Slc1a3 (NM_001289942) Rat Tagged ORF Clone

CAT#: RR216794

Slc1a3 (myc-DDK-tagged) - Rat solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), transcript variant Glast1b

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc1a3
• Synonyms: EAAT1; GLAST; GLAST-1; GluT-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216794 representing NM_001289942
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAAAAGCAACGGAGAAGAGCCCAGGATGGGAAGCAGGATGGAAAGATTCCAGCAAGGGGTGCGCA
  AGCGGACGCTCCTGGCCAAGAAGAAAGTTCAGAACATCACCAAGGAGGATGTGAAGAGCTACCTGTTTCG
  GAATGCCTTTGTGCTACTCACCGTCAGCGCTGTCATTGTGGGTACAATCCTTGGATTTGCCCTCCGACCG
  TATAAAATGAGCTACCGGGAGGTCAAGTACTTCTCCTTTCCTGGGGAGCTTCTGATGCGGATGCTGCAGA
  TGTTGGTCTTACCCCTGATCATCTCCAGTCTTGTCACAGGAATGGCGGCCCTAGATAGTAAGGCATCTGG
  GAAGATGGGGATGCGAGCTGTGGTCTATTACATGACTACCACCATCATTGCTGTGGTGATCGGCATAATC
  ATTGTCATCATCATCCACCCCGGAAAGGGCACGAAAGAAAACATGTACAGAGAAGGTAAAATCGTGCAGG
  TGACTGCCGCAGATGCCTTCCTGGATTTAATCAGGAACATGTTCCCACCCAATCTGGTAGAAGCCTGCTT
  TAAACAGTTTAAAACCAGCTATGAGAAGAGAAGCTTTAAAGTGCCCATCCAGGCCAACGAAACACTGTTG
  GGCGCCGTGATCAACAACGTGTCAGAGGCCATGGAGACTCTGACCCGGATCCGGGAGGAGATGGTACCCG
  TTCCTGGGTCTGTGAATGGGGTCAATGCCCTGGGCCTCGTTGTCTTCTCCATGTGCTTCGGCTTCGTGAT
  CGGAAACATGAAGGAGCAGGGGCAAGCGCTAAGAGAGTTCTTCGACTCTCTCAATGAAGCCATCATGAGA
  TTGGTAGCGGTGATAATGTGGTATGCACCTCTGGGCATCCTCTTCTTGATCGCAGGGAAGATTGTTGAGA
  TGGAAGATATGGGTGTGATTGGGGGGCAGCTTGCCATGTATACAGTGACAGTCATCGTCGGCCTCCTCAT
  TCATGCCGTCATCGTCCTGCCTCTCCTCTACTTCCTGGTAACCCGGAAGAACCCCTGGGTTTTCATTGGA
  GGGTTGCTGCAAGCACTCATCACAGCCCTGGGGACCTCCTCAAGTTCTGCCACCCTGCCCATCACTTTCA
  AGTGCCTGGAAGAAAACAATGGTGTGGACAAACGCATCACCAGATTTGTGCTTCCCGTGGGGGCCACCAT
  TAACATGGATGGGACCGCCCTCTACGAGGCTTTGGCCGCCATTTTCATCGCTCAAGTTAACAACTTTGAC
  CTGAATTTTGGACAGATTATAACAATAAGGGACCGCCTCCGAACCACCACCAACGTGCTGGGTGACTCCC
  TCGGGGCCGGGATTGTTGAACACTTGTCCCGACATGAACTGAAGAACCGAGATGTTGAAATGGGGAACTC
  CGTGATTGAGGAGAATGAAATGAAAAAGCCGTATCAGCTGATTGCCCAGGACAATGAACCAGAGAAACCC
  GTGGCAGACAGCGAAACCAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216794 representing NM_001289942
  Red=Cloning site Green=Tags(s)
  
  MTKSNGEEPRMGSRMERFQQGVRKRTLLAKKKVQNITKEDVKSYLFRNAFVLLTVSAVIVGTILGFALRP
  YKMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGII
  IVIIIHPGKGTKENMYREGKIVQVTAADAFLDLIRNMFPPNLVEACFKQFKTSYEKRSFKVPIQANETLL
  GAVINNVSEAMETLTRIREEMVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMR
  LVAVIMWYAPLGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIG
  GLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLPVGATINMDGTALYEALAAIFIAQVNNFD
  LNFGQIITIRDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNEPEKP
  VADSETKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289942
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001289942.1, NP_001276871.1
• RefSeq Size: 3797
• RefSeq ORF: 1497
• Locus ID: 29483
• MW: 55.3 kDa
• Gene Summary: carries out Na+ dependent transport of glutamate and aspartate; may regulate neurotransmitter concentration in the central nervous system [RGD, Feb 2006]