Ears2 (NM_001159493) Rat Tagged ORF Clone

CAT#: RR216839

Ears2 (myc-DDK-tagged) - Rat glutamyl-tRNA synthetase 2, mitochondrial (Ears2)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ears2
• Synonyms: RGD1307904
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216839 representing NM_001159493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTCCGCAAGAGCCTTCTGCTGGCCGGGCCTCACGTGGGGGCCCTCGGGCACCGCGTGGGTC
  GGCGCGAGGCCAGTCTGGGCCCAGATCCCGGGGTCACGGTGCGAGTGCGCTTCGCTCCCAGCCCCACAGG
  CTTCTTACACCTGGGTGGCCTCCGTACTGCCCTCTACAACTACATCTTTGCCAAGAAGCACCAAGGGAGC
  TTCATTCTGAGGCTGGAGGACACTGATCAGAGCCGCCTGGTACCGGGGGCAGCAGAAAACATTGAGGACA
  TGCTGGAGTGGGCAGGCATCCCACCTGATGAAAGTCCCCGCCAGGGTGGTCCTGCAGGGCCCTACTATCA
  GTCACAGCGGCTAGCTCTGTATGCCCAGGCCACAGAAGCATTGCTGAAATCTGGAGCTGCCTATCCCTGT
  TTCTGTTCACCACAGCGGCTGGAGCTCCTGAAGAAGGAAGCACTGAGGAGCCGTCAGACACCTCGGTATG
  ACAATCGATGCCGGAACCTGAGCCAGGCACAGGTGGCCCAGAAGCTGGCCACAGACCCCAAGCCTGCTAT
  CCGCTTTCGCCTAGAGGAAGCAGTACCAGCCTTCCAAGACCTGGTATATGGCTGGACTCAGCATGAAGTG
  GCCAGTGTGGAGGGGGACCCAGTTATCCTGAAAAGTGATGGCTTTCCTACCTACCACCTAGCCTGTGTGG
  TAGATGACCACCACATGAGCATCAGCCATGTACTTCGGGGCTCTGAGTGGCTGGTCTCTACATCCAAGCA
  CTTGCTCCTCTACCAGGCCCTGGGTTGGCAGCCACCTCAATTTGCTCATCTGCCCCTGCTCCTCAACAGG
  GATGGTAGCAAGCTGTCTAAGAGGCAAGGGGACATTTTCCTGGAACATTTCGCTGCCACCGGTTTTCTTC
  CAGAAGCCTTGCTTGACATCATCACCAACTGTGGCTCGGGGTTTGCAGAGAACCAAATGGGCAGGACCCT
  GCCGGAGCTGATTACACAGTTTGACCTGACTCGGATCACCTGCCACTCAGCCCTGCTGGACCTAGAGAAG
  CTCCCAGAATTCAACAGGCTGCACCTCCGCCGGCTGGTGAGCAGTGAGACCCAGAGGCCCCATTTGGTGG
  AGAAGTTGCAGGGCCTGGTGAAGGAGGCATTTGGGAGCGAACTGCAAGACAGGGATGTTCTGGACCCAGC
  ATATATGGAGAGGATCCTCCTGCTGCGACAGGGTCACATCAGCCGTCTGCAGGATCTGGTCTCCCCAGTG
  TACTCCTACTTATGGACCCGCCCTGCTGTCCATCGAGCAGAGCTGGGCGCCAGCTCGGAAAAGTTGGATG
  TGATTGCCAAACACTTGCTGGGGCTTTTAGAAAGACCTGGTTTAAGCCTAACTCAGGATGTGGTGAACAG
  AGAACTGAAGAAGTTGTCAGAAGGCCTGGAAGGCACCAAGCATAGCAGCGTGATGAAGCTCCTCCGGATG
  GCCCTCAGTGGACAGCTGCAAGGACCCCCTGTGGCTGAGATGATGGTGTCCTTGGGCCCAGAGGAAGTAC
  GGGAACGAATCCAGAAGGTGCTTTCTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216839 representing NM_001159493
  Red=Cloning site Green=Tags(s)
  
  MAALRKSLLLAGPHVGALGHRVGRREASLGPDPGVTVRVRFAPSPTGFLHLGGLRTALYNYIFAKKHQGS
  FILRLEDTDQSRLVPGAAENIEDMLEWAGIPPDESPRQGGPAGPYYQSQRLALYAQATEALLKSGAAYPC
  FCSPQRLELLKKEALRSRQTPRYDNRCRNLSQAQVAQKLATDPKPAIRFRLEEAVPAFQDLVYGWTQHEV
  ASVEGDPVILKSDGFPTYHLACVVDDHHMSISHVLRGSEWLVSTSKHLLLYQALGWQPPQFAHLPLLLNR
  DGSKLSKRQGDIFLEHFAATGFLPEALLDIITNCGSGFAENQMGRTLPELITQFDLTRITCHSALLDLEK
  LPEFNRLHLRRLVSSETQRPHLVEKLQGLVKEAFGSELQDRDVLDPAYMERILLLRQGHISRLQDLVSPV
  YSYLWTRPAVHRAELGASSEKLDVIAKHLLGLLERPGLSLTQDVVNRELKKLSEGLEGTKHSSVMKLLRM
  ALSGQLQGPPVAEMMVSLGPEEVRERIQKVLSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159493
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001159493.1, NP_001152965.1
• RefSeq Size: 1961
• RefSeq ORF: 1572
• Locus ID: 361641
• MW: 58.3 kDa