Gars (NM_001271139) Rat Tagged ORF Clone

CAT#: RR217147

Gars (myc-DDK-tagged) - Rat glycyl-tRNA synthetase (Gars)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gars
• Synonyms: GlyRS; RGD1559871
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217147 representing NM_001271139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGTCTGCTCCCCACGCTGCTCAGAGCCACCCGCGCCGCGCTGCTCCTACAGTCGCCCCGAGTGG
  TCGCAGCGCCCGCGTCTCAGCGGCTCCTCAGCGCTCCCGCGCAGCCCGCCGCCTCTCCGAGCAGCATGGA
  CAGCGCCGAGGAGCTGCTAGCCCCATTGCGGCTAGCTGTGCGCCAGCAGGGAGACTTTGTACGGAAGTTA
  AAAGAAGATAAAGCCCCACAAGTGGATGTGGACAGAGCAGTAGCTGAGCTCAAGGCCCGGAAGAGGGTTC
  TGGAAGCAAAGGAGCTGGCGTTGCAGCCCAAAGATGACATCGTAGATAGAGCAAAAATGGAGGATACTTT
  GAAGAGGAGATTTTTCTACGATCAAGCTTTTGCTATTTATGGAGGTGTCAGTGGTTTGTATGACTTTGGG
  CCAGTGGGATGTGCTTTGAAGAACAATATCATCCAGACCTGGAGGCAGCACTTTATCCAAGAGGAACAGA
  TCCTGGAAATTGACTGCACCATGCTCACCCCCGAGCCAGTTTTAAAGACCTCTGGCCATGTAGACAAATT
  TGCTGACTTCATGGTGAAGGACGTGAAGAACGGAGAGTGCTTCCGCGCGGACCACCTGTTGAAAGCTCAT
  TTACAGAAACTGATGTCAGATAAGAAGTGCTCTGCTGAGAAGAAATCAGAGATGGAAAGTGTCTTGGCCC
  AGCTTGATAACTATGGACAACAAGAACTGGCGGATCTTTTTGTGAACTATAATGTAAAATCTCCCACCAC
  TGGCAATGACCTGTCCCCTCCGGTACCTTTTAACTTAATGTTCCAGACCTTCATTGGGCCTGGAGGAAAC
  ATGCCTGGATATCTGAGACCAGAAACTGCACAGGGAATTTTCCTAAATTTCAAACGACTTTTGGAATTCA
  ACCAAGGGAAGTTGCCTTTTGCTGCTGCACAGATTGGAAACTCCTTTAGAAATGAGATCTCGCCCCGGTC
  TGGACTGATCCGAGTCAGGGAATTCACAATGGCAGAGATCGAGCACTTTGTAGATCCCACTGAGAAAGAC
  CATCCCAAGTTCCCGAGCGTGGCCGACCTCTACCTTTATTTGTACTCTGCCAAAGCCCAGGTCACTGGAC
  AGTCTGCTCGCAAGATGCGTCTGGGAGATGCTGTTGAACAGGGTGTGATTAACAACTCAGTATTAGGCTA
  TTTTATTGGCCGCATCTACCTCTACCTCACGAAGGTTGGAATATCTCCTGATAAACTCCGCTTCCGACAG
  CATATGGAGAATGAGATGGCCCATTATGCCTGCGACTGCTGGGATGCTGAGTCCAAAACGTCCTATGGCT
  GGATCGAGATTGTTGGATGTGCTGACCGATCCTGCTATGACCTCTCCTGTCATGCTCGAGCCACCAAAGT
  CCCGCTGGTGGCTGAGAAGCCCCTGAAAGAGCCCAAAACAGTTAACGTTGTACAGTTTGAGCCCAACAAG
  GGAGCCGTGGGCAAGGCGTACAAGAAAGATGCAAAGCTGGTGCTGGAGTACCTCGGCGCCTGCGATGAGT
  GCTACATTACCGAGATGGAGCTGCTGCTGAGCGAGAAAGGGGAATTCACTATTGAAACTGAAGGAAAAAC
  ATTTCAGTTAACAAAAGACATGGTCAGTGTGAAGAGATTCCAGAAAACACTACACGTGGAAGAAGTTGTT
  CCGAGTGTAATTGAGCCCTCTTTTGGCCTGGGCAGGATCATGTATACCATATTGGAACATACATTCCACG
  TCCGGGAGGGAGATGAGCAGAGAACGTTCTTCAGTTTCCCTGCTGTGGTTGCTCCATTCAAATGTTCTGT
  CCTTCCACTGAGCCAAAACCAAGAGTTTATGCCATTTGTCAAGGAATTATCGGAAGCTCTGACTCGAAAT
  GGCGTGTCTCATAAAGTTGACGATTCCTCTGGGTCTATTGGAAGGCGCTATGCAAGAACTGATGAGATTG
  GCGTGGCGTTTGGCATCACCATTGACTTTGATACAGTGAACAAGACCCCCCACACTGCAACTCTGAGGGA
  CCGAGACTCCATGAGACAGATAAGGGCAGAGGTCTCTGAACTGCCCAGTGTGGTCCGTGATCTGGCCAAT
  GGCAACATCACTTGGGCTGATGTGGAGGCCAGGTACCCCCTCTTTGAAGGGCAAGAGACTGGCAAGAAGG
  AGACAGTGGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217147 representing NM_001271139
  Red=Cloning site Green=Tags(s)
  
  MPCLLPTLLRATRAALLLQSPRVVAAPASQRLLSAPAQPAASPSSMDSAEELLAPLRLAVRQQGDFVRKL
  KEDKAPQVDVDRAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFG
  PVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAH
  LQKLMSDKKCSAEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPTTGNDLSPPVPFNLMFQTFIGPGGN
  MPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPTEKD
  HPKFPSVADLYLYLYSAKAQVTGQSARKMRLGDAVEQGVINNSVLGYFIGRIYLYLTKVGISPDKLRFRQ
  HMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPNK
  GAVGKAYKKDAKLVLEYLGACDECYITEMELLLSEKGEFTIETEGKTFQLTKDMVSVKRFQKTLHVEEVV
  PSVIEPSFGLGRIMYTILEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRN
  GVSHKVDDSSGSIGRRYARTDEIGVAFGITIDFDTVNKTPHTATLRDRDSMRQIRAEVSELPSVVRDLAN
  GNITWADVEARYPLFEGQETGKKETVEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271139
• ORF Size: 2184 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271139.1, NM_001271139.2, NP_001258068.1
• RefSeq Size: 2459
• RefSeq ORF: 2187
• Locus ID: 297113
• MW: 81.8 kDa