Asah1 (NM_053407) Rat Tagged ORF Clone

CAT#: RR215418

Asah1 (Myc-DDK-tagged ORF) - Rat N-acylsphingosine amidohydrolase (acid ceramidase) 1 (Asah1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asah1
• Synonyms: Asah
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215418 representing NM_053407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCCGTAGTCTCCTCACCTGGGTCCTGGCCGCGGCTGTCACCTGCGCCCAGGCACAGCAAGTGC
  CACCGTGGACAGAAGATTGCAGAAAATCAACTTATCCTCCTTCTGGACCAACCTATAGAGGACCAGTTCC
  GTGGTACACCATAAATCTTGATTTACCACCCTACAAGAGATGGCATGAATTATTGGCTCACAAGGCACCT
  GTGTTGAGAACTTTAGTGAATTCCATCTCGAATTTAGTGAATGCATTTGTGCCAAGTGGAAAAATAATGC
  AGATGGTGGATGAAAAGTTGCCTGGTCTGATTGGCAGCATTCCTGGCCCTTTTGGAGAGGAAATGAGGGG
  GATTGCAGATGTTACTGGGATTCCTCTAGGAGAGATTATTTCATTCAACATTTTCTATGAACTGTTCACC
  ATGTGTACATCGATCATAACTGAAGATGGAAAAGGTCATTTACTACATGGAAGAAACATGGATTTTGGAA
  TATTTCTTGGGTGGAACATTAACAACAACACTTGGGTGGTGACAGAAGAATTAAAGCCTTTAACAGTGAA
  TTTGGACTTCCAGAGGAACAATAAGACTGTGTTCAAGGCTACAAGTTTCGCTGGATACGTGGGCATGTTG
  ACAGGATTCAAACCAGGACTGTTAAGTCTTACACTGAATGAACGTTTCAGTTTAAATGGTGGTTATCTGG
  GTATCCTAGAATGGATGTTTGGAAAGAAAAATGCCCAATGGGTAGGGTTTATCACTAGATCAGTTCTGGA
  AAATAGCACAAGTTATGAAGAAGCCAAGAATATATTGACCAAGACCAAGATAACGGCCCCAGCATATTTT
  ATCCTGGGAGGCAACCAGTCTGGAGAAGGTTGTGTGATTACACGAGAAAGAAAAGAGTCTTTAGACGTCT
  ATGAACTTGATCCTAAGCATGGCAGATGGTACGTGGTACAAACCAATTATGACCGGTGGAAAAACACCTT
  GTTTCTTGATGACCGCAGAACACCTGCGAAGAAGTGTCTAAATCACACGACACAGAAGAATCTGTCATTT
  GCTACCATCTATGATGTTCTATCAACAAAACCTGTCCTCAACAAGCTGACTGTATTCACAACCTTGATAG
  ATGTCACCAAAGATCAATTTGAAAGCCACCTTCGAGATTGCCCAGACCCTTGTATAGGCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215418 representing NM_053407
  Red=Cloning site Green=Tags(s)
  
  MLGRSLLTWVLAAAVTCAQAQQVPPWTEDCRKSTYPPSGPTYRGPVPWYTINLDLPPYKRWHELLAHKAP
  VLRTLVNSISNLVNAFVPSGKIMQMVDEKLPGLIGSIPGPFGEEMRGIADVTGIPLGEIISFNIFYELFT
  MCTSIITEDGKGHLLHGRNMDFGIFLGWNINNNTWVVTEELKPLTVNLDFQRNNKTVFKATSFAGYVGML
  TGFKPGLLSLTLNERFSLNGGYLGILEWMFGKKNAQWVGFITRSVLENSTSYEEAKNILTKTKITAPAYF
  ILGGNQSGEGCVITRERKESLDVYELDPKHGRWYVVQTNYDRWKNTLFLDDRRTPAKKCLNHTTQKNLSF
  ATIYDVLSTKPVLNKLTVFTTLIDVTKDQFESHLRDCPDPCIGW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053407
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053407.1, NM_053407.2, NM_053407.3, NP_445859.2
• RefSeq Size: 2436
• RefSeq ORF: 1185
• Locus ID: 84431
• MW: 44.4 kDa