Plod1 (NM_053827) Rat Tagged ORF Clone

CAT#: RR215422

Plod1 (Myc-DDK-tagged ORF) - Rat procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (Plod1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plod1
• Synonyms: Plod
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215422 representing NM_053827
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTCCCTGCTGCTCCTGGCCTCGCTGGCCTGGCTTCTTCTGGCCCAGGCGAAGGACGACGCCAAGC
  TGGAGGACAACCTTTTGGTCCTCACCGTGGCCACAAAAGAGACAGAGGGCTTCCGCCGATTCAAGCGCTC
  AGCCCAGTTCTTCAACTACAAGATCCAGTCACTGGGCCTGGGGGAGGACTGGAGCGCGGCCGGGGGACCA
  TCGGCAGCAGGTGGAGGACAGAAGGTTCGGCTGCTGAAGAAGGCACTGAAAAAGTATGCAGACAAGGAAG
  ACCTGGTTATTCTCTTCGTTGACAGCTATGACGTGGTGTTTGCCTCGGGACCCCGGGAACTCTTAAAGAA
  GTTCCAGCAGGCCAAGAGCCGGGTGGTCTTCTCAGCAGAGGAGCTTATCTACCCTGACCGGAGGCTGGAG
  GCCAAGTATCCCACAGTCCCCGATGGCAAACGATTCTTGGGCTCTGGAGGCTTCATTGGTTATGCTCCCA
  GCCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAATGACAGCGACCAGCTCTTTTATACCAAGAT
  CTTCTTGGACCCGGAGAAGAGGGAGCAAATCAATATCAGCCTGGACCATCGCTGCCGAATCTTCCAGAAC
  CTGGATGGAGCCTTGGATGAAGTCGTACTCAAGTTTGAAATGGGCCACGTGAGAGCCCGGAATCTGGCCT
  ATGACACCCTCCCAGTGGTCATCCACGGCAACGGGCCCACCAAGCTGCAGGTGAACTACCTGGGCAACTA
  CATTCCTCGATTCTGGACCTTCGAAACGGGCTGCACCGTGTGCGACGAGGGCCTGCGAAGCCTCAAGGGC
  ATTGGGGATGAAGCTCTCCCTACCGTCCTGGTCGGTGTGTTCATTGAGCAGCCCACACCATTCCTATCCT
  TGTTCTTTCGGCGGCTGCTGCACCTACGATACCCTCAGAAACAGATGCGGCTCTTCATTCACAACCAAGA
  ACAGCACCACAAGCTTCAGGTAGAGCAGTTCCTGGCAGAGCACGGCGGTGAGTACCAGTCTGTGAAGCTA
  GTGGGTCCCGAGGTACGCATGGCCAACGCAGACGCCAGGAACATGGGCGCCGACCTGTGCCGCCAGGATC
  AAACATGCACCTACTACTTCAGTGTGGATGCTGACGTGGCCCTGACCGAGCCGAACAGCCTACGGCTCCT
  GATTGAGCAAAACAAGAATGTCATTGCTCCACTCATGACCCGTCACGGGAGGCTCTGGTCCAACTTCTGG
  GGGGCACTGAGTGCGGATGGGTACTACGCCCGCTCGGAGGACTACGTGGACATCGTGCAGGGCCGTCGTG
  TTGGCGTCTGGAATGTGCCTTACATCTCAAACATCTACCTGATCAAGGGCAGTGCCCTGCGGGCCGAGCT
  GCGGCACGTAGACCTGTTCCACTACAGCAAGCTGGATCCTGACATGAGTTTCTGCGCCAATGTCCGACAG
  CAGGAGGTGTTCATGTTCCTGACCAACCGGCATACCTTTGGCCACCTGCTGTCGCTGGATAATTACCAGA
  CCACCCACCTACATAATGATCTCTGGGAGGTGTTCAGCAACCCTGAGGATTGGAAAGAGAAGTACATCCA
  CGAGAATTACACCAAGGCCCTGGCAGGGAAGCTGGTGGAGACGCCTTGTCCGGATGTCTACTGGTTCCCC
  ATCTTCACGGAGGTGGCCTGTGATGAGCTGGTGGAGGAAATGGAACACTACGGCCAATGGTCTCTGGGTG
  ACAATAAGGACAACCGTATCCAGGGTGGCTATGAAAACGTGCCCACTATTGACATCCACATGAACCAGAT
  CACCTTCGAGCGGGAGTGGCACAAGTTCCTGGTGGAGTACATCGCCCCCCTGACAGAGAAGCTGTACCCC
  GGCTACTACACCAAGGCCCAGTTTGATCTAGCCTTCGTCGTCCGCTATAAGCCTGATGAGCAGCCTTCCT
  TGATGCCTCACCATGACGCCTCTACTTTCACCATCAACATAGCCCTGAACAGGGTTGGGGAAGATTATGA
  GGGTGGAGGTTGCCGATTTCTACGCTACAACTGCTCCGTGAGAGCCCCAAGGAAGGGCTGGGCCCTCATG
  CACCCCGGGCGGCTCACACACTACCACGAGGGGCTTCCCACTACCAAGGGTACCCGCTACATTGCCGTGT
  CCTTCGTCGATCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215422 representing NM_053827
  Red=Cloning site Green=Tags(s)
  
  MRSLLLLASLAWLLLAQAKDDAKLEDNLLVLTVATKETEGFRRFKRSAQFFNYKIQSLGLGEDWSAAGGP
  SAAGGGQKVRLLKKALKKYADKEDLVILFVDSYDVVFASGPRELLKKFQQAKSRVVFSAEELIYPDRRLE
  AKYPTVPDGKRFLGSGGFIGYAPSLSKLVAEWEGQDNDSDQLFYTKIFLDPEKREQINISLDHRCRIFQN
  LDGALDEVVLKFEMGHVRARNLAYDTLPVVIHGNGPTKLQVNYLGNYIPRFWTFETGCTVCDEGLRSLKG
  IGDEALPTVLVGVFIEQPTPFLSLFFRRLLHLRYPQKQMRLFIHNQEQHHKLQVEQFLAEHGGEYQSVKL
  VGPEVRMANADARNMGADLCRQDQTCTYYFSVDADVALTEPNSLRLLIEQNKNVIAPLMTRHGRLWSNFW
  GALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRAELRHVDLFHYSKLDPDMSFCANVRQ
  QEVFMFLTNRHTFGHLLSLDNYQTTHLHNDLWEVFSNPEDWKEKYIHENYTKALAGKLVETPCPDVYWFP
  IFTEVACDELVEEMEHYGQWSLGDNKDNRIQGGYENVPTIDIHMNQITFEREWHKFLVEYIAPLTEKLYP
  GYYTKAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGEDYEGGGCRFLRYNCSVRAPRKGWALM
  HPGRLTHYHEGLPTTKGTRYIAVSFVDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053827
• ORF Size: 2184 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053827.1, NM_053827.2, NP_446279.2
• RefSeq Size: 2912
• RefSeq ORF: 2187
• Locus ID: 116552
• MW: 83.6 kDa
• Gene Summary: catalyzes the hydroxylation of lysine residues in procollagen to form hydroxylysine [RGD, Feb 2006]