGGCGGGCACCACATCTCCACAGTTTGTATACTCTATGCAGGAGCATG
  CATGCCTGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTACATTGCATGGATGGGAGAGCTGCGTCTG
  CTGTGGCAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGCTGCTGTGTACATGTT
  CAGCATGAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAATACGTGTGTGACATG
  GTGGCAGAGGAACCCATCACGCCCCACAGCAAGCCAATGCTGGTGAAATCTGTTGTCATGACCCCTGTGC
  CACTGTTCAGCAAGCAGAGGAATGGCTGCCGACCATTCTGTGAGGTCTATGTTGGGGAGGAGCGTGTAAC
  CACCACATCCCAGGAGTATGACAGGATGAAGGAATTTAAAATTGAGGATGGCAAGGCTGTCATCCCCCTG
  GGCATAACAGTTCAAGGCGATGTCCTCACCATCATTTACCATGCCAGATCCACTTTAGGAGGGAGACTGC
  AGGCCAAGATGGCGTCCATGAAAATGTTCCAGATTCAGTTCCACACTGGGTTCGTGCCTCGAAACGCAAC
  CACTGTGAAATTTGCAAAGTATGACCTGGATGCTTGTGACATTCAAGAGAAGTACCCAGATCTGTTCCAG
  GTGAACCTGGAAGTGGAGGTGGAGCCTAGAGACAGGCCCAGCCGAGACGTTCCACCTTGGGAGAATACCA
  GCCTAAGGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAACAGGACATTCTGTCTAAGTT
  TGGGAAACCAGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAGGCAGGGTCTCCAGAG
  GCTGAGATCACAGAGTCGGACTCACCACAGAGCAGCAGCACGGACACCAACCACTTTCTTCACACACTGG
  ATTGGCAGGAGGAAAAAGACCCAGAGACTGGTGTAGACAATACCTCTCCTAAGGAGAGTCAGTCTAACCT
  GATTGCAGATGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATGCCCTAGTGAAGAGAGAAAG
  CCTGGGGCTGGAGAAGATACACCAAGGCTGGCAGCTGGGACCAGACAGCAAGACTTAATATTTGATGTGG
  GCATGCTGGCTGCCCCACAGGAGCCTGTACAGCCTGAAGAAGGTGTCGATCTCCTGGGGCTGCACTCTGA
  AGGGGACTTAAGGCCTGCTGCTCCCTTGCAGGCTAGCGGGGTCCAGTCTAGCAACACTGACCTGTTGAGC
  TCCCTTCTTGAACCATCTGATGCTTCTCAAGTGGGACCTCCTGGTGACCTGCTTGGTGGTGAGACTCCTC
  TGCTGTTAGCAAGCCCAGTTTCTCTTCTTGGGGTGCAGAGCAACCTGCAAGGAAAAGTCCCTGACACTGT
  GGACCCATTTGACCAGTTCCTGCTGCCATCCAGCTCGGACACCCAGCCCTGCTCCAAGCCTGATCTCTTT
  GGAGAGTTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCGACCCACAGCGCCCCACCCC
  CATCCTGCAGCACTGCCTTCCTGCACCTGGGAGATCTGCCAGCAGAGCCCAACAAGGTGATTGCTTCATC
  CAGCCACCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGAGACTGCTCTACCTGGGCCGGCCTCCATG
  CCAGTACCAGAAGGTACTCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCAGGCCCCAACCCCAGCCAAA
  CCAAGTCTCAGAACCCAGACCCATTTGCTGACCTCAGTGATCTCAGCTCTAGCCTCCAAGGCTTGCCTGC
  TGGACTTCCTGCAGGGAGCTTCGTTGGCACATCAGCCACCACTCACAAAAGCAACAGCTCCTGGCAGACA
  ACTCGTCCCACAGCCCCTGGAACCTCATGGCCCCCGCAGGCCAAGCCAGCCCCCAGAGCCTCTGAACAAC
  TAAGGTCTCACTTTAGTGTGATTGGGGCCCGAGAAGAGAGAGGTGTCCGTGCGCCCAGCTTTGCCCAAAA
  GCCAAAGGTCTCAGAAAATGATTTTGAAGATCTGCTGCCTAATCAAGGCTTCTCTAAGTCTGACAAGAAG
  GGGCCAAAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACCCATTCAAATTGAAGC
  TTTTGGACTGGATTGAAGGCAAAGAGAGGAATATTCGTGCACTGCTGTCCACTCTGCACACAGTATTGTG
  GGATGGGGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAGCAGGTGAAGAAGCAG
  TACCGCCGTGCAGTGCTGGTAGTGCATCCTGATAAGGCCACAGGGCAGCCATATGAACAGTCTGCCAAGA
  TGATCTTCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCAAGGCCCCTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215048 representing NM_031030
  Red=Cloning site Green=Tags(s)
  
  MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKR
  LLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLRRVECK
  GPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQK
  RAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPV
  NDTRYTVFHDLIRGMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPS
  GGPVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSF
  PAEGVESAIKNNIEDVRLFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSM
  HAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDM
  VAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPL
  GITVQGDVLTIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQ
  VNLEVEVEPRDRPSRDVPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPE
  AEITESDSPQSSSTDTNHFLHTLDWQEEKDPETGVDNTSPKESQSNLIADGDGSEVSDEEEASCPSEERK
  PGAGEDTPRLAAGTRQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQASGVQSSNTDLLS
  SLLEPSDASQVGPPGDLLGGETPLLLASPVSLLGVQSNLQGKVPDTVDPFDQFLLPSSSDTQPCSKPDLF
  GEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPNKVIASSSHPDLLGGWDTWAETALPGPASM
  PVPEGTLFSSAGHPAPPGPNPSQTKSQNPDPFADLSDLSSSLQGLPAGLPAGSFVGTSATTHKSNSSWQT
  TRPTAPGTSWPPQAKPAPRASEQLRSHFSVIGAREERGVRAPSFAQKPKVSENDFEDLLPNQGFSKSDKK
  GPKTMAEMRKQELARDTDPFKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQ
  YRRAVLVVHPDKATGQPYEQSAKMIFMELNDAWSEFENQGSRPLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031030
• ORF Size: 3915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031030.1, NM_031030.2, NP_112292.1
• RefSeq Size: 4453
• RefSeq ORF: 3918
• Locus ID: 81659
• MW: 143.7 kDa
• Gene Summary: may be a serine/threonine protein kinase; may be involved with nucleotide metabolism [RGD, Feb 2006]