Gck (NM_001270849) Rat Tagged ORF Clone

CAT#: RR216795

Gck (myc-DDK-tagged) - Rat glucokinase (Gck), transcript variant 1

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gck
• Synonyms: GLK; GLUKA; RNGK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216795 representing NM_001270849
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATGGATACTACAAGGTGTGGAGCCCAGTTGTTGACTCTGGGCACCAACAAATGCACGAATGCAT
  GCTCCCTGCTGTGCAGAGCGGGTACTCACAACGGGCACATGAACCCTCGCTGCCGAACAGAGCAGGCGGC
  CGCCACACAGCTGCCCACCTGCAGAGTCCAGCTACTCTTGAACTATCATGTGGAGGGTCGAGCAGATCCT
  GGCAGAGTTCCAGCTGCAGGAGGAAGACCTGAAGAAGGTGATGAGCCGGATGCAGAAGGAGATGGACCGT
  GGCCTGAGGCTGGAGACCCACGAGGAGAAGTCGGAGACTTTCTCTCCTTAGACCTGGGAGGAACCAACTT
  CAGAGTGATGCTGGTCAAAGTGGGAGAGGGGGAGGCAGGGCAGTGGAGCGTGAAGACAAAACACCAGATG
  TACTCCATCCCCGAGGACGCCATGACGGGCACTGCCGAGATGCTCTTTGACTACATCTCTGAATGCATCT
  CTGACTTCCTTGACAAGCATCAGATGAAGCACAAGAAACTGCCCCTGGGCTTCACCTTCTCCTTCCCTGT
  GAGGCACGAAGACCTAGACAAGGGCATCCTCCTCAATTGGACCAAGGGCTTCAAGGCCTCTGGAGCAGAA
  GGGAACAACATCGTAGGACTTCTCCGAGATGCTATCAAGAGGAGAGGGGACTTTGAGATGGATGTGGTGG
  CAATGGTGAACGACACAGTGGCCACAATGATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCAT
  GATTGTGGGCACTGGCTGCAATGCCTGCTACATGGAGGAAATGCAGAATGTGGAGCTGGTGGAAGGGGAT
  GAGGGACGCATGTGCGTCAACACGGAGTGGGGCGCCTTCGGGGACTCGGGCGAGCTGGATGAGTTCCTAC
  TGGAGTATGACCGGATGGTGGATGAAAGCTCAGCGAACCCCGGTCAGCAGCTGTACGAGAAGATCATCGG
  TGGGAAGTATATGGGCGAGCTGGTACGACTTGTGCTGCTTAAGCTGGTGGACGAGAACCTTCTGTTCCAC
  GGAGAGGCCTCGGAGCAGCTGCGCACGCGTGGTGCTTTTGAGACCCGTTTCGTGTCACAAGTGGAGAGCG
  ACTCCGGGGACCGAAAGCAGATCCACAACATCCTAAGCACTCTGGGGCTTCGACCCTCTGTCACCGACTG
  CGACATTGTGCGCCGTGCCTGTGAAAGCGTGTCCACTCGCGCCGCCCATATGTGCTCCGCAGGACTAGCT
  GGGGTCATAAATCGCATGCGCGAAAGCCGCAGTGAGGACGTGATGCGCATCACTGTGGGCGTGGATGGCT
  CCGTGTACAAGCTGCACCCGAGCTTCAAGGAGCGGTTTCACGCCAGTGTGCGCAGGCTGACACCCAACTG
  CGAAATCACCTTCATCGAATCAGAGGAGGGCAGCGGCAGGGGAGCCGCGCTGGTCTCTGCGGTGGCCTGC
  AAGAAGGCTTGCATGCTGGCCCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216795 representing NM_001270849
  Red=Cloning site Green=Tags(s)
  
  MAMDTTRCGAQLLTLGTNKCTNACSLLCRAGTHNGHMNPRCRTEQAAATQLPTCRVQLLLNYHVEGRADP
  GRVPAAGGRPEEGDEPDAEGDGPWPEAGDPRGEVGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVKTKHQM
  YSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDLDKGILLNWTKGFKASGAE
  GNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDRQCEVGMIVGTGCNACYMEEMQNVELVEGD
  EGRMCVNTEWGAFGDSGELDEFLLEYDRMVDESSANPGQQLYEKIIGGKYMGELVRLVLLKLVDENLLFH
  GEASEQLRTRGAFETRFVSQVESDSGDRKQIHNILSTLGLRPSVTDCDIVRRACESVSTRAAHMCSAGLA
  GVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEITFIESEEGSGRGAALVSAVAC
  KKACMLAQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270849
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270849.1, NP_001257778.1
• RefSeq Size: 2442
• RefSeq ORF: 1497
• Locus ID: 24385
• MW: 54.8 kDa
• Gene Summary: catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006]