Micb (NM_001017468) Rat Tagged ORF Clone

CAT#: RR215466

Micb (Myc-DDK-tagged ORF) - Rat Mill2 gene for MHC class I-like located near the LRC, 2, exon 3, partial cds, strain:LEW.1lm1 (Mill2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Micb
• Synonyms: Mill2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215466 representing NM_001017468
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCCAGTTCTGGAACAGCAGGACCAGCAGTCCTACTGTTGATCTTAGCGCTGCTCCTGACAGAGT
  CACAGGGATCCAGGAGCCAGGGAACCCACACTCTGCGCTATGATGTCACAGCCTGTTTTCTGGAGGGCTC
  TGAGCAGACCAGGCTCCTGGTCCTAATATACGTAGACGATGAGCTCTTCCTGAGGTACAATGGAGACAGG
  AGGAGTGCAAAGCCCTGGGCATGCTGGATTAAGGGCCATGGGGGAGATGAGACGTGTGCAAGAGAGGTTG
  AGAATCTGCCGAAGGAGGAGGAGAGACTCAGGGAGATGATGGCTGAGATGATAAACCAGAAGGGCCACGA
  CAAAGGCCCATACACTCTTCAGGCAACCCTGGGCTGTGAGCTTCAAAGAAACGGAAGCACTAGAGGCTTC
  TGGCACCTGGGCTACGAAGGGCGGAACCTCCTCACCTTCGACCAGAAGACTCTCACATGGACAATGGATG
  TGCCCTTCACCCAGCAGAAGAAGACGTTCTGGGAGCCACGTGCACCCAAAGCTGACCTAGTTAAGACGTT
  CTTGGATGAGACATGTCCTGCTCGGCTCCAGAGACACCTGGCTTCCTTGAGTAATGTTCTGCCGGATACA
  GGTTCCCCAGTGGTGATTGTGACCTGCAGGAATTATCCCGTGGGCAGGATCACGCTGACATGCCGGGCTT
  TTAATCTGAGTTCCCATGTGGCCACCCTGCTCTGGCTTCGGGACGGGAAGCCGGTACAGCAGGATGTCTT
  TGGGCCTGGGACCATCCTGCCCAGTGGGGACGGGACCTACCAGACCTGGGTGTCCATTCGGGTTCTTCCT
  GGACAGGAACCACAGTTCGCCTGCAACCTGAGGCATAGCAACCGCACCATCATGCAGACCGCTGTCTCTG
  GCGAGAGCATGGGATGGCCATCCGCCTCATGGGCTACGAGGCAAGAGGCGGAAGGACCCCATAGGACACA
  CAATGACCACGTGGTTGATGGCGGACTGGTTACGGGGAATGCTAATAAGGACAGTCCAGATGCTTCCAGC
  TGTGCTACAGCCTCAGCCATATCTGCGTTCCCCGTTGTCGTGTTGTCTGTGGCACTGCCCAGAGCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215466 representing NM_001017468
  Red=Cloning site Green=Tags(s)
  
  MEASSGTAGPAVLLLILALLLTESQGSRSQGTHTLRYDVTACFLEGSEQTRLLVLIYVDDELFLRYNGDR
  RSAKPWACWIKGHGGDETCAREVENLPKEEERLREMMAEMINQKGHDKGPYTLQATLGCELQRNGSTRGF
  WHLGYEGRNLLTFDQKTLTWTMDVPFTQQKKTFWEPRAPKADLVKTFLDETCPARLQRHLASLSNVLPDT
  GSPVVIVTCRNYPVGRITLTCRAFNLSSHVATLLWLRDGKPVQQDVFGPGTILPSGDGTYQTWVSIRVLP
  GQEPQFACNLRHSNRTIMQTAVSGESMGWPSASWATRQEAEGPHRTHNDHVVDGGLVTGNANKDSPDASS
  CATASAISAFPVVVLSVALPRAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017468
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017468.1, NM_001017468.2, NP_001017468.1
• RefSeq Size: 1202 bp
• RefSeq ORF: 1122 bp
• Locus ID: 365212
• Cytogenetics: 1q21
• MW: 41.1 kDa