Mapk7 (NM_001191547) Rat Tagged ORF Clone

CAT#: RR217226

Mapk7 (myc-DDK-tagged) - Rat mitogen-activated protein kinase 7 (Mapk7)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapk7
• Synonyms: Bmk1; Erk5; Prkm7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217226 representing NM_001191547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAACCGTTGAAGGAGGAGGACGGCGAAGATGGCTCTGGGGAACCCCCTGGGCGGGTGAAGGCAG
  AACCCGTCCACTCCGCTGCATCTGTCGTGGCCAAGAACCTGGCCCTGCTCAAAGCCCGCTCCTTCGACGT
  GACCTTTGACGTGGGGGACGAGTACGAGATCATCGAGACCATAGGCAATGGGGCCTACGGGGTGGTGTCC
  TCGGCGCGCCGCCGCCTCACGGGTCAGCAGGTGGCCATCAAGAAGATACCTAATGCTTTTGATGTGGTGA
  CCAATGCCAAACGGACCCTCAGGGAACTGAAGATCCTCAAACACTTCAAACACGACAATATCATCGCCAT
  CAAGGACATCCTGAGGCCCACTGTGCCCTATGGAGAATTCAGATCTGTCTACGTGGTACTGGACCTCATG
  GAAAGTGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACACTAGAACATGTGAGATACTTCCTAT
  ACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGCGATCTTAAACCCTCTAA
  CCTTTTGGTCAATGAGAACTGTGAGCTCAAGATTGGTGACTTTGGAATGGCCCGTGGCCTGTGTACATCC
  CCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCCACTCGCTGGTACCGTGCTCCTGAACTCATGC
  TTTCGCTGCATGAGTACACACAGGCTATTGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGC
  TCGGCGACAGCTCTTCCCAGGCAAAAACTATGTGCATCAGTTACAGCTGATCATGATGGTGTTGGGAACT
  CCATCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCACCAA
  GGCAGCCTGTACCTTGGGAGACAGTATACCCGGGTGCTGACCGCCAGGCCCTCTCACTGCTGGGACGCAT
  GTTGCGATTTGAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAGTAC
  CACGACCCTGACGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTACCA
  GGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCATGCACGACGGGAGGGCATCCGCCA
  ACAAATTCGCTTCCAGCCCTCTTTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTCGAGATGCCC
  AGCCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCACCTCCTCCAGCACTACCACCATGCTCTGGCC
  CTGCCCCTGACACTGTTGATCTGACTCTGCAGCCTGCCCCGCCCGCCAGTGAGCTTGCTCCGCCAAAAAG
  AGAGGGTGCCATCTCCGACAATACCAAAGCAGCCCTCAAAGCTGCTCTGCTCAAGTCCCTGAGGAGCCGG
  CTTCGAGATGGGCCCAGTGCACCCTTGGAGGCCCCTGAACCTCGAAAGCCTGTGACAGCTCAGGAACGTC
  AGCGAGAGCGAGAAGAGAAGCGCAGGAGGCGACAAGAGAGAGCCAAGGAGAGGGAGAAGCGACGACAAGA
  GCGGGAGCGCAAGGAGAGGGGGGCTGGCACCTTGGGGGGCCCCTCTACTGACCCTTTGGCTGGATTGGTG
  CTCAGTGACAATGACCGAAGCCTGCTAGAGCGGTGGACTCGCATGGCTAGGCCTCCTGTCCCTGCACCAG
  CACCAGCACCAGCACCAACACCAAAACCAACACCAACACCAACACAGTCCTCTGCTCAACCCACTAGTCC
  TCCCAATGGCCCTGTATCTCAGTCTACTGCTCCTCTACAGCCTGCAGGCTCTATTCCTGGTCCTGCCTCC
  CAGCCTGTTTGCCCACCCCCTGGGCCTGTTCCCCAGCCTGCTGGCCCTGTCCCTGCCCCTCTCCAGACTG
  CCCCTTCCACTAGCCTTTTGGCCTCCCAGTCACTTGTGCCACCTAGTGGGTTGCCTGGTTCTGGTGCCCC
  AGAAGTTCTGCCTTACTTCCCATCTGGCCCACCACCTCCAGATCCTGGGCTCACCCCTCAGCCTTCTACA
  TCAGAGTCACCTGATGTCAACCTGGTGACCCAGCAACTGTCCAAGTCTCAGGTGGAGGACCCCCTGCCTC
  CCGTGTTCTCAGGCACTCCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCTGGAGGAATTCTT
  AAACCAATCTTTTGACATGGGTGTGGCTGATGGGCCACAGGATAGCCAGGCAGACTCAGCCTCACTCTCA
  GCCTCCCTCCTTGCTGACTGGCTTGAGGGCCACGGCATGAACCCCGCTGACATTGAGTCTCTGCAGCGTG
  AGATCCAGATGGACTCCCCAATGTTGCTGTCTGACCTGCCTGACCTCCAAGAACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217226 representing NM_001191547
  Red=Cloning site Green=Tags(s)
  
  MAEPLKEEDGEDGSGEPPGRVKAEPVHSAASVVAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVS
  SARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFRSVYVVLDLM
  ESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTS
  PAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGT
  PSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKY
  HDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPVCPDVEMP
  SPWAPSGDCAMESPPPALPPCSGPAPDTVDLTLQPAPPASELAPPKREGAISDNTKAALKAALLKSLRSR
  LRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGTLGGPSTDPLAGLV
  LSDNDRSLLERWTRMARPPVPAPAPAPAPTPKPTPTPTQSSAQPTSPPNGPVSQSTAPLQPAGSIPGPAS
  QPVCPPPGPVPQPAGPVPAPLQTAPSTSLLASQSLVPPSGLPGSGAPEVLPYFPSGPPPPDPGLTPQPST
  SESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDSQADSASLS
  ASLLADWLEGHGMNPADIESLQREIQMDSPMLLSDLPDLQEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191547
• ORF Size: 2436 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001191547.1, NP_001178476.1
• RefSeq Size: 2738
• RefSeq ORF: 2439
• Locus ID: 114509
• MW: 88.5 kDa
• Gene Summary: binds and phosphorylates myocyte enhancer factor 2 (Mef2); may regulate Mef2 transcription activator function [RGD, Feb 2006]