Casp8 (NM_022277) Rat Tagged ORF Clone

CAT#: RR215647

Casp8 (Myc-DDK-tagged ORF) - Rat caspase 8 (Casp8), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Casp8
• Synonyms: CASP-8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215647 representing NM_022277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCCACAGTTGTCTTTACGATATTGCTGAACGTCTGGGCAACGAAGAACTGGCTGCCCTCAAGT
  TCCTGTGCTTGGACCACATCCCGCAGAAGAAGCAGGAGTCCATCAATGATGTCCTGGTGCTATTTCAGAG
  GCTGCAGGAAGAGGGGATGTTGGAGGAAGACAATTTGTCCTTCCTGAAGGAGCTGCTTTTCCATATCAGT
  CGGCGGGATCTGCTGAGCAGAGTCCTAAAAAGCAGCCCAGAGGAAATGGTGAGGGAGCTGCAGGTTCTGG
  GCAAAGCCCAGGTTTCTGCCTACAGGGTTATGCTCTTTAAGCTCTCAGAAGACATGGACAAGGAGGATTT
  GAAATCTTTTAAGTTCCTTTTGATCACTGAGATCCCTAAATGTAAACTGCAAGACAACTCGAGCCTGCTT
  GATATTTTCGTAGAGATGGAGAAGAGGACTATCCTGGCAGAAAACAACTTGGTTACCCTAAAATCAATCT
  GTTTCCGGGTCAACAGGAGCTTGCTGGGAAGGATCGACGATTACGAACGATCAAGCACAGAGAGAAGAAT
  GAGCACTGAAGGAGGGGAAGAGCTGCCAGTTTCTGTTTTGGATGAGGTGACCATCAAAATGCAGGACATG
  TGGGACTCGCCAGGAGAGCAAGAGAGTGAGTCACTAAATTCAGACAACGTTTACCAAATGAAGAGCAAAC
  CTCGGGGGTACTGTCTGATCTTCAACAATAATAATTTCAGCAAAGCACGGGAGGACATACCCAAACTCAG
  CAACATGCGGGACAGAAAAGGAACCAACTATGATGAAGAGGCTCTGAGTAAGACCTTTAAGGAGCTTCAT
  TTTGAGATCGTGTCCTTCAGTGATTGCACAGCAAGTCAAATCCATGAGGTTCTAGTATCCTATCAAAGCA
  AGGACCACAAGGGCAAAGACTGCTTCATCTGCTGCATCCTATCACATGGGGACAAGGGCATCGTCTACGG
  AACGGATGGGAAGGAAGCCTCTATCTATGAGCTGACATCTTACTTCACTGGCTCAAAGTGCCCTTCCCTG
  GCTGGAAAACCCAAAATCTTTTTTATTCAAGCTTGTCAAGGAAATAACTTCCAGAAAGCGGTGCCTGTGC
  CTGATGAGACAGGCTTAGAACAGGAGCACGTTTTAGAAGAGGATTCATCATCTTACAAGAACTATATCCC
  AGATGAGGCAGACTTTCTGCTGGGGATGGCTACTGTGAAAAACTGTGTTTCCTACCGAGATCCCACGCGT
  GGGACCTGGTATATCCAGTCACTTTGCCAGAGCCTGAGGGAAAGATGTCCTCGAGGTGAGGATATTCTCA
  GCATCCTGACTGGCGTGAACTATGATGTGAGCAATAAAGACAACCCGAGGAACATGGGAAAGCAGATGCC
  ACAGCCCATCTTCACACTACGGAAGAAGCTCTTCTTCCCTCCTAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215647 representing NM_022277
  Red=Cloning site Green=Tags(s)
  
  MDFHSCLYDIAERLGNEELAALKFLCLDHIPQKKQESINDVLVLFQRLQEEGMLEEDNLSFLKELLFHIS
  RRDLLSRVLKSSPEEMVRELQVLGKAQVSAYRVMLFKLSEDMDKEDLKSFKFLLITEIPKCKLQDNSSLL
  DIFVEMEKRTILAENNLVTLKSICFRVNRSLLGRIDDYERSSTERRMSTEGGEELPVSVLDEVTIKMQDM
  WDSPGEQESESLNSDNVYQMKSKPRGYCLIFNNNNFSKAREDIPKLSNMRDRKGTNYDEEALSKTFKELH
  FEIVSFSDCTASQIHEVLVSYQSKDHKGKDCFICCILSHGDKGIVYGTDGKEASIYELTSYFTGSKCPSL
  AGKPKIFFIQACQGNNFQKAVPVPDETGLEQEHVLEEDSSSYKNYIPDEADFLLGMATVKNCVSYRDPTR
  GTWYIQSLCQSLRERCPRGEDILSILTGVNYDVSNKDNPRNMGKQMPQPIFTLRKKLFFPPN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022277
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022277.1, NP_071613.1
• RefSeq Size: 2003
• RefSeq ORF: 1449
• Locus ID: 64044
• MW: 55.3 kDa
• Gene Summary: member of the cysteine-aspartic acid protease (caspase) familythat mediates the terminal stage of apoptosis; involved in apoptosis induced by Fas and other stimuli [RGD, Feb 2006]