Ddc (NM_001270853) Rat Tagged ORF Clone

CAT#: RR216680

Ddc (myc-DDK-tagged) - Rat dopa decarboxylase (aromatic L-amino acid decarboxylase) (Ddc), transcript variant 2

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddc
• Synonyms: AADC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216680 representing NM_001270853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCCCGTGAATTCCGGAGAAGAGGGAAGGAGATGGTGGATTATATAGCTGACTATCTGGACGGCA
  TTGAGGGACGTCCAGTGTACCCTGACGTGGAGCCTGGCTACCTTCGGGCCCTGATCCCCACCACTGCCCC
  CCAGGAGCCAGAAACATATGAGGACATAATCAGAGACATTGAAAAGATAATCATGCCAGGGGTCACACAC
  TGGCACAGCCCCTACTTCTTCGCTTACTTCCCCACGGCCAGCTCCTACCCAGCTATGCTTGCGGACATGC
  TGTGCGGGGCTATCGGCTGCATTGGCTTCTCCTGGGGAAGTGCCAGCGAAGCCACCTTGGTGGCCCTACT
  GGCTGCTCGGACTAAAATGATCCGCCAGCTGCAGGCAGCCTCCCCAGAGCTGACACAAGCTGCTCTCATG
  GAAAAGCTTGTCGCTTACACATCTGATCAGGCACATTCCTCCGTAGAAAGAGCTGGATTAATTGGTGGAG
  TCAAAATAAAAGCAATTCCTTCAGATGGCAACTACTCCATGAGAGCTGCTGCCCTTCGGGAGGCCCTGGA
  GAGAGACAAGGCGGCTGGCCTGATTCCTTTCTTCGTGGTTGTCACCCTAGGAACCACATCTTGCTGCTCT
  TTTGACAATCTCCTAGAAGTGGGTCCCATCTGCAACCAGGAGGGTGTATGGCTGCACATTGATGCTGCAT
  ACGCAGGCAGTGCCTTTATCTGTCCTGAGTTCCGGTATCTTCTGAATGGCGTGGAGTTTGCAGATTCCTT
  TAACTTTAATCCCCACAAGTGGCTTTTGGTGAATTTTGACTGCTCTGCCATGTGGGTGAAGAAGAGAACT
  GACCTAACCGAAGCCTTTAATATGGACCCTGTTTATCTGAGGCACAGTCACCAGGACTCAGGACTCATCA
  CTGACTACAGGCACTGGCAAATCCCACTGGGGCGAAGATTTCGCTCCCTGAAAATGTGGTTTGTTTTTAG
  AATGTACGGAGTCAAGGGGCTGCAGGCTTACATTCGAAAGCACGTGAAGCTGTCTCATGAGTTTGAGTCC
  CTGGTACGCCAGGACCCTCGCTTTGAAATTTGCACGGAAGTCATCCTCGGGTTGGTCTGCTTCCGGCTAA
  AGGGCTCCAACCAGTTGAACGAAACTCTCTTACAAAGAATAAACAGCGCCAAAAAAATCCACTTGGTTCC
  GTGTCGTCTCCGAGACAAGTTTGTGCTGCGCTTTGCGGTGTGCTCCCGCACTGTGGAGTCTGCCCACGTG
  CAGCTGGCCTGGGAGCACATCCGAGATCTAGCGAGCAGTGTGCTGAGGGCAGAGAAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216680 representing NM_001270853
  Red=Cloning site Green=Tags(s)
  
  MDSREFRRRGKEMVDYIADYLDGIEGRPVYPDVEPGYLRALIPTTAPQEPETYEDIIRDIEKIIMPGVTH
  WHSPYFFAYFPTASSYPAMLADMLCGAIGCIGFSWGSASEATLVALLAARTKMIRQLQAASPELTQAALM
  EKLVAYTSDQAHSSVERAGLIGGVKIKAIPSDGNYSMRAAALREALERDKAAGLIPFFVVVTLGTTSCCS
  FDNLLEVGPICNQEGVWLHIDAAYAGSAFICPEFRYLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRT
  DLTEAFNMDPVYLRHSHQDSGLITDYRHWQIPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVKLSHEFES
  LVRQDPRFEICTEVILGLVCFRLKGSNQLNETLLQRINSAKKIHLVPCRLRDKFVLRFAVCSRTVESAHV
  QLAWEHIRDLASSVLRAEKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270853
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270853.1, NP_001257782.1
• RefSeq Size: 4988
• RefSeq ORF: 1323
• Locus ID: 24311
• MW: 49.9 kDa
• Gene Summary: catalyzes the decarboxylation of L-5-hydroxytryptophan to serotonin and L-3,4-dihydroxyphenylalanine to dopamine [RGD, Feb 2006]