Fpgs (NM_001146125) Rat Tagged ORF Clone

CAT#: RR216958

Fpgs (myc-DDK-tagged) - Rat folylpolyglutamate synthase (Fpgs)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fpgs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216958 representing NM_001146125
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTGGGCGCGGAGCCACCTGTGCTCGGCTCTATCCTTGGCAGCTGTTTCTGCGCGTGGTGCAACGA
  TGGAGGGCGCGGCGTATCGGTGGTTGAGCGCGTGGCCAGCGCCGCAGGAGCCGAGCATGGAGTATCAGGA
  TGCTGTGCGCACGCTCAACACCCTGCAAACCAATGCCAGCTGCCTGGAGGAGGTAAAGCGTCAACGGAGT
  GACCCTCAGGCGCAGCTAGAAGCTATGGAGGTGTACCTCGCACGGAGTGGACTGCAGGTGGAGGATTTGA
  ACCAACTAAAAATTATCCATGTCACTGGGACCAAAGGAAAGGGCTCCACCTGTGCCTTCACTGAACGGAT
  CCTGCGGAATTACGGCCTGAAAACTGGCTTCTTTAGCTCTCCCCACCTGGTGCAGGTGCGTGAGCGGATT
  CGAATCAACGGGAAGCCCATCAGCCCCGAACTCTTTACCAAGTACTTCTGGCACCTCTATCAGCAGCTGG
  AGGAATTCAAGGATGACAGCCACATTTCCATGCCCTCTTACTTCCGCTTCCTCACACTCATGGCTTTCCA
  TGTCTTCCTCCAAGAGAAGGTGGACCTGGCCGTGGTGGAAGTGGGCATTGGTGGGGCTTTTGACTGCACC
  AACATCATCAGAAAGCCAGTGGTGTGTGGAGTCTCCTCTCTTGGCATTGACCACACCAGTCTGCTAGGAG
  ATACAGTGGAGAAAATAGCATGGCAGAAAGGGGGCATCTTTAAGCCTGGTGTCCCTGCCTTCACTGTGCT
  ACAGCCAGAAGGTCCCCTGGCCGTGCTGAGGGATAGAGCCCAGCAGATCTCATGCCCCCTGTACCTGTGT
  CCACCATTGGAAGCCCTGGAGCAGGTTGGATTGCCATTGAGCCTGGGCCTGGAGGGAGAGCACCAGCGGC
  ATAATGCAGCCTTGGCCTTGCAGCTGGCCCGTTGTTGGCTGGAGCAGCAGAACTACCATGACATCCAGGA
  GCTGAAGGCATCCAAGCCAAGCATTCGGTGGCAGCTGCCGCTGGCACCTGTGTTCCGCCCCACGCCCCAC
  ATGAGACGTGGGCTTCGGGACACAGAGTGGCCTGGTCGGACACAGGTACTCCAGCGGGGACCTGTTACCT
  GGTACCTGGATGGCGCTCATACCACCAGCAGTGTGCAGGCCTGTGTGCACTGGTACTGCCAGTCACTGGA
  GCGCAGCAGACGCACCGACGGTGGGCATGAAGTACACATCTTGCTCTTCAACTCCACTGGTGACAGGGAC
  TCTGCTGCCCTACTGAAGTTGCTGCAGCCCTGCCATTTTGATTACGCTGTCTTCTGCCCCAATGTGACAG
  AGATTTCATCCATAGGAAACGCAGACCAGCAGAACTTCACGGTGACTCTGGACCAAGTGCTCCTCCGCTG
  CCTGCAGCACCAGAAGCATTGGAACTGCCTGGCAGAGAAACAGGCCAGCTCCAACCTCTGGGGCAGCTGT
  GGCCCAGAGTCTGCCCGGCCAGGCTCCCTGCTGCTGGCACCGCACCCACCTCAGCCCACTAGCACGAGCT
  CCCTTGTTTTCAGCTGCATCTCCCACGCCTTGCAGTGGATCAGCCAAGGCCGGGATCCCATCTTTCAGCC
  CGAGAGCCCTCCAAGGAACCTTCTGAACCACCCCACGGCCAACAGCGGGGCGAGCATTCTCCGAGAGGCC
  GCTGCCATCCACGTACTGGTCACGGGGAGCCTGCACCTGGTGGGTGGGGCGCTGAGGCTGCTTGATCCCT
  CCATGTCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216958 representing NM_001146125
  Red=Cloning site Green=Tags(s)
  
  MSWARSHLCSALSLAAVSARGATMEGAAYRWLSAWPAPQEPSMEYQDAVRTLNTLQTNASCLEEVKRQRS
  DPQAQLEAMEVYLARSGLQVEDLNQLKIIHVTGTKGKGSTCAFTERILRNYGLKTGFFSSPHLVQVRERI
  RINGKPISPELFTKYFWHLYQQLEEFKDDSHISMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCT
  NIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVLQPEGPLAVLRDRAQQISCPLYLC
  PPLEALEQVGLPLSLGLEGEHQRHNAALALQLARCWLEQQNYHDIQELKASKPSIRWQLPLAPVFRPTPH
  MRRGLRDTEWPGRTQVLQRGPVTWYLDGAHTTSSVQACVHWYCQSLERSRRTDGGHEVHILLFNSTGDRD
  SAALLKLLQPCHFDYAVFCPNVTEISSIGNADQQNFTVTLDQVLLRCLQHQKHWNCLAEKQASSNLWGSC
  GPESARPGSLLLAPHPPQPTSTSSLVFSCISHALQWISQGRDPIFQPESPPRNLLNHPTANSGASILREA
  AAIHVLVTGSLHLVGGALRLLDPSMSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146125
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146125.2, NP_001139597.2
• RefSeq Size: 2586
• RefSeq ORF: 1764
• Locus ID: 687266
• MW: 65.3 kDa
• Gene Summary: human homolog catalyzes an ATP-dependent ligation step in the synthesis of poly(gamma-glutamate) folate metabolites [RGD, Feb 2006]