Shc1 (NM_001164060) Rat Tagged ORF Clone

CAT#: RR216944

Shc1 (myc-DDK-tagged) - Rat SHC (Src homology 2 domain containing) transforming protein 1 (Shc1), transcript variant 1

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shc1
• Synonyms: P66shc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216944 representing NM_001164060
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTTCTACCCCCCAAGCCGAAGTACAACCCACTTCGGAATGAGTCTCTGTCATCGCTGGAGGAGG
  GGGCTTCAGGGTCTACTCCACCGGAGGAGCTGCCTTCCCCATCAGCCTCATCCCTGGGACCCATTCTGCC
  TCCTCTGCCGGGGGACGATAGCCCGACTACCCTGTGTTCCTTCTTTCCCCGGATGAGCAACTTGAAGCTG
  GCCAATCCTGCTGGGGGGCGCCTGGGACCTAAAGGGGAGCCAGGAAAAGCTGCAGAAGATGGGGAAGGGA
  GTGCAGGAGCAGCCCTTCGGGACTCAGGCCTCTTGCCCCTCCTCCAGGACATGAACAAGCTGAGTGGAGG
  CGGCGGGCGCAGGACTCGGGTAGAAGGGGGCCAGCTGGGGGGCGAGGAGTGGACCAGACACGGGAGCTTT
  GTCAATAAGCCCACACGAGGCTGGCTGCATCCCAACGACAAAGTCATGGGACCTGGGGTTTCCTACTTGG
  TTCGGTACATGGGTTGCGTGGAGGTCTTACAGTCAATGCGAGCCCTTGACTTCAATACCCGGACTCAGGT
  CACCAGGGAGGCTATCAGTTTGGTGTGTGAAGCTGTGCCTGGTGCCAAAGGGGCGATGAGGAGGAGAAAG
  CCCTGCAGCCGCCCACTCAGCTCTATCCTGGGAAGGAGTAACCTGAAGTTTGCTGGAATGCCGATCACTC
  TCACTGTGTCTACCAGCAGCCTTAACCTCATGGCAGCAGACTGCAAACAGATCATTGCCAACCATCACAT
  GCAATCTATCTCATTCGCGTCTGGTGGGGATCCGGACACAGCTGAGTACGTTGCCTATGTTGCCAAAGAC
  CCTGTGAATCAGAGAGCCTGCCATATCCTGGAGTGTCCTGAAGGACTTGCTCAGGACGTCATCAGCACCA
  TCGGGCAGGCCTTTGAGTTGCGCTTCAAACAGTATCTCAGGAATCCACCCAAGCTGGTCACCCCCCATGA
  CAGGATGGCTGGCTTTGATGGCTCAGCTTGGGATGAGGAGGAAGAAGAGCCCCCTGACCATCAGTACTAC
  AATGACTTCCCAGGGAAGGAACCCCCTCTTGGTGGAGTGGTAGATATGAGGCTTCGGGAAGGGGCTGCTC
  GACCCACTCTGCCCAGTACCCAGATGCCCAGCCACTTGGGAGCTACACTGCCAATAGGGCAGCATGTTAC
  AGGAGACCATGAAGTCCGTAAACAGATGCTGCCTCCACCACCGTGCCCAGGCAGAGAACTCTTCGATGAT
  CCTTCCTATGTCAACATCCAGAATCTAGACAAGGCCCGGCAAGCTGGTGGTGGGGCTGGACCCCCAAATC
  CTTCTGTTAATGGCAGTGCACCCCGAGACCTCTTTGACATGAAGCCCTTCGAAGATGCACTCCGGGTGCC
  GCCAGCTCCCCAGTCCATGTCCATGGCTGAGCAGCTCCAAGGGGAGTCCTGGTTTCATGGGAAACTCAGC
  CGGCGGGAGGCTGAGGCGCTGCTGCAGCTCAACGGTGACTTCTTGGTGCGAGAGAGTACAACCACACCTG
  GCCAGTACGTGCTCACTGGTCTGCAGAGTGGGCAGCCCAAGCACTTGCTTCTGGTGGACCCTGAAGGTGT
  GGTTCGGACAAAGGATCACCGCTTTGAGAGTGTCAGTCACCTGATCAGCTACCACATGGACAATCACTTA
  CCCATCATCTCTGCAGGCAGTGAGCTGTGTCTACAGCAACCTGTGGATCGAAAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216944 representing NM_001164060
  Red=Cloning site Green=Tags(s)
  
  MDLLPPKPKYNPLRNESLSSLEEGASGSTPPEELPSPSASSLGPILPPLPGDDSPTTLCSFFPRMSNLKL
  ANPAGGRLGPKGEPGKAAEDGEGSAGAALRDSGLLPLLQDMNKLSGGGGRRTRVEGGQLGGEEWTRHGSF
  VNKPTRGWLHPNDKVMGPGVSYLVRYMGCVEVLQSMRALDFNTRTQVTREAISLVCEAVPGAKGAMRRRK
  PCSRPLSSILGRSNLKFAGMPITLTVSTSSLNLMAADCKQIIANHHMQSISFASGGDPDTAEYVAYVAKD
  PVNQRACHILECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQYY
  NDFPGKEPPLGGVVDMRLREGAARPTLPSTQMPSHLGATLPIGQHVTGDHEVRKQMLPPPPCPGRELFDD
  PSYVNIQNLDKARQAGGGAGPPNPSVNGSAPRDLFDMKPFEDALRVPPAPQSMSMAEQLQGESWFHGKLS
  RREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLISYHMDNHL
  PIISAGSELCLQQPVDRKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164060
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164060.1, NP_001157532.1
• RefSeq Size: 3541
• RefSeq ORF: 1740
• Locus ID: 85385
• MW: 62.7 kDa
• Gene Summary: mouse homolog interacts with growth factor receptors; involved in transformation and differentiation in a Ras-dependent fashion [RGD, Feb 2006]