Cit (NM_001029911) Rat Tagged ORF Clone

CAT#: RR215463

Cit (Myc-DDK-tagged ORF) - Rat citron (rho-interacting, serine/threonine kinase 21) (Cit), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cit
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215463 representing NM_001029911
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAAGTTCAAGTATGGAGTGCGGAACCCGTCCGAGGCCAGTGCCCCCGAGCCCATTGCCAGTCGGG
  CCTCCAGGCTAAATCTCTTCTTCCAGGGGAAACCGCCCCTCATGACTCAACAGCAGATGTCTGCTCTTTC
  CCGGGAAGGGGTGTTAGATGCCCTCTTTGTTCTCTTGGAAGAGTGCAGTCAGCCTGCCCTCATGAAGATA
  AAGCACGTGAGCAGCTTCGTCCGGAAGTATTCTGACACGATAGCCGAGTTACGGGAGCTCCAGCCGTCGG
  TGAGGGACTTCGAAGTGCGAAGTCTTGTGGGCTGCGGTCACTTCGCCGAAGTGCAGGTGGTTAGAGAGAA
  GGCCACCGGGGATGTCTACGCCATGAAGATCATGAAGAAGGCGGCTTTGCGGGCCCAGGAGCAGGTTTCA
  TTTTTCGAGGAGGAGAGGAACATATTATCCCAGAGCACGAGCCCTTGGATCCCCCAATTACAGTACGCCT
  TTCAGGACAAAAATAACCTTTACCTGGTCATGGAGTATCAGCCTGGAGGGGATTTGCTGTCTCTTCTGAA
  TAGATATGAGGACCAGTTAGATGAAAACATGATTCAGTTTTACCTTGCTGAGCTGATCTTGGCTGTCCAC
  AGCGTGCACCAGATGGGATATGTGCATCGAGACATCAAGCCGGAGAACATCCTCATTGACCGAACGGGAC
  ACATCAAGCTGGTGGATTTTGGATCAGCCGCTAAGATGAATTCAAATAAGGTGGATGCCAAACTCCCTAT
  TGGGACCCCAGATTACATGGCTCCGGAAGTGTTGACTGTGATGAACGAGGACCGAAGGGGCACCTACGGC
  CTGGACTGTGACTGGTGGTCTGTCGGAGTGGTTGCCTATGAGATGCTTTACGGGAAGACCCCATTCACGG
  AGGGAACCTCCGCCCGGACCTTCAACAACATCATGAACTTCCAGCGGTTTTTGAAGTTCCCGGATGACCC
  CAAAGTTAGCAGTGAGCTCCTTGATCTGATTCAGAGTTTGCTGTGTGTCCAGAAAGAGAGACTGAAGTTC
  GAGGGTCTCTGTTGCCACCCTTTCTTTGCCAGAACCGACTGGAATAACATCCGGAACTCTCCTCCCCCCT
  TCGTTCCCACCCTCAAGTCTGACGATGACACCTCCAATTTTGATGAACCAGAGAAGAATTCGTGGGTTTC
  ATCCTCTCCGTGCCAGCTGAGCCCTTCGGGTTTCTCGGGCGAAGAGCTGCCCTTTGTGGGGTTTTCATAC
  AGCAAGGCACTGGGGTATCTTGGTAGATCTGAGTCTGTTGTGTCGGGTCTGGACTCCCCTGCCAAGATTA
  GCTCCATGGAAAAGAAACTTCTCATCAAAAGCAAAGAGCTGCAAGACTCTCAGGACAAGTGTCACAAGAT
  GGAGCAGGAAATGGCCCGGTTGCATCGCAGAGTGTCAGAGGTGGAGGCTGTGCTTAGTCAGAAGGAGGTG
  GAGCTGAAGGCCTCTGAGACTCAGAGATCCCTCCTGGAGCAGGACCTTGCCACCTACATCACAGAATGCA
  GTAGCTTAAAGCGAAGTCTGGAGCAAGCGCGGATGGAGGTGTCTCAGGAGGATGACAAAGCTCTTCAGCT
  TCTCCACGACATCAGAGAGCAGAGCCGGAAGCTCCAGGAGATCAAGGAGCAGGAGTACCAGGCTCAGGTG
  GAGGAGATGAGGCTGATGATGAATCAGCTGGAAGAGGACCTCGTGTCAGCCCGAAGACGCAGCGATCTCT
  ACGAGTCTGAGCTGAGGGAGTCTCGGCTCGCCGCCGAGGAATTCAAGCGGAAGGCAAACGAATGTCAGCA
  TAAACTGATGAAGGCTAAGGACCTAGGGAAGCCTGAAGTGGGAGAATGTTCCAGACTGGAGAAGATCAAT
  GCTGAGCAGCAGCTGAAGATTCAGGAGCTCCAGGAGAAGCTGGAGAAGGCTGTAAAAGCCAGCACCGAGG
  CCACAGAGCTCCTGCAGAACATCCGGCAGGCAAAGGAGCGAGCTGAGAGGGAGCTGGAGAAGCTGCACAA
  CAGGGAAGATTCTTCCGAAGGCATCAAAAAGAAACTGGTGGAAGCTGAGGAACGCCGCCACTCCCTGGAG
  AACAAGGTAAAGAGACTAGAGACCATGGAGCGTAGAGAGAACAGACTGAAGGATGACATCCAGACAAAGT
  CCGAACAGATCCAGCAGATGGCTGATAAAATTCTGGAGCTGGAGGAGAAGCATCGGGAGGCTCAGGTCTC
  AGCTCAACACCTAGAAGTACACCTGAAGCAGAAGGAACAGCACTATGAGGAAAAGATCAAAGTGTTGGAC
  AATCAGATAAAGAAGGACTTGGCCGACAAGGAGAGCCTAGAGACCATGATGCAGAGGCACGAAGAGGAGG
  CCCACGAGAAGGGCAAGATCCTCAGCGAGCAGAAGGCGATGATCAACGCAATGGATTCCAAGATCAGATC
  CCTGGAGCAGAGGATTGTGGAGCTGTCAGAAGCCAACAAGCTTGCGGCAAACAGCAGTCTCTTCACCCAG
  AGGAACATGAAGGCCCAGGAAGAGATGATCTCAGAACTCAGGCAGCAGAAATTTTACCTGGAGACACAGG
  CCGGGAAGCTGGAGGCCCAGAACCGAAAACTGGAAGAGCAACTAGAGAAGATCAGCCACCAAGATCACAG
  TGACAAGAATCGGCTGCTGGAGCTGGAGACAAGGTTGAGAGAGGTCAGCCTGGAGCATGAGGAGCAGAAG
  CTGGAGCTGAAGCGGCAGCTCACGGAGCTGCAGCTGTCCCTGCAGGAGCGTGAGTCCCAGCTGACGGCCC
  TGCAGGCCGCCCGGGCTGCCCTTGAGAGCCAGCTCCGCCAGGCGAAGACGGAGCTGGAGGAGACAACTGC
  AGAAGCGGAGGAGGAGATCCAGGCACTCACGGCACATCGAGATGAAATCCAGCGCAAATTCGATGCCCTT
  CGCAACAGCTGTACTGTCATCACCGACCTGGAGGAGCAGCTGAACCAGCTCACCGAGGACAACGCCGAGC
  TCAACAACCAAAACTTCTACCTGTCCAAACAACTCGATGAGGCTTCGGGTGCCAACGATGAGATTGTGCA
  GCTGCGAAGCGAGGTGGACCATCTCCGCCGTGAGATCACGGAGCGGGAGATGCAGCTCACCAGCCAGAAG
  CAAACAATGGAGGCTCTGAAGACGACCTGCACCATGCTGGAGGAGCAGGTCATGGACCTGGAGGCCCTGA
  ACGACGAGCTGCTGGAGAAGGAGCGCCAGTGGGAGGCCTGGAGGAGCGTCCTCGGGGACGAGAAGTCTCA
  GTTTGAGTGTCGAGTTCGAGAGCTACAGAGGATGCTGGACACCGAGAAGCAGAGCAGGGCTAGAGCTGAT
  CAGCGGATCACGGAGTCTCGCCAGGTGGTGGAGCTGGCAGTGAAGGAGCACAAAGCCGAGATTCTCGCGC
  TGCAGCAGGCTCTCAAAGAGCAGAAGCTGAAAGCTGAGAGCCTGTCGGACAAGCTCAACGACCTGGAGAA
  GAAGCACGCCATGCTTGAGATGAATGCCCGGAGCTTACAGCAGAAACTGGAGACAGAGCGGGAGCTCAAA
  CAGAGGCTTCTGGAGGAGCAAGCCAAGTTGCAGCAGCAGATGGACCTGCAGAAGAACCACATCTTCAGAC
  TGACACAAGGGCTGCAGGAGGCGCTGGACCGGGCGGATCTGCTGAAGACCGAAAGGAGCGACCTGGAATA
  TCAGCTGGAAAACATTCAGGTTCTCTACTCACACGAGAAGGTGAAAATGGAAGGCACTATTTCTCAACAA
  ACCAAACTCATTGATTTCCTGCAAGCCAAAATGGACCAGCCCGCTAAAAAGAAAAAGGTTCCTCTGCAGT
  ACAATGAGCTGAAGCTCGCCCTGGAAAAGGAGAAAGCGCGCTGTGCGGAGCTGGAGGAAGCCCTTCAGAA
  GACCCGCATCGAACTCCGGTCCGCACGGGAGGAAGCTGCTCACCGCAAAGCCACGGACCACCCGCACCCA
  TCTACGCCAGCCACTGCGAGGCAGCAGATCGCCATGTCCGCCATCGTGCGGTCGCCTGAGCACCAGCCCA
  GTGCCATGAGCCTGCTCGCCCCACCATCCAGCCGCAGAAAGGAGGCGTCAACTCCAGAGGAGTTCAGCCG
  GCGTCTTAAAGAACGTATGCACCACAACATTCCTCACCGGTTTAACGTGGGCCTGAATATGCGAGCCACC
  AAGTGCGCCGTGTGTCTGGATACTGTGCACTTTGGACGCCAGGCATCCAAATGTCTCGAATGTCAGGTGA
  TGTGCCATCCCAAGTGTTCCACCTGCTTGCCCGCCACCTGTGGCCTACCTGCCGAATATGCCACACACTT
  CACCGAGGCCTTCTGCCGAGACAAAGTGAGCTCCCCGGGGCTCCAGAGCAAGGAGCCCAGCAGCAGCTTG
  CACCTGGAAGGGTGGATGAAAGTGCCCAGGAATAACAAACGAGGACAGCAAGGCTGGGACAGAAAGTACA
  TTGTCTTAGAGGGTTCAAAAGTCCTCATCTATGACAACGAAGCCAGAGAAGCTGGACAGAGGCCGGTGGA
  AGAATTTGAGCTGTGCCTTCCCGATGGGGATGTATCTATTCATGGTGCCGTTGGTGCTTCTGAACTTGCA
  AATACAGCCAAAGCAGATGTCCCATACATACTGAAGATGGAGTCCCATCCGCATACCACCTGCTGGCCTG
  GGAGAACCCTCTACCTGCTGGCACCCAGCTTTCCTGACAAGCAGCGCTGGGTCACAGCCCTAGAGTCTGT
  CGTCGCAGGTGGGAGAGTTTCTAGGGAAAAGGCAGAGGCCGATGCTAAATTACTTGGAAACTCTCTGCTG
  AAGCTGGAAGGTGACGACCGGCTTGACATGAACTGTACCCTGCCCTTCAGCGACCAGGTGGTGCTGGTGG
  GCACCGAGGAGGGCCTCTATGCGCTGAACGTCTTGAAAAACTCCTTAACCCACATCCCAGGAATCGGAGC
  AGTCTTCCAGATTTACATCATCAAGGACCTGGAGAAGCTGCTCATGATAGCAGGGGAAGAGCGGGCTCTG
  TGTTTGGTGGACGTGAAGAAGGTGAAGCAGTCGTTGGCACAGTCACACCTTCCTGCCCAGCCTGACGTCT
  CCCCCAACATATTTGAAGCCGTCAAAGGCTGCCACCTGTTTGCTGCTGGCAAGATCGAGAACAGCCTCTG
  CATCTGTGCCGCCATGCCGAGCAAAGTCGTCATCCTGCGCTACAACGACAACCTCAGCAAGTTCTGCATC
  CGCAAGGAGATTGAGACCTCTGAGCCCTGCAGCTGTATCCACTTCACCAATTACAGTATCCTCATCGGAA
  CCAACAAGTTCTATGAGATCGACATGAAACAGTACACGCTTGAGGAGTTCCTGGACAAGAATGACCATTC
  CTTGGCACCTGCTGTGTTCGCATCCTCGACCAACAGCTTCCCCGTCTCCATCGTGCAGGCAAACAGCACC
  GGGCAGCGGGAAGAGTACCTGCTGTGTTTCCACGAATTTGGGGTGTTCGTGGATTCTTATGGAAGACGTA
  GCCGCACAGACGATCTCAAGTGGAGTCGCTTACCTCTGGCCTTTGCCTACAGAGAGCCTTATCTGTTTGT
  GACTCACTTTAACTCCCTGGAAGTAATTGAGATCCAGGCACGTTCCTCACTGGGGACCCCTGCCCGAGCC
  TATCTGGAAATCCCAAACCCTCGCTACCTGGGCCCCGCAATTTCCTCCGGAGCAATTTACCTGGCCTCCT
  CATACCAGGACAAGTTAAGGGTCATATGCTGCAAAGGAAACCTCGTGAAGGAGTCTGGCACTGAGCAGCA
  CCGCGTGCCCTCCACATCCCGCAGCAGCCCTAACAAGCGAGGCCCACCAACATACAACGAGCACATCACC
  AAACGTGTGGCCTCCAGCCCAGCACCACCAGAAGGCCCCAGCCATCCCCGAGAGCCAAGCACACCCCACC
  GCTACCGAGACAGAGAGGGCCGGACAGAGCTGCGCAGGGACAAGTCTCCAGGCCGTCCTCTGGAGCGGGA
  GAAGTCGCCAGGCCGGATGCTCAGCACGAGGAGAGAGCGGTCCCCGGGGAGACTGTTTGAAGACAGCAGC
  AGGGGCCGGCTGCCTGCGGGAGCCGTGAGGACCCCACTGTCCCAGGTTAACAAGGTCTGGGACCAGTCTT
  CAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215463 representing NM_001029911
  Red=Cloning site Green=Tags(s)
  
  MLKFKYGVRNPSEASAPEPIASRASRLNLFFQGKPPLMTQQQMSALSREGVLDALFVLLEECSQPALMKI
  KHVSSFVRKYSDTIAELRELQPSVRDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKAALRAQEQVS
  FFEEERNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVH
  SVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYG
  LDCDWWSVGVVAYEMLYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCVQKERLKF
  EGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLSPSGFSGEELPFVGFSY
  SKALGYLGRSESVVSGLDSPAKISSMEKKLLIKSKELQDSQDKCHKMEQEMARLHRRVSEVEAVLSQKEV
  ELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQV
  EEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDLGKPEVGECSRLEKIN
  AEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLE
  NKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLD
  NQIKKDLADKESLETMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQ
  RNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQK
  LELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDAL
  RNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQK
  QTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARAD
  QRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELK
  QRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQ
  TKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHP
  STPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKEASTPEEFSRRLKERMHHNIPHRFNVGLNMRAT
  KCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKVSSPGLQSKEPSSSL
  HLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELA
  NTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLL
  KLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERAL
  CLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKFCI
  RKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSTNSFPVSIVQANST
  GQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARA
  YLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHIT
  KRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSS
  RGRLPAGAVRTPLSQVNKVWDQSSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029911
• ORF Size: 6165 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029911.1, NP_001025082.1
• RefSeq Size: 6291 bp
• RefSeq ORF: 6168 bp
• Locus ID: 83620
• Cytogenetics: 12q16
• MW: 235.3 kDa
• Gene Summary: a serine/threonine kinase; involved in regulating G2/M transition [RGD, Feb 2006]