Becn1 (NM_001034117) Rat Tagged ORF Clone

CAT#: RR216694

Becn1 (myc-DDK-tagged) - Rat beclin 1, autophagy related (Becn1), transcript variant 2

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Becn1
• Synonyms: Beclin1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216694 representing NM_001034117
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGTCTAAGGCGTCCAGCAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGCTGTAGCCAGCCTC
  TGAAACTGGACACGAGCTTCAAGATCCTGGACCGAGTGACCATTCAGGAACTCACAGCTCCATTACTTAC
  CACAGCCCAGGCGAAACCAGGAGAGAGCCAGGAGGAAGAGGCTAACTCAGGAGAGGAGCCATTTATTGAA
  ACTCGCCAGGATGGTGTCTCTCGAAGATTCATCCCCCCAGCCAGGATGATGTCTACAGAAAGTGCTAATA
  GCTTCACTCTGATCGGGGAGGCATCTGATGGTGGCACCATGGAGAACCTCAGCCGGAGACTCAAGGTCAC
  TGGAGACCTTTTTGACATCATGTCTGGCCAGACAGATGTGGATCACCCACTGTGTGAGGAATGCACAGAT
  ACTCTTTTAGACCAGCTGGACACTCAGCTCAATGTTACTGAAAACGAGTGTCAGAACTACAAACGCTGTT
  TGGAGATGTTGGAGCAAATGAATGAGGGCGACAGTGAACAGCTACAGAGGGAGCTGAAGGAGTTGGCCTT
  GGAGGAGGAGAGGCTGATCCAGGAGCTGGAAGATGTGGAAAAAAACCGAAAGGTGGTGGCAGAAAACCTG
  GAGAAGGTCCAGGCTGAGGCGGAGAGACTGGACCAGGAGGAAGCTCAGTACCAGCGAGAATATAGTGAAT
  TTAAAAGGCAGCAGCTGGAGCTGGATGATGAGCTCAAGAGTGTAGAGAACCAGATGCGCTATGCCCAGAT
  GCAGCTGGACAAGCTCAAGAAAACCAATGTCTTCAATGCGACCTTCCATATCTGGCACAGCGGACAATTT
  GGCACGATCAATAATTTCAGACTGGGTCGCTTGCCCAGTGCTCCTGTGGAATGGAATGAAATCAATGCTG
  CCTGGGGCCAGACAGTGTTGTTGCTCCATGCTTTGGCCAATAAGATGGGTCTGAAATTTCAGAGGTACCG
  ACTTGTTCCCTATGGAAATCACTCGTATCTGGAGTCCCTGACAGACAAATCTAAGGAGTTGCCGTTGTAC
  TGTTCTGGGGGTTTGCGGTTTTTCTGGGACAACAAGTTTGACCATGCAATGGTAGCTTTTCTGGACTGTG
  TGCAGCAGTTCAAAGAAGAGGTGGAAAAAGGAGAGACTCGATTTTGTCTTCCGTACAGGATGGACGTGGA
  GAAAGGCAAGATTGAAGACACTGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAACTCTGAG
  GAGCAGTGGACAAAGGCGCTCAAGTTCATGCTGACGAATCTCAAGTGGGGTCTTGCTTGGGTGTCCTCAC
  AGTTCTATAACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216694 representing NM_001034117
  Red=Cloning site Green=Tags(s)
  
  MEGSKASSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGESQEEEANSGEEPFIE
  TRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTD
  TLLDQLDTQLNVTENECQNYKRCLEMLEQMNEGDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENL
  EKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQMQLDKLKKTNVFNATFHIWHSGQF
  GTINNFRLGRLPSAPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLY
  CSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSE
  EQWTKALKFMLTNLKWGLAWVSSQFYNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034117
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034117.1, NP_001029289.1
• RefSeq Size: 2118 bp
• RefSeq ORF: 1347 bp
• Locus ID: 114558
• Cytogenetics: 10q31
• MW: 51.6 kDa
• Gene Summary: mouse homolog is a haploinsufficient tumor suppressor gene; plays a role in early embryonic development [RGD, Feb 2006]