Ogdh (NM_001017461) Rat Tagged ORF Clone

CAT#: RR215419

Ogdh (Myc-DDK-tagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ogdh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215419 representing NM_001017461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT
  CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA
  ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA
  AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC
  AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCTCATGCACAGTCCCTGGTGGAAGCACA
  GCCTAACGTCGACAAGCTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCGTATCAGATACGA
  GGGCACCATGTAGCACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTG
  ACATTATCTCATCCACAGACAAACTTGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCA
  CTTACCCACCACCACTTTCATCGGGGGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTG
  GAGATGGCCTACTGCCAGCACATTGGTGTGGAGTTCATGTTCATTAACGATTTGGAGCAATGCCAGTGGA
  TCCGGCAGAAGTTTGAGACCCCTGGGATCATGCAGTTCACCAATGAGGAGAAGCGGACCCTGCTGGCCAG
  GCTTGTACGGTCCACCAGGTTTGAGGAGTTCCTGCAGCGAAAGTGGTCCTCTGAGAAGCGTTTTGGTCTG
  GAAGGCTGTGAGGTGCTGATCCCTGCCCTCAAGACAATCATTGACATGTCAAGTGCAAATGGAGTGGACT
  ATGTGATCATGGGGATGCCACACAGAGGACGACTGAACGTGCTAGCCAATGTCATCAGGAAGGAGCTGGA
  GCAGATATTCTGTCAGTTTGACTCGAAGCTGGAGGCAGCTGATGAGGGTTCCGGGGACATGAAGTACCAC
  CTGGGCATGTATCACCGAAGGATCAACCGTGTGACCGACAGAAACATCACTCTGTCCTTGGTGGCTAACC
  CTTCCCACTTAGAGGCTGCTGACCCTGTAGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGA
  CACTGAAGGGAAAAAGGTGATGTCTATCCTGCTGCATGGGGATGCTGCCTTTGCTGGCCAGGGTATCGTG
  TATGAGACCTTCCATCTCAGCGACCTGCCATCCTATACAACCCACGGCACCGTTCACGTGGTTGTCAACA
  ACCAGATTGGCTTCACCACAGACCCTCGGATGGCCCGCTCTTCTCCCTACCCCACTGATGTGGCCCGAGT
  GGTGAACGCCCCCATTTTCCATGTCAACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCA
  GCTGAGTGGAGAAACACCTTCCACAAGGATGTTGTTGTTGATCTGGTGTGTTATCGACGAAATGGCCACA
  ACGAGATGGATGAACCTATGTTCACACAGCCACTCATGTACAAGCAGATCCGTAAGCAGAAGCCGGTACT
  GCAGAAGTATGCAGAATTGCTGGTATCCCAGGGGGTCGTCAACCAGCCCGAATATGAGGAGGAAATCTCC
  AAGTACGATAAGATCTGTGAGGAAGCATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACT
  GGCTGGATTCCCCCTGGCCTGGCTTTTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCAC
  TGGCCTGGAGGAGGACATCTTGACCCACATTGGGAATGTGGCCAGTTCTGTACCTGTGGAGAACTTTACC
  ATCCATGGAGGGCTGAGCCGGATCTTGAAGACTCGCAGAGAGCTTGTGACAAACCGGACTGTGGACTGGG
  CCCTGGCAGAGTACATGGCGTTCGGATCACTCCTGAAGGAAGGCATCCATGTCCGGCTGAGTGGCCAGGA
  CGTGGAGCGGGGTACCTTCAGCCATCGCCACCATGTGCTCCATGACCAGAATGTGGACAAAAGAACCTGC
  ATCCCCATGAACCACCTTTGGCCTAACCAGGCCCCTTACACCGTGTGCAACAGCTCGTTGTCTGAGTATG
  GTGTCCTGGGCTTTGAACTGGGCTTTGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTT
  TGGTGATTTCAACAACATGGCACAGTGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTG
  CGGCAGAATGGCATCGTGCTCCTGCTACCTCACGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCC
  GTCCAGAGAGGTTTCTGCAGATGTGCAATGATGACCCAGATGTCCTGCCTAACCTGCAGGAGGAAAACTT
  TGACATCAGTCAGCTCTACGACTGCAACTGGATTGTCGTCAACTGCTCCACCCCTGGCAACTTCTTCCAC
  GTGCTGCGACGACAGATCTTGCTGCCCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGC
  GCCACCCTGAGGCAAGAACTAGCTTTGATGAGATGCTGCCAGGAACCCACTTCCAGCGTGTGATCCCAGA
  AGATGGACCTGCAGCTCAGAACCCAGACAAAGTCAAGAGGCTTCTCTTCTGCACTGGCAAGGTGTACTAT
  GACCTCACCCGAGAGCGCAAAGCCAGGGACATGGCAGAGGAGGTGGCTATTACAAGGATTGAGCAGCTGT
  CACCATTCCCCTTCGACCTCCTGCTGAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCA
  GGAAGAGCACAAGAATCAAGGCTACTATGACTATGTCAAGCCAAGACTTCGGACCACCATTGACCGTGCT
  AAGCCTGTCTGGTATGCTGGCCGAGACCCAGCAGCCGCTCCAGCCACTGGCAACAAGAAGACCCACCTGA
  CAGAGCTGCAGCGCTTTCTGGACACAGCCTTTGACCTGGACGCCTTCAAGAAATTCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215419 representing NM_001017461
  Red=Cloning site Green=Tags(s)
  
  MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPK
  SVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRSSLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIR
  GHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRL
  EMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGL
  EGCEVLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDMKYH
  LGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIV
  YETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVA
  AEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEIS
  KYDKICEEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDILTHIGNVASSVPVENFT
  IHGGLSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRTC
  IPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWV
  RQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPNLQEENFDISQLYDCNWIVVNCSTPGNFFH
  VLRRQILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPEDGPAAQNPDKVKRLLFCTGKVYY
  DLTRERKARDMAEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRA
  KPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017461
• ORF Size: 3069 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017461.1, NP_001017461.1
• RefSeq Size: 3266 bp
• RefSeq ORF: 3072 bp
• Locus ID: 360975
• Cytogenetics: 14q21
• MW: 116.3 kDa