Nefm (NM_017029) Rat Tagged ORF Clone

CAT#: RR215467

Nefm (Myc-DDK-tagged ORF) - Rat neurofilament, medium polypeptide (Nefm), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nefm
• Synonyms: Nef3; Nfm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215467 representing NM_017029
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGCGTCACCGAGACCCCGTCCAGCT
  TCAGTCGTGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCAC
  CGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGCCTCGCCTACAGCTCGGCTATGCTCAGCTCG
  GCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCTTCGCTGCTTAACGGCGGCTCCGGCGGCGACTACAAGC
  TGTCCCGCTCAAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGTTTCGCCGGCTACATCGAGAAAGT
  GCACTACTTGGAACAACAGAACAAGGAGATCGAGGCAGAGATCCACGCGCTGCGGCAGAAGCAGGCCTCG
  CACGCCCAGCTGGGTGACGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTGGAGATGGTGAATC
  ACGAGAAGGCTCAAGTGCAGCTGGACTCTGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTT
  CGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATCCGGGCGCTGCGCAAAGACATAGAGGAG
  TCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCCTTCCTGCGGA
  GCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCCCAGATCCAGGCGTCGCACATCACCGTAGAGCGCAA
  AGACTACCTGAAGACAGACATCTCCACGGCGCTGAAAGAGATCCGCTCCCAGCTCGAGTGTCACTCCGAC
  CAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGA
  ACAAGGAGGCCATCCGCTCCGCTAAAGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCAT
  TGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAACGGCAGCTCAGCGACATCGAGGAGCGCCAC
  AACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGCTGGAAAATGAGCTTCGGGGAACAAAGTGGG
  AAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCTCTGGACATCGAGATCGC
  CGCATATAGGAAACTACTGGAGGGTGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACTGGGCCT
  CTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCA
  AGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATTGAGGAGACTAAAGTGGAAGATGAGAAGTCAGA
  AATGGAAGACGCCCTCACAGTCATTGCAGAGGAATTGGCAGCCTCTGCCAAAGAGGAGAAAGAAGAGGCA
  GAAGAAAAGGAAGAGGAACCGGAAGTTGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGG
  AAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAAGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTCAGA
  CCAGGCAGAAGAGGGAGGATCTGAGAAGGAAGGCTCGAGTGAAAAGGATGAAGGTGAGCAAGAAGAAGAA
  GGGGAAACTGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAACAGAGGGAAAGG
  TCGAGGAAATGGCTATCAAGGAGGAAATCAAGGTCGAGAAGCCCGAGAAAGCCAAGTCCCCTGTGCCAAA
  ATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAAGCCAAAGCCGGAAAGGATGAGCAGAAGGAGGAAGAG
  AAAGTTGAGGAGAAGAAGGAGGTAGCCAAGGAATCACCCAAGGAAGAGAAGGTGGAGAAAAAGGAGGAGA
  AGCCAAAAGATGTCCCAGATAAAAAGAAGGCTGAGTCCCCAGTGAAAGAAAAGGCCGTAGAGGAAATGAT
  CACCATTACTAAGTCGGTAAAGGTGAGCCTGGAGAAAGACACCAAAGAGGAGAAGCCTCAGCAGCAGGAG
  AAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGTAGTGAGGAGGAAGTGGGTGACAAAAGCCCGCAAGAAT
  CCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGAAACTCAGGA
  GAAGGGCAGTGGGCAAGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCTGCGGAGGAA
  AAGAAAGGGGAGGATAGAAGTGATGACAAAGTGGTGGTGACCAAGAAGGTAGAAAAAATCACCAGCGAGG
  GAGGCGATGGTGCTACCAAATACATCACCAAATCTGTTACTGTCACTCAAAAGGTTGAAGAGCATGAGGA
  GACCTTTGAGGAGAAGCTGGTGTCAACTAAAAAGGTAGAAAAGGTCACTTCACATGCCATAGTCAAGGAA
  GTCACCCAGGGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215467 representing NM_017029
  Red=Cloning site Green=Tags(s)
  
  MSYTLDSLGNPSAYRRVTETPSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLAYSSAMLSS
  AESSLDFSQSSSLLNGGSGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIHALRQKQAS
  HAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEE
  SSMVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLECHSD
  QNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERH
  NHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFSGSITGP
  LYTHRQPSVTISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEDALTVIAEELAASAKEEKEEA
  EEKEEEPEVEKSPVKSPEAKEEEEGEKEEEEEGQEEEEEEDEGVKSDQAEEGGSEKEGSSEKDEGEQEEE
  GETEAEGEGEEAEAKEEKKTEGKVEEMAIKEEIKVEKPEKAKSPVPKSPVEEVKPKPEAKAGKDEQKEEE
  KVEEKKEVAKESPKEEKVEKKEEKPKDVPDKKKAESPVKEKAVEEMITITKSVKVSLEKDTKEEKPQQQE
  KVKEKAEEEGGSEEEVGDKSPQESKKEDIAINGEVEGKEEEEQETQEKGSGQEEEKGVVTNGLDVSPAEE
  KKGEDRSDDKVVVTKKVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKE
  VTQGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017029
• ORF Size: 2535 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017029.1, NM_017029.2, NP_058725.1
• RefSeq Size: 3067
• RefSeq ORF: 2538
• Locus ID: 24588
• MW: 95.6 kDa
• Gene Summary: neuronal intermediate filament protein; important component of the cytoskeleton [RGD, Feb 2006]