Atp4a (NM_012509) Rat Tagged ORF Clone

CAT#: RR217427

Atp4a (myc-DDK-tagged) - Rat ATPase, H+/K+ exchanging, alpha polypeptide (Atp4a)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp4a
• Synonyms: Hka; Hkatpc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217427 representing NM_012509
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGGCAGAGAATTATGAATTGTACTCAGTGGAACTGGGGACTGGCCCTGGTGGGAACATGGCTG
  CCAAGATGAGCAAGAAGAAGGCGGGAGGCGGGGGAGGCAAGAAGAAGGAGAAGCTAGAGAACATGAAGAA
  GGAGATGGAGATGAACGACCACCAGTTGTCAGTGTCTGAGCTGGAGCAGAAGTACCAGACCAGTGCCACC
  AAGGGCCTGAAGGCGAGTCTGGCAGCTGAGCTGCTGCTGAGGGATGGGCCCAATGCACTCCGGCCACCTC
  GGGGCACCCCTGAGTACGTGAAGTTCGCCCGGCAGCTGGCAGGCGGTCTGCAGTGCCTCATGTGGGTGGC
  TGCAGCCATCTGCCTCATTGCCTTTGCGATTCAGGCCAGCGAGGGAGACCTGACCACTGATGACAATTTG
  TACCTGGCGTTGGCACTCATTGCTGTGGTTGTGGTCACTGGCTGTTTTGGCTACTACCAGGAGTTCAAGA
  GCACAAATATCATCGCCAGCTTCAAGAATCTTGTACCCCAGCAAGCCACAGTGATCCGAGACGGGGATAA
  GTTCCAGATCAACGCGGATCAGCTTGTGGTGGGCGACCTGGTAGAGATGAAAGGCGGGGACCGCGTCCCA
  GCAGACATCCGAATTCTGTCAGCCCAGGGCTGCAAGGTGGACAACTCCTCGCTTACTGGAGAGTCTGAAC
  CGCAGACCCGCTCACCTGAGTGTACACACGAGAGTCCCCTTGAGACCCGCAACATCGCCTTCTTCTCCAC
  CATGTGTCTGGAGGGCACGGCGCAGGGCTTGGTGGTGAGCACCGGTGATCGCACCATCATTGGACGCATC
  GCCTCTCTGGCTTCGGGTGTGGAAAACGAGAAGACTCCTATCGCGATCGAGATCGAACATTTTGTGGACA
  TCATTGCTGGCCTGGCCATCCTCTTCGGTGCCACATTCTTTGTGGTGGCCATGTGTATCGGCTATACCTT
  CCTTCGGGCCATGGTCTTCTTCATGGCCATTGTGGTAGCCTATGTGCCTGAGGGGCTGCTGGCTACTGTC
  ACGGTCTGCCTGTCACTGACAGCAAAGAGGCTGGCCAGTAAAAACTGTGTGGTCAAAAATCTGGAAGCAG
  TGGAGACCCTGGGTTCCACGTCAGTCATCTGCTCAGACAAGACAGGAACTCTTACTCAGAACCGCATGAC
  GGTGTCTCATCTATGGTTTGACAACCATATCCACACGGCGGACACCACAGAAGACCAGTCAGGGCAAACG
  TTCGACCAATCGTCGGAGACCTGGCGGGCGCTGTGCCGCGTGCTCACCCTGTGCAACCGCGCTGCCTTCA
  AGTCTGGCCAGGACGCCGTGCCAGTGCCCAAGCGCATCGTGATCGGAGACGCATCTGAGACTGCGCTGCT
  CAAGTTCTCGGAGTTGACGTTGGGCAACGCCATGGGTTATCGGGACCGCTTCCCCAAAGTTTGCGAGATC
  CCCTTCAACTCCACCAACAAGTTTCAGCTGTCCATTCACACTCTGGAGGATCCGCGCGACCCCCGGCACT
  TGCTGGTGATGAAGGGCGCCCCAGAGCGCGTGCTGGAGCGTTGCAGCTCCATCCTCATCAAGGGCCAGGA
  GCTGCCCCTGGACGAGCAATGGCGTGAGGCCTTCCAGACAGCCTACCTTAGCCTGGGAGGCCTAGGCGAA
  CGCGTTCTTGGTTTCTGCCAGCTCTACCTGAATGAGAAGGACTACCCGCCTGGCTACACCTTTGATGTGG
  AGGCCATGAACTTTCCAAGTAGTGGCCTCTGCTTTGCGGGACTTGTATCCATGATTGACCCTCCCCGGGC
  CACCGTTCCAGATGCTGTGCTCAAATGCCGTACGGCAGGCATCCGGGTGATCATGGTGACTGGTGACCAT
  CCCATCACAGCCAAGGCCATTGCAGCCAGTGTGGGGATCATCTCGGAAGGCAGCGAGACAGTGGAAGACA
  TCGCCGCCCGCCTCCGAATGCCTGTAGACCAGGTTAATAAGAAGGATGCCCGGGCCTGTGTGATCAATGG
  CATGCAGTTGAAGGACATGGACCCATCTGAGCTGGTGGAGGCGCTGCGCACCCACCCTGAGATGGTGTTT
  GCTCGAACCAGTCCTCAGCAGAAGCTGGTGATTGTGGAGAGCTGTCAGCGACTGGGTGCCATTGTGGCTG
  TAACAGGGGATGGTGTGAATGACTCCCCAGCCCTGAAGAAGGCTGACATTGGTGTAGCCATGGGCATTGC
  TGGCTCCGATGCTGCTAAAAATGCTGCTGACATGATCTTGCTGGATGACAACTTCGCTTCCATTGTGACG
  GGCGTGGAGCAGGGCCGACTGATCTTTGACAACCTGAAGAAATCCATCGCCTATACACTGACCAAGAACA
  TTCCAGAACTGACACCCTACCTTATCTATATCACTGTCAGTGTGCCCCTACCCCTTGGGTGTATCACCAT
  TCTCTTCATAGAACTCTGCACAGATATCTTTCCATCTGTGTCCCTGGCATATGAAAAGGCCGAGAGTGAC
  ATCATGCACCTGCGCCCACGGAACCCCAGGCGGGACCGGTTGGTCAATGAACCCCTGGCTGCTTATTCCT
  ATTTTCAGATTGGTGCCATTCAGTCATTTGCCGGCTTTGCTGACTACTTCACGGCCATGGCCCAGGAGGG
  CTGGTTCCCTCTGCTGTGTGTGGGGCTGCGACCACAATGGGAGGACCACCATCTACAAGATCTTCAAGAC
  AGCTACGGCCAGGAATGGACATTTGGTCAGCGTCTGTACCAGCAGTACACCTGTTACACCGTGTTCTTCA
  TCAGCATCGAGATGTGCCAGATCGCTGATGTCCTCATCCGCAAGACACGCCGCCTCTCCGCTTTCCAGCA
  GGGATTCTTCAGGAACAGGATCCTGGTGATCGCCATCGTGTTTCAGGTCTGCATTGGCTGCTTCCTGTGC
  TACTGCCCAGGGATGCCCAACATCTTCAACTTCATGCCCATCCGGTTCCAGTGGTGGCTGGTCCCCATGC
  CCTTTGGCCTTCTCATCTTTGTCTATGATGAGATCCGGAAACTTGGAGTTCGCTGTTGCCCAGGGAGCTG
  GTGGGACCAGGAACTCTACTAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217427 representing NM_012509
  Red=Cloning site Green=Tags(s)
  
  MGKAENYELYSVELGTGPGGNMAAKMSKKKAGGGGGKKKEKLENMKKEMEMNDHQLSVSELEQKYQTSAT
  KGLKASLAAELLLRDGPNALRPPRGTPEYVKFARQLAGGLQCLMWVAAAICLIAFAIQASEGDLTTDDNL
  YLALALIAVVVVTGCFGYYQEFKSTNIIASFKNLVPQQATVIRDGDKFQINADQLVVGDLVEMKGGDRVP
  ADIRILSAQGCKVDNSSLTGESEPQTRSPECTHESPLETRNIAFFSTMCLEGTAQGLVVSTGDRTIIGRI
  ASLASGVENEKTPIAIEIEHFVDIIAGLAILFGATFFVVAMCIGYTFLRAMVFFMAIVVAYVPEGLLATV
  TVCLSLTAKRLASKNCVVKNLEAVETLGSTSVICSDKTGTLTQNRMTVSHLWFDNHIHTADTTEDQSGQT
  FDQSSETWRALCRVLTLCNRAAFKSGQDAVPVPKRIVIGDASETALLKFSELTLGNAMGYRDRFPKVCEI
  PFNSTNKFQLSIHTLEDPRDPRHLLVMKGAPERVLERCSSILIKGQELPLDEQWREAFQTAYLSLGGLGE
  RVLGFCQLYLNEKDYPPGYTFDVEAMNFPSSGLCFAGLVSMIDPPRATVPDAVLKCRTAGIRVIMVTGDH
  PITAKAIAASVGIISEGSETVEDIAARLRMPVDQVNKKDARACVINGMQLKDMDPSELVEALRTHPEMVF
  ARTSPQQKLVIVESCQRLGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDAAKNAADMILLDDNFASIVT
  GVEQGRLIFDNLKKSIAYTLTKNIPELTPYLIYITVSVPLPLGCITILFIELCTDIFPSVSLAYEKAESD
  IMHLRPRNPRRDRLVNEPLAAYSYFQIGAIQSFAGFADYFTAMAQEGWFPLLCVGLRPQWEDHHLQDLQD
  SYGQEWTFGQRLYQQYTCYTVFFISIEMCQIADVLIRKTRRLSAFQQGFFRNRILVIAIVFQVCIGCFLC
  YCPGMPNIFNFMPIRFQWWLVPMPFGLLIFVYDEIRKLGVRCCPGSWWDQELYY
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012509
• ORF Size: 3102 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012509.1, NP_036641.1
• RefSeq Size: 3105
• RefSeq ORF: 3105
• Locus ID: 24216
• MW: 114.1 kDa
• Gene Summary: catalyzes H+ and K+ exchange; may play a role in secretion of stomach acids [RGD, Feb 2006]