Piwil4 (NM_001271133) Rat Tagged ORF Clone

CAT#: RR217245

Piwil4 (myc-DDK-tagged) - Rat piwi-like RNA-mediated gene silencing 4 (Piwil4)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Piwil4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217245 representing NM_001271133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGACGGGCCCGTGTGAGGGCGCGAGGCATCACCACTGGCCACAGTGCCAGGGAAGTGGAGCGGG
  CCCCAAGAGACCTCATGGTGAGTGCTTCTGTCTATGAGCTTGGCGTCTCTTCTGGTGATGGTAGACGTAC
  TCTCGTGGAAAAAAGAGGCAATGGCAGACAGGACTTCGTCGATTTGGGTGTCTGTACCAGGGAGAAGTTG
  ACTCACGTAAAAGATTCTAAAACAGGTTCTAGCGGAATACCTGTGCAGCTGGCTACAAATCTCTTCCATT
  TAGATTTGCCCCAGGATTGGCAGCTATACCAGTACCATGTCACATACAGCCCGGATTTAGCCTCTAGGAG
  GCTGAGGACTGCTCTGCTGTATAACCACAGCATACTCTCCGACAAGGCAAAGGCATTCGACGGCACCAGC
  CTTTTCCTCTCAGAAAAGCTGGACCACAAAGTCACAGAACTGACAAGTGAAACCCAAAGAGGGGAGACTG
  TGAAGATAACTCTCACCCTGACCAGTGAGCTGTTCCCAAACTCCCCGATGTGCATTCAGTTTTTCAATGT
  CATCTTCAGAAAGATCTTTAAAAAGTTGAGCATGTACCAGATTGGACGGAACTACTATAAGCCTTCAGAG
  CCAGTGGAGATCCCCCAGTACAAATTGTCCCTGTGGCCTGGGTTTGCCATTTCTGTGTCTCGCTTTGAAA
  GCAAACTCCTGTTCAATGCTGACGTAAACTATAAAGTCCTCCGCAATGAGACTGTTCTGGAATTCATGAC
  TGATCTCTGCCTCAGAACCGACATGTCATGCTTCACTGAGACGTGCCACAAACAGCTAGTGGGGCTCATT
  GTCCTTACCAGGTACAACAACAAAACATATCGTATCGATGACATTGACTGGTCTGTGAAGCCCACACATG
  CCTTTCAGAAGCGGGATGGCTCAGAGACCACGTATGTGGATTACTACAAGCAGCAATATGATATCACCTT
  ATCTGACCTAAATCAGCCAGTGCTTGTCAGTCTGTTGAAAAGAAGGAGAAATGATAACTCCGAGCCTCAG
  ATGGTCCACCTGATCCCGGAGCTCTGCTTTCTCACGGGCCTGACCAGCCAAGCAACCTCAGACTTCCGCT
  TGATGAAGGCAGTCGCTGAAGAAACTCGGCTCAGTCCTATGGGAAGGCAGCAGCAACTAGCCCGACTGGC
  AGATGACATCCAGAGAAACCAAGTGACTCGGTTTGAGCTTGAGACCTGGGGATTGCATTTTGGAAGCCAG
  CTATCCCTGACCGGCCGGGTTGTTCCCTCTGAAAAGATCCTGCTGCAGGACCACACATGTCAACCTGCAT
  TTGCTGCTGATTGGTCCAAAGATATGCGATCTTGCAAGGTTCTGAGTTCTGAGCCTTTGAATACATGGTT
  GATCGTGTGCTGTAGCAGGGCTGAGCATTTGATTGAATCCTTCCTGAGCTGTCTGAGGAGAGTTGGAGGT
  TCCACGGGATTCAGTGTGGGCCACCCAAAAATCATAAAAGTGGATGAAAACCCTGCAGCGTTCCTTCGAG
  CCATCCAGCTGCACGTCAACCCCGATGTTCAGTTGGTGATGTGCATCCTGCCTTCTAATCAGAAGAACTA
  TTACGACTCCATTAAAAGATATTTGAGCTCCGACTGCCCGGTCCCAAGCCAGTGTGTGCTGACCCGGACC
  TTGAACAAACAGGGAATGATGCTGAGTGTGGCCACCAAGATCGCCATGCAAATGACCTGCAAACTCGGGG
  GCGAGCTGTGGGCTGTGGAGATCCCATTGAAGTCACTGATGGTCGTGGGCATCGACATCTGTAGAGACGC
  CCTCAGCAAGGATGTAGCGGTCGTCGGGTTTGTCGCCAGCATTAACTCCAGGATCACCAGGTGGTTTTCC
  CGCTGTGTCCTCCAGAGAACGGCAGCTGATATTGCAGATTGCCTGAAAGTCTGCATGACTGGTGCTCTCA
  ACAAGTGGTACAAACACAACCACGATTTGCCGGCACGGATAATCGCGTACCGGGATGGTGTGGGCAACGG
  CCAGCTAAAGGCAGTTTTGGAATACGAAGTCCCACAGCTGCTGAGCAGTGTGACAGAATGTGGCTCCGAC
  GCCAGGAGCTGTAGACTGTCAGTGGTCGTGGTCAGGAAGAGGTGTCTGCTGCGTCTCTTTGCCGAGTCTG
  GCCATACGCTACAGAACCCCCCACTTGGCACTGTCGTGGACTCAGAAGCAACACGTCCAGAGTGGTATGA
  CTTCTACCTGACCAGCCAGACTGCTAACCGGGGGACTGTTAGTCCCACCTACTACAATGTCATCTATGAC
  GACAACGCTCTGAAGCCTGACCATATGCAGCGGCTGACCTTCAAACTGTGCCACCTCTACTACAACTGGC
  AGGGCGTAATCAGTGTCCCTGCTCCGTGCCAGTATGCACACAAGCTGACCTTCCTGGTGGCACAAAGCAT
  CCACAAGGAACCAAGTCTGGAATTAGCCAATAAGCTCTTCTACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217245 representing NM_001271133
  Red=Cloning site Green=Tags(s)
  
  MSGRARVRARGITTGHSAREVERAPRDLMVSASVYELGVSSGDGRRTLVEKRGNGRQDFVDLGVCTREKL
  THVKDSKTGSSGIPVQLATNLFHLDLPQDWQLYQYHVTYSPDLASRRLRTALLYNHSILSDKAKAFDGTS
  LFLSEKLDHKVTELTSETQRGETVKITLTLTSELFPNSPMCIQFFNVIFRKIFKKLSMYQIGRNYYKPSE
  PVEIPQYKLSLWPGFAISVSRFESKLLFNADVNYKVLRNETVLEFMTDLCLRTDMSCFTETCHKQLVGLI
  VLTRYNNKTYRIDDIDWSVKPTHAFQKRDGSETTYVDYYKQQYDITLSDLNQPVLVSLLKRRRNDNSEPQ
  MVHLIPELCFLTGLTSQATSDFRLMKAVAEETRLSPMGRQQQLARLADDIQRNQVTRFELETWGLHFGSQ
  LSLTGRVVPSEKILLQDHTCQPAFAADWSKDMRSCKVLSSEPLNTWLIVCCSRAEHLIESFLSCLRRVGG
  STGFSVGHPKIIKVDENPAAFLRAIQLHVNPDVQLVMCILPSNQKNYYDSIKRYLSSDCPVPSQCVLTRT
  LNKQGMMLSVATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDICRDALSKDVAVVGFVASINSRITRWFS
  RCVLQRTAADIADCLKVCMTGALNKWYKHNHDLPARIIAYRDGVGNGQLKAVLEYEVPQLLSSVTECGSD
  ARSCRLSVVVVRKRCLLRLFAESGHTLQNPPLGTVVDSEATRPEWYDFYLTSQTANRGTVSPTYYNVIYD
  DNALKPDHMQRLTFKLCHLYYNWQGVISVPAPCQYAHKLTFLVAQSIHKEPSLELANKLFYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271133
• ORF Size: 2496 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271133.1, NP_001258062.1
• RefSeq Size: 3109
• RefSeq ORF: 2499
• Locus ID: 689972
• MW: 94.4 kDa