Pole2 (NM_001169108) Rat Tagged ORF Clone

CAT#: RR216854

Pole2 (myc-DDK-tagged) - Rat polymerase (DNA directed), epsilon 2, accessory subunit (Pole2)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pole2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216854 representing NM_001169108
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGAGCGGCTGCGGAGCCGCACGGTCTCAGCTTTCAAGCTTCGGGGCTTGCTGCTCAGGAGTG
  AAGCCACTAAGTATCTCACTGAAGCTCTCCAGTCTGTCACTGAATTGGAGCTCGAGGAGAACCTAGAAAA
  GATCATTGATGCAGTTGAAAAGCAGCCCTTGTCATCAAACATGATCGAACGATCTGTAGTGGAAGCAGCT
  GTCCAGGAGTGCAGTCAGTCTGTTGATGAGACTATAGAACATATTTTCAATATCATTGGAGCTTTTGATA
  TTCCACATTTTATATATAATTCAGAAAGAAAAAAGTTTCTTCCTCTATCAATGACTAACCATGCTGCACC
  AACCATATTTGGAACAGCAAGGGACAAAGCCGAGCTGTACCGTGAACGATACACCATTTTACACCAGAGG
  ACGCACAGACATGAATTGTTCACTCCTCCAGTGATAGGCTCACACCCCGACGAAAGTGGAAGCAAATTCC
  AGCTTAAAACAATAGAAACTTTGCTGGGTAGTACGAGTAAAGTTGGTGACGTGATTGTCCTTGGAATGAT
  AACCCAGTTGAAAGAGGGGAAGTTTTTCCTGGAAGACCCTACTGGAACTGTACAGCTGGACCTTAGTAAA
  GCTCAGTTCCACAGTGGCCTATACACTGAGGCATGCTTTGTGCTGGCCGAAGGTTGCTTTGAAGATCAAG
  TGTTTCATGCCAATGCCTTTGGATTTCCTCCCACGGAGCCCTCTAGCACCACAAGGGCATACTATGGAAA
  TATTAATTTTTTTGGAGGGCCTTCTAATGCGTCAGTGAAGGCTTCTGCAAAACTGAGACAGCTGGAAGAG
  GAGAACAAGGACGCCATGTTTGTGATTGTGTCCGACGTTTGGTTGGATCAAGTGCAAGTGCTGGAGAAGC
  TTCACGTAATGTTTTCTGGTTATTCCCCTGCACCTCCGACCTGCTTTATACTGTGCGGTAACTTTTCATC
  TGCACCATATGGAAAAAATCAAGTCCAGGCTTTGAAAGATTCCCTAAAATCTTTGGCAGACATAATATGT
  GAATACCCAAGTATCCACCAAAGTAGCCGTTTTGTGTTTGTGCCTGGCCCGGAGGATCCTGGATTTGGTT
  CTATTTTACCAAGGCCGCCCCTTGCTGAAAGCATCACACAAGAATTCAGACAAAGGGTCCCATTTTCTGT
  TTTCACTACTAACCCTTGCAGAATTCAGTATTGTACACAAGAAATAATTATCTTCCGTGAAGACTTAGTG
  AATAAAATGTGTAGAAACTGTGTCCGCTTTCCTAGCAGCAGTTTGGATATTCCTACCCATTTCGTAAAGA
  CTGTCTTATCTCAGGGACATCTGGCTCCTCTCCCTCTTTACGTGTCCCCCGTCTACTGGGCATATGACTA
  CACTTTGAGAGTGTACCCAGTGCCCGATCTGCTGGTCATTGCAGACAAGTACGATCCGTTCACCGTGACC
  AACACCGAGTGCCTCTGCATAAACCCTGGCTCCTTTCCAAGAAGTGGATTTGCATTTAAAGTTTTTTACC
  CTTCCAGTAAGACAGTAGAAGACAGCAAACTTCAGGGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216854 representing NM_001169108
  Red=Cloning site Green=Tags(s)
  
  MAPERLRSRTVSAFKLRGLLLRSEATKYLTEALQSVTELELEENLEKIIDAVEKQPLSSNMIERSVVEAA
  VQECSQSVDETIEHIFNIIGAFDIPHFIYNSERKKFLPLSMTNHAAPTIFGTARDKAELYRERYTILHQR
  THRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSK
  AQFHSGLYTEACFVLAEGCFEDQVFHANAFGFPPTEPSSTTRAYYGNINFFGGPSNASVKASAKLRQLEE
  ENKDAMFVIVSDVWLDQVQVLEKLHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIIC
  EYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLV
  NKMCRNCVRFPSSSLDIPTHFVKTVLSQGHLAPLPLYVSPVYWAYDYTLRVYPVPDLLVIADKYDPFTVT
  NTECLCINPGSFPRSGFAFKVFYPSSKTVEDSKLQGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001169108
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001169108.1, NP_001162579.1
• RefSeq Size: 1696
• RefSeq ORF: 1584
• Locus ID: 299112
• MW: 59.2 kDa