Ppp3ca (NM_017041) Rat Tagged ORF Clone

CAT#: RR215113

Ppp3ca (Myc-DDK-tagged ORF) - Rat protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (Ppp3ca), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp3ca
• Synonyms: Calna1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215113 representing NM_017041
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGCCCAAGGCGATTGATCCCAAGTTGTCGACTACGGACAGGGTGGTGAAAGCCGTTCCATTTC
  CGCCAAGTCACCGGCTGACAGCAAAGGAAGTGTTTGATAACGATGGGAAGCCTCGTGTGGATATCTTAAA
  AGCACATCTCATGAAGGAAGGCAGGCTGGAAGAAAGTGTCGCGTTGAGAATAATAACAGAGGGTGCTTCG
  ATTCTCCGACAGGAAAAAAACTTGCTGGATATTGATGCCCCAGTCACAGTTTGCGGGGACATCCATGGAC
  AATTCTTTGACTTGATGAAGCTCTTTGAAGTGGGAGGATCTCCTGCCAACACTCGCTACCTCTTCTTAGG
  GGACTATGTTGACAGAGGGTACTTCAGTATCGAATGTGTGCTGTATTTGTGGGCCTTGAAAATTCTTTAC
  CCCAAAACACTGTTTTTACTTCGTGGAAACCATGAATGTAGGCACCTAACAGAGTATTTCACGTTTAAAC
  AAGAATGTAAAATAAAGTATTCAGAACGCGTTTATGACGCCTGTATGGATGCCTTCGACTGCCTTCCCCT
  GGCTGCGCTGATGAACCAACAATTCCTGTGTGTACACGGTGGTTTGTCTCCAGAGATTAACACTCTAGAT
  GACATCAGAAAATTAGACCGATTCAAAGAACCACCTGCTTATGGGCCTATGTGTGACATCTTGTGGTCAG
  ACCCCCTGGAGGACTTTGGAAATGAGAAGACTCAGGAACATTTCACTCACAACACAGTCAGGGGTTGTTC
  GTACTTCTACAGTTACCCGGCTGTATGTGACTTCCTGCAGCACAATAATTTGTTGTCCATACTCCGAGCC
  CACGAAGCCCAGGACGCAGGGTACCGCATGTACAGGAAAAGCCAAACAACTGGCTTCCCGTCTCTAATTA
  CGATCTTCTCGGCACCAAATTACTTAGATGTGTACAATAATAAAGCTGCAGTGTTGAAGTACGAGAACAA
  CGTGATGAACATCAGGCAGTTCAACTGCTCCCCCCATCCGTACTGGCTCCCAAATTTCATGGATGTTTTC
  ACCTGGTCGCTGCCATTTGTTGGGGAGAAAGTGACTGAGATGCTGGTAAACGTCCTGAACATCTGCTCAG
  ATGATGAACTGGGGTCAGAAGAAGATGGATTTGACGGAGCCACGGCTGCAGCCCGGAAGGAGGTCATCAG
  GAACAAGATCCGAGCAATAGGCAAAATGGCCAGAGTATTCTCAGTTCTCAGAGAAGAGAGTGAGAGCGTT
  CTAACTCTGAAGGGCCTGACCCCGACTGGCATGCTCCCCAGCGGAGTGCTCTCTGGCGGGAAACAAACTC
  TGCAAAGCGCTACTGTTGAGGCCATTGAGGCTGATGAAGCCATCAAAGGATTCTCACCACAACATAAGAT
  TACCAGCTTCGAGGAGGCCAAGGGCTTAGACCGAATTAACGAGAGGATGCCGCCTCGCAGAGACGCCATG
  CCTTCCGACGCCAACCTTAACTCCATCAACAAGGCTCTCGCCTCAGAGACTAACGGCACAGACAGCAACG
  GCAGTAATAGCAGCAATATTCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215113 representing NM_017041
  Red=Cloning site Green=Tags(s)
  
  MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGAS
  ILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILY
  PKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLD
  DIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCDFLQHNNLLSILRA
  HEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVF
  TWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESV
  LTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAM
  PSDANLNSINKALASETNGTDSNGSNSSNIQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017041
• ORF Size: 1563 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017041.1, NP_058737.1
• RefSeq Size: 2337
• RefSeq ORF: 1566
• Locus ID: 24674
• MW: 58.6 kDa
• Gene Summary: catalytic subunit of Calcineurin (CaN), ubiquitously expressed Ca2+/CaM-dependent protein phosphatase; mediates activities of transcription factors and ion channels; involved in regulation of T-cell activation [RGD, Feb 2006]