Mapk9 (NM_001270545) Rat Tagged ORF Clone

CAT#: RR216529

Mapk9 (myc-DDK-tagged) - Rat mitogen-activated protein kinase 9 (Mapk9), transcript variant 3

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapk9
• Synonyms: SAPK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216529 representing NM_001270545
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACAGTAAAAGCGATGGCCAGTTTTACAGTGTGCAAGTGGCAGACTCAACTTTCACTGTTCTAA
  AACGTTACCAGCAGTTGAAACCAATTGGCTCTGGAGCCCAAGGAATTGTTTGTGCTGCTTTTGATACAGT
  TCTTGGAATAAATGTTGCTGTCAAGAAGTTAAGTCGTCCTTTTCAGAACCAAACGCATGCAAAGAGAGCC
  TACCGTGAACTTGTCCTCCTAAAGTGTGTCAATCATAAAAATATAATTAGCTTGTTAAATGTGTTCACAC
  CACAAAAAACGCTAGAAGAATTCCAAGATGTGTACTTGGTTATGGAGTTAATGGACGCTAACTTATGTCA
  GGTTATTCATATGGAGCTGGACCATGAAAGAATGTCATACCTCCTCTACCAGATGCTTTGTGGCATTAAG
  CACCTGCATTCAGCTGGCATAATTCATAGGGATTTGAAGCCTAGCAACATTGTAGTAAAATCAGACTGTA
  CTCTCAAGATCCTTGACTTTGGCCTGGCACGGACAGCCTGTACCAACTTTATGATGACTCCCTATGTGGT
  AACTCGCTACTATCGGGCTCCAGAAGTCATCCTGGGCATGGGCTACAAGGAGAATGTTGATATCTGGTCA
  GTGGGTTGCATCATGGGAGAGCTGGTGAAAGGTTGTGTGATATTCCAAGGTACTGACCATATTGATCAAT
  GGAATAAAGTTATTGAACAGCTAGGAACACCATCCGCAGAGTTCATGAAGAAACTTCAGCCAACTGTAAG
  GAATTATGTGGAAAACAGACCAAAGTACCCTGGAATCAAATTTGAAGAGCTCTTTCCAGATTGGATATTT
  CCGTCAGAATCCGAACGAGACAAAATAAAAACAAGTCAAGCCAGAGATCTGTTATCGAAAATGTTAGTGA
  TTGATCCGGACAAGCGGATCTCTGTGGACGAAGCCTTGCGCCACCCGTATATTACTGTTTGGTATGACCC
  CGCTGAAGCAGAAGCGCCACCACCTCAAATTTATGATGCCCAGTTGGAAGAAAGAGAGCATGCGATTGAA
  GAGTGGAAAGAACTAATTTACAAAGAAGTGATGGACTGGGAAGAAAGAAGCAAGAATGGGGTGAAAGACC
  AGCCTTCAGCACAGATGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216529 representing NM_001270545
  Red=Cloning site Green=Tags(s)
  
  MSDSKSDGQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRA
  YRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIK
  HLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWS
  VGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIF
  PSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIE
  EWKELIYKEVMDWEERSKNGVKDQPSAQMQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270545
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270545.1, NP_001257474.1
• RefSeq Size: 3716
• RefSeq ORF: 1146
• Locus ID: 50658
• MW: 43.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases are multifunctional proteins. They are involved in a wide variety of cellular processes such as growth, proliferation, differentiation, transcription regulation, and development. They function in stress responses, apoptosis, inflammation and transformation. For example, this kinase plays a dominant role in mediating proliferation of lung cancer and prostate cancer cells, and in regulation of osmotic stress-induced tight junction disruption. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2012]