Nampt (NM_177928) Rat Tagged ORF Clone

CAT#: RR215629

Nampt (Myc-DDK-tagged ORF) - Rat nicotinamide phosphoribosyltransferase (Nampt), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nampt
• Synonyms: Pbef; Pbef1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215629 representing NM_177928
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCTGCGGCAGAAGCCGAGTTCAACATCCTGCTGGCCACCGACTCGTACAAGGTTACTCACTATA
  AACAATACCCACCCAACACAAGCAAAGTTTATTCCTACTTTGAATGCCGTGAAAAGAAGACAGAAAACTC
  CAAAGTAAGGAAGGTGAAATACGAGGAAACAGTATTTTATGGGTTGCAGTACATTCTTAATAAGTACTTA
  AAAGGTAAAGTAGTGACCAAAGAGAAAATCCAGGAGGCCAAAGAAGTGTACAGAGAACATTTCCAAGATG
  ATGTCTTCAATGAAAGGGGATGGAACTACATCCTTGAGAAATATGACGGTCATCTCCCAATTGAAGTAAA
  GGCTGTTCCTGAGGGCTCTGTCATCCCCAGAGGGAACGTGCTGTTCACAGTGGAAAACACAGACCCAGAG
  TGCTACTGGCTCACCAACTGGATTGAGACTATTCTTGTCCAGTCCTGGTATCCAATTACAGTGGCCACAA
  ATTCAAGAGAGCAGAAGAAAATCCTGGCCAAATATTTGTTAGAAACTTCTGGTAACTTAGACGGTCTGGA
  ATATAAGTTACATGACTTCGGCTACAGAGGAGTCTCTTCACAAGAGACTGCCGGCATAGGGGCATCTGCT
  CATTTGGTTAACTTCAAAGGAACAGATACTGTGGCGGGAATTGCTCTAATTAAAAAATACTATGGGACAA
  AAGATCCTGTTCCAGGCTATTCTGTTCCAGCGGCAGAGCACAGTACCATAACAGCGTGGGGGAAAGACCA
  TGAAAAAGATGCTTTTGAACACATAGTAACACAGTTCTCATCAGTGCCTGTGTCTGTGGTCAGCGATAGC
  TATGACATCTATAACGCGTGTGAGAAAATATGGGGTGAAGACCTGAGACACCTGATAGTGTCGAGAAGTA
  CAGAGGCACCACTGATCATCAGACCTGATTCTGGAAATCCGCTCGACACTGTCCTGAAGGTCTTAGATAT
  ATTAGGCAAGAAGTTCCCTGTCTCTGAGAACTCCAAAGGCTACAAGTTGCTGCCACCTTACCTTAGAGTC
  ATTCAAGGGGACGGAGTGGATATCAATACTCTACAGGAGATTGTCGAGGGAATGAAACAGAAAAAGTGGA
  GTATTGAGAATGTCTCCTTTGGTTCTGGTGGCGCTTTGCTACAGAAGTTAACCAGAGACCTCTTGAATTG
  CTCCTTCAAGTGCAGCTATGTTGTAACCAATGGCCTTGGGGTTAATGTCTTTAAGGACCCAGTTGCTGAT
  CCCAACAAAAGGTCGAAAAAGGGCCGGCTGTCTTTACATAGGACACCAGCAGGGACCTTTGTCACCCTTG
  AAGAAGGAAAAGGAGACCTTGAAGAATATGGCCATGATCTTCTCCATACGGTTTTCAAGAATGGGAAGGT
  GACAAAAAGCTACTCGTTTGACGAAGTCAGAAAAAATGCACAGCTGAACATGGAGCAGGATGTGGCGCCT
  CAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215629 representing NM_177928
  Red=Cloning site Green=Tags(s)
  
  MNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYL
  KGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPE
  CYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASA
  HLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDS
  YDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVSENSKGYKLLPPYLRV
  IQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVAD
  PNKRSKKGRLSLHRTPAGTFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNMEQDVAP
  H
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177928
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177928.1, NM_177928.2, NM_177928.3, NP_808789.1
• RefSeq Size: 2590
• RefSeq ORF: 1476
• Locus ID: 297508
• MW: 55.4 kDa
• Gene Summary: has similarity to cytokines but is not secreted; localized to the nucleus and cytoplasm, changes in subcellular localization may be associated with the cell cycle [RGD, Feb 2006]