Chat (NM_001170593) Rat Tagged ORF Clone

CAT#: RR217035

Chat (myc-DDK-tagged) - Rat choline O-acetyltransferase (Chat)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217035 representing NM_001170593
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATCCTGGAAAAGGCTCCCCAAAAGATGCCTGTAAAGGCTTCTAGCTGGGAGGAGCTGGACTTAC
  CTAAATTACCAGTGCCCCCGCTGCAGCAAACCCTGGCCACCTACCTACAGTGCATGCAGCATCTGGTACC
  TGAAGAGCAGTTCAGGAAGAGCCAGGCCATTGTGAAGCGGTTTGGGGCCCCTGGTGGCCTGGGTGAGACC
  CTGCAGGAAAAGCTCTTGGAGAGACAGGAGAAGACAGCCAATTGGGTCTCTGAATACTGGCTGAATGACA
  TGTACCTGAACAACCGCCTGGCCCTGCCAGTCAACTCCAGCCCTGCTGTGATCTTTGCTCGGCAGCACTT
  CCAAGACACCAATGACCAGCTAAGATTTGCAGCCTGCCTCATCTCTGGTGTGCTTAGCTACAAAACTCTG
  CTGGACAGCCACTCCCTTCCCACTGACTGGGCCAAGGGGCAGCTCTCAGGGCAGCCCCTCTGTATGAAGC
  AATATTACAGACTCTTCTCCTCTTACCGGCTTCCTGGCCATACCCAGGACACACTGGTGGCCCAGAAGAG
  CAGTATCATGCCCGAGCCTGAGCATGTCATCGTGGCCTGCTGCAACCAGTTCTTTGTCTTGGATGTTGTC
  ATTAATTTCCGCCGTCTCAGTGAGGGTGATCTGTTCACTCAGTTGAGAAAGATAGTCAAAATGGCGTCCA
  ACGAGGATGAACGCCTGCCTCCAATCGGCCTGCTGACGTCAGACGGGAGGAGCGAGTGGGCCAAGGCCAG
  GACGGTCCTCTTAAAAGACTCCACCAACCGGGACTCCCTGGACATGATCGAGCGCTGCATCTGCCTGGTA
  TGCCTGGATGGTCCAGGCACAGGAGAGCTCAGTGACACCCACAGGGCCCTCCAACTCCTTCACGGTGGAG
  GCTGCAGCCTGAACGGAGCCAATCGCTGGTATGACAAGTCCCTGCAGTTCGTGGTGGGCCGAGATGGCAC
  CTGCGGTGTGGTGTGTGAGCATTCTCCATTTGATGGCATCGTCCTGGTGCAGTGCACAGAGCACCTGCTG
  AAACATATGATGACAAGCAACAAGAAGCTCGTCAGGGCTGACTCAGTGAGTGAGCTCCCTGCCCCCAGAA
  GGCTGAGGTGGAAATGTTCCCCAGAAACCCAAGGCCATCTCGCCTCCTCAGCAGAGAAACTTCAAAGAAT
  AGTAAAGAATCTGGATTTCATTGTTTACAAGTTTGACAACTATGGAAAAACATTTATCAAGAAGCAGAAA
  TACAGCCCCGACGGCTTCATCCAGGTGGCCCTCCAGCTGGCTTACTACAGGCTTTACCAGAGACTGGTGC
  CCACCTATGAGAGCGCATCCATCCGCCGCTTCCAGGAAGGTCGGGTGGACAACATCAGATCAGCCACTCC
  AGAGGCTCTGGCTTTTGTGCAAGCCATGACTGACCACAAGGCTGCCATGCCGGCTTCTGAGAAACTGCAG
  CTGCTGCAGACAGCCATGCAGGCCCAGACTGAGTACACAGTCATGGCCATAACCGGCATGGCCATTGACA
  ACCATCTTCTGGCACTGAGGGAGCTGGCCCGAGACCTGTGCAAAGAGCCACCTGAGATGTTCATGGATGA
  AACATACCTGATGAGCAACCGGTTTGTCCTCTCCACCAGCCAGGTGCCCACAACCATGGAGATGTTCTGC
  TGTTATGGACCCGTGGTCCCCAATGGCTATGGAGCCTGCTACAACCCCCAGCCCGAGGCCATCACCTTCT
  GCATCTCCAGTTTTCACAGCTGCAAAGAGACCTCGTCTGTGGAGTTTGCGGAAGCTGTGGGAGCGAGCCT
  TGTTGACATGAGAGACCTCTGCAGTTCAAGGCAGCCTGCTGACAGCAAGCCACCAGCACCCAAGGAAAAA
  GCTAGGGGCCCAACCAAGCCAAGCAATCTTGACTCCAGCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217035 representing NM_001170593
  Red=Cloning site Green=Tags(s)
  
  MPILEKAPQKMPVKASSWEELDLPKLPVPPLQQTLATYLQCMQHLVPEEQFRKSQAIVKRFGAPGGLGET
  LQEKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFQDTNDQLRFAACLISGVLSYKTL
  LDSHSLPTDWAKGQLSGQPLCMKQYYRLFSSYRLPGHTQDTLVAQKSSIMPEPEHVIVACCNQFFVLDVV
  INFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAKARTVLLKDSTNRDSLDMIERCICLV
  CLDGPGTGELSDTHRALQLLHGGGCSLNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLL
  KHMMTSNKKLVRADSVSELPAPRRLRWKCSPETQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQK
  YSPDGFIQVALQLAYYRLYQRLVPTYESASIRRFQEGRVDNIRSATPEALAFVQAMTDHKAAMPASEKLQ
  LLQTAMQAQTEYTVMAITGMAIDNHLLALRELARDLCKEPPEMFMDETYLMSNRFVLSTSQVPTTMEMFC
  CYGPVVPNGYGACYNPQPEAITFCISSFHSCKETSSVEFAEAVGASLVDMRDLCSSRQPADSKPPAPKEK
  ARGPTKPSNLDSSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170593
• ORF Size: 1932 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170593.1, NP_001164064.1
• RefSeq Size: 3599
• RefSeq ORF: 1935
• Locus ID: 290567
• MW: 72.4 kDa