Cox15 (NM_001033699) Rat Tagged ORF Clone

CAT#: RR215404

Cox15 (Myc-DDK-tagged ORF) - Rat COX15 homolog, cytochrome c oxidase assembly protein (yeast) (Cox15), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cox15
• Synonyms: MGC124864
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215404 representing NM_001033699
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACGGTTGTTCCTCCCGCCCCTCAAGGCCTTGATGGGGAGCCGCAATGTCGGGCTCCTGGTCCCTA
  AGGCGGCATCTAGAGCACAGTGTGGCGGCGGCAGCTATGGCGGCAGGCGTCCTCTGAGGCCAGGGCAGTA
  CGGCACCATCACTGAAGTGGCTTTGCAATCTGGAAAAGGCACAGTGTCCCTTCCTTCGAGGGCAGCCGAG
  CGGGCAGTGGGCCGATGGCTCCTGGTGTGCAGTGGAACAGTGGCTGGAGCAGTTATTCTTGGAGGAGTGA
  CCAGGCTGACGGAGTCTGGCCTCTCAATGGTAGACTGGCATTTAATAAAGGAGATGAAGCCACCCACAAG
  CCAGGAAGAATGGGAAGCGGAGTTCCAAAAGTACCAGCAATTTCCGGAATTTAAAATCCTGAATCATGAC
  ATGACACTGGCGGAATTCAAGTTTATCTGGTACATGGAATACTCACACCGGATGTGGGGTCGAGCTGTAG
  GCCTTGCATACATCCTGCCTGCTGCCTACTTTTGGAGAAAGGGTTGGCTCAACCGTGGCATGAAAGGACG
  TGTTCTTGCCCTCTGTGGCTTAGTCTGTTTCCAGGGTCTGTTGGGTTGGTATATGGTGAAAAGTGGATTA
  GAAGAAAAACCAGAGTCCTATGACATCCCTCGGGTCAGTCAGTACCGCCTCGCAGCCCACCTGGGGTCAG
  CACTGATTCTCTACTGTGGCAGCCTGTGGACCTCCCTGTCACTGCTACTGCCTCAGCACAAGATACCGGA
  AACCCGACAGCTCCTGTGGCTGAGACGCTTTGCTGGTGGAACAGCAGGCCTGGTGTTCCTCACAGCTCTC
  TCAGGGGCTTTTGTGGCAGGGCTGGATGCTGGGCTCGTTTACAACTCCTTCCCCAAAATGGGAGAATCTT
  GGATCCCAGAGGACCTCCTGACCTTCTCTCCCATCCTGAAGAATGTTTTTGAGAATCCTACTATGGTTCA
  GTTTGATCACCGGCTTCTGGGAGTCACGTCTGTCACTGCCATTACAATGCTCTATTTCCTGTCCCGGAGA
  ATCCCCCTTCCTCGAAGAACCAAGATGGCAGCGGTGACTCTGCTAGCTTTGGCATATGCACAGGTGGCCT
  TGGGCATCAGCACTCTGCTTATGTATGTGCCAACTCCGCTGGCTGCCACACACCAGTCAGGCTCCTTGGC
  TCTGCTCAGTGGTGCCCTTTGGCTCATGAATGAACTCCGAAGAGTTCCAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215404 representing NM_001033699
  Red=Cloning site Green=Tags(s)
  
  MQRLFLPPLKALMGSRNVGLLVPKAASRAQCGGGSYGGRRPLRPGQYGTITEVALQSGKGTVSLPSRAAE
  RAVGRWLLVCSGTVAGAVILGGVTRLTESGLSMVDWHLIKEMKPPTSQEEWEAEFQKYQQFPEFKILNHD
  MTLAEFKFIWYMEYSHRMWGRAVGLAYILPAAYFWRKGWLNRGMKGRVLALCGLVCFQGLLGWYMVKSGL
  EEKPESYDIPRVSQYRLAAHLGSALILYCGSLWTSLSLLLPQHKIPETRQLLWLRRFAGGTAGLVFLTAL
  SGAFVAGLDAGLVYNSFPKMGESWIPEDLLTFSPILKNVFENPTMVQFDHRLLGVTSVTAITMLYFLSRR
  IPLPRRTKMAAVTLLALAYAQVALGISTLLMYVPTPLAATHQSGSLALLSGALWLMNELRRVPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033699
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033699.1, NM_001033699.2, NM_001033699.3, NM_001033699.4, NP_001028871.2
• RefSeq Size: 3007 bp
• RefSeq ORF: 1245 bp
• Locus ID: 309391
• Cytogenetics: 1q54
• MW: 45.9 kDa