Aifm3 (NM_001013977) Rat Tagged ORF Clone

CAT#: RR216986

Aifm3 (myc-DDK-tagged) - Rat apoptosis-inducing factor, mitochondrion-associated 3 (Aifm3)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aifm3
• Synonyms: RGD1306028
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216986 representing NM_001013977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGCTGCTTCTCCAAGCCCAAGCCAGTGGAGCTCAAGATCGAAGTGGTGCTGCCAGAGAAAGAGC
  GGGGCAAGGAAGAGCTGTCAGCCAGCGGAAAGGGCAGCCCCCGGGGTTACCAGGGCAATGGCACAGCACG
  TCACTTCCATGCTGAAGAGCGTCTGCCCACTCCACAGCCCTACCCCAGCCCTCAGGACTGTGTGGAGGCT
  ACCGTCTGCCATGTCAAGGACCTGGAGAATGGCCAGATGCGGGAAGTGGAGTTGGGCTGGGGAAAGGTGT
  TGCTGGTGAAGGATAATGGGGAATTCCACGCCCTGGGCCATAAGTGTCCTCACTACGGAGCACCCCTGGT
  GAAAGGTGTGCTGTCCCGGGGACGTGTGCGCTGCCCCTGGCATGGTGCCTGCTTCAATATTGGAACTGGG
  GACCTAGAGGACTTCCCAGGCCTGGACAGTCTGCATAAGTTCCAGGTGAAGATTGAGAAGGAGAAGGTGA
  CCGTTCGGGCAAGCAAGCAGGCCCTGCAGCTGCAGCGGAGGACAAAGGTGATGGCCAAGTGCATCTCTCC
  AAGTGCTGGCCACAGCAGCACCAATGTGCTCATAGTGGGCGCAGGTGCTGCTGGCCTAGTGTGCGCAGAG
  ACACTGAGGCAGGAGGGCTTCTCAGACCGCATTGTCCTTTGCACACTGGATCGACATCTGCCTTATGATC
  GGGCCAAGCTCAGCAAGTCCCTGGATGCACAACCTGAACAGCTGGCCCTGAGACCCAAGGAATTCTTCCG
  GGCCTATGGCATCGAGATGCTCACTGAAGCTCAGGTGGTCACAGTGGACGTGAGAAATAAGAAGGTTGTG
  TTCAAGGACGGCTTCAAGCTAGAGTACAGCAAGCTGCTGCTGGCACCAGGAAGTAGCCCTAAGACCCTGA
  CCTGCAAAGGGAAAGACATAGAGAATGTGTTCACCATCCGCACGCCTGAGGATGCCAACCGCGTGCTGCG
  GCTGGCCCGGGGCCGAAATGCTGTTGTTGTGGGAGCAGGCTTTCTGGGGATGGAGGTCGCTGCTTATCTG
  ACTGAAAAAGCCCACTCAGTATCTGTGGTAGAGCTGGAGGAGACGCCCTTCCGCAGGTTCCTGGGGGAGC
  GTGTTGGTCGTGCCCTTATGAAGATGTTTGAAAACAACCGGGTGAAGTTCTATATGCAGACAGAGGTGTC
  AGAGCTGCGGGCTCAAGAGGGCAAGCTGCAGGAGGTGGTGCTGAAGAGCAGCAAAGTTCTGCGGGCAGAC
  GTCTGCGTGGTAGGCATCGGTGCGGTGCCTGCCACAGGCTTCCTGAGGCAGAGTGGCATTGGTCTGGATT
  CCCGAGGTTTCATCCCAGTCAACAAGATGATGCAGACCAACATCCCAGGAGTGTTTGCTGCAGGTGATGC
  TGTCACCTTTCCTCTTGCCTGGAGGAACAATCGGAAAGTGAACATCCCACACTGGCAGATGGCTCATGCC
  CAGGGTCGGGTAGCAGCTCAGAACATGCTGGCACAGGAGGCAGAGATCAACACGGTACCTTATCTGTGGA
  CTGCCATGTTCGGCAAGAGCCTGCGCTATGCTGGCTACGGAGAAGGCTTCGATGATGTCATCATTCAGGG
  GGATCTGGAGGAGCTGAAGTTTGTGGCTTTTTATACCAAAGGTGACGAAGTGATTGCTGTGGCCAGCATG
  AACTACGACCCCATCGTATCCAAGGTGGCCGAGGTGCTGGCCTCAGGTCGAGCCATCCGGAAGCGGGAGG
  TGGAGTTGTTTATGCTGCACAGCAAGACCGGTGACATGTCTTGGCTCACAGGGAAAGGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216986 representing NM_001013977
  Red=Cloning site Green=Tags(s)
  
  MGGCFSKPKPVELKIEVVLPEKERGKEELSASGKGSPRGYQGNGTARHFHAEERLPTPQPYPSPQDCVEA
  TVCHVKDLENGQMREVELGWGKVLLVKDNGEFHALGHKCPHYGAPLVKGVLSRGRVRCPWHGACFNIGTG
  DLEDFPGLDSLHKFQVKIEKEKVTVRASKQALQLQRRTKVMAKCISPSAGHSSTNVLIVGAGAAGLVCAE
  TLRQEGFSDRIVLCTLDRHLPYDRAKLSKSLDAQPEQLALRPKEFFRAYGIEMLTEAQVVTVDVRNKKVV
  FKDGFKLEYSKLLLAPGSSPKTLTCKGKDIENVFTIRTPEDANRVLRLARGRNAVVVGAGFLGMEVAAYL
  TEKAHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRAQEGKLQEVVLKSSKVLRAD
  VCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNIPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHA
  QGRVAAQNMLAQEAEINTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASM
  NYDPIVSKVAEVLASGRAIRKREVELFMLHSKTGDMSWLTGKGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013977
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013977.2, NM_001013977.3, NP_001013999.2
• RefSeq Size: 2314 bp
• RefSeq ORF: 1815 bp
• Locus ID: 303786
• Cytogenetics: 11q23
• MW: 66.6 kDa