Sms (NM_001033899) Rat Tagged ORF Clone

CAT#: RR215558

Sms (Myc-DDK-tagged ORF) - Rat spermine synthase (Sms), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sms
• Synonyms: MGC124869
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215558 representing NM_001033899
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGCAAGGCACAGCACGCTCGACTTCACGCTCGGCGCCAAAGCTGATGGTGAGGCCATTCTGA
  AAGGCCTTCAGTCCATCTTTCAGGAGCAGGGGATGGCAGAGTCGGTGCACACCTGGCAGGACCATGGGTA
  TTTAGCAACCTACACGAACAAGAATGGCAGCTTTGCCAATTTGAGAATTTATCCACATGGATTGGTGTTG
  CTGGACCTTCAGAGTTACGACAGTGATGTGCAAGGCAAACAAGAAACTGACTGCCTTTTGAATAAAATAG
  AAGAAAAAATGAAAGAATTGAGTCAAGACAGTACTGGTCGGGTGAAGCGATTACCACCCATAGTTCGAGG
  AGGGGCCATTGACAGATACTGGCCTACTGCTGACGGGCGCCTGGTTGAGTATGACATAGATGAAGTGGTG
  TATGACGAAGACTCACCTTATCAGAACATTAAAATTCTTCACTCAAAGCAGTTTGGGAATATTCTCATCC
  TCAGCGGGGATGTTAATTTGGCAGAGAGTGACTTGGCATATACCAGGGCCATCATGGGCAATGGTAAAGA
  AGACTACACTGGCAAAGATGTACTGATTCTGGGAGGCGGCGATGGAGGCATATTATGTGAAATTGTCAAA
  CTGAAACCAAAGATGGTCACTATGGTAGAGATTGACCAGATGGTAATTGATGGATGTAAGAAATACATGC
  GCAGAACATGTGGAGATGTCTTAGACAATCTTAAAGGAGATTGCTATCAGGTTCTTATAGAAGACTGTAT
  TCCAGTACTGAAGAGGTACGCCAAAGAAGGGAGAGAGTTTGATTATGTGATTAATGATTTGACAGCTGTC
  CCTATCTCTACATCTCCAGAAGAAGATTCCACATGGGAGTTTCTCAGACTGATTCTTGACCTGTCAATGA
  AGGTTCTGAAACAGGATGGAAAATATTTCACACAGGGGAACTGTGTCAACTTGACTGAAGCCCTGTCGCT
  CTATGAAGAACAGCTGGGGCGCCTGTATTGTCCTGTGGAATTCTCAAAAGAGATTGTCTGTGTCCCTTCA
  TACTTGGAATTGTGGGTATTTTACACTGTTTGGAAGAAAGCTAAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215558 representing NM_001033899
  Red=Cloning site Green=Tags(s)
  
  MAAARHSTLDFTLGAKADGEAILKGLQSIFQEQGMAESVHTWQDHGYLATYTNKNGSFANLRIYPHGLVL
  LDLQSYDSDVQGKQETDCLLNKIEEKMKELSQDSTGRVKRLPPIVRGGAIDRYWPTADGRLVEYDIDEVV
  YDEDSPYQNIKILHSKQFGNILILSGDVNLAESDLAYTRAIMGNGKEDYTGKDVLILGGGDGGILCEIVK
  LKPKMVTMVEIDQMVIDGCKKYMRRTCGDVLDNLKGDCYQVLIEDCIPVLKRYAKEGREFDYVINDLTAV
  PISTSPEEDSTWEFLRLILDLSMKVLKQDGKYFTQGNCVNLTEALSLYEEQLGRLYCPVEFSKEIVCVPS
  YLELWVFYTVWKKAKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033899
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033899.1, NP_001029071.1
• RefSeq Size: 1675 bp
• RefSeq ORF: 1101 bp
• Locus ID: 363469
• Cytogenetics: Xq21
• MW: 41.3 kDa