Srebf1 (NM_001276708) Rat Tagged ORF Clone

CAT#: RR217481

Srebf1 (myc-DDK-tagged) - Rat sterol regulatory element binding transcription factor 1 (Srebf1), transcript variant 2

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srebf1
• Synonyms: ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217481 representing NM_001276708
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGCACATTTGAAGACATGCTTCAGCTCATCAACAACCAAGACAGTGACTTCCCTGGCCTATTTG
  ATGCCCCCTATGCTGGGGGTGAGACAGGAGACACAGGCCCCAGCAGCCCTGGTGCCAGCTCTCCTGAGAG
  CTTCTCTTCTCCTGCTTCTCTGGGCTCCTCTCTGGAAGCCTTCCTGGGAGGACCCAAGGTGACACCTGCA
  CCCTTGTCCCCTCCACCATCGGCACCCACTGCTGTAAAGATGTACCCGTCCGTGCCCCCCTTCTCCCCTG
  GGCCTGGAATCAAAGAGGAGCCAGTGCCACTCACCATCCTGCAGCCCCCAGCACCACAGCCATCGCCAGG
  GACCCTGTTGCCTCCGAGCTTCCCTCCTCCACCTGTGCAGCTCAGCCCTGCTCCTGTGCTGGGGTACTCA
  AGCCTGCCTTCCGGCTTCTCAGGAACCCTTCCTGGGAACACCCAGCAGACGCCATCTAGCCTGCCACTGG
  GCTCCACGCCAGGAATCTCGCCCACCCCCTTACACACCCAGGTCCAGAGCTCGGCCGCCCAGCAGCCGCC
  GCCAGCCTCAGCAGCCCCTAGAATGAGCACTGTGGCCTCACAGATCCAGCAGGTCCCCGTTGTACTGCAG
  CCACACTTCATCAAGGCAGACTCGCTGCTGCTGACAGCTGTAAAGACAGACACAGGAGCCACAATGAAGA
  CCGCAGGCATCAACACCCTGGCTCCTGGCACAGCCGTGCAGGCAGGCCCCTTGCAGACCCTGGTGAGTGG
  AGGGACCATCCTGGCCACAGTACCACTGGTTGTGGACACAGACAAACTGCCCATCCACCGACTAGCAGCT
  GGTGGCAAGGCCCTGGGCTCAGCTCAGAGCCGTGGTGAGAAGCGCACAGCCCACAATGCCATTGAGAAGC
  GCTACCGTTCCTCTATCAATGACAAGATTGTGGAGCTCAAGGACCTGGTGGTGGGCACTGAGGCAAAGCT
  GAATAAATCTGCTGTCTTGCGCAAGGCCATCGACTACATCCGCTTCTTACAGCACAGCAACCAGAAACTC
  AAGCAGGAGAACCTGACCCTGCGAAGTGCTCACAAAAGCAAATCACTGAAAGACCTGGTGTCAGCTTGTG
  GCAGTGGAGGAGGCACAGATGTGTCTATGGAGGGCATGAAACCTGAAGTGGTAGAAACGCTGACCCCTCC
  ACCCTCAGACGCCGGCTCACCCTCCCAGAGTAGCCCCTTGTCCTTGGGCAGCAGAGGCAGCAGCAGTGGT
  GGCAGTGACTCTGAGCCCGACAGCCCAGCCTTTGAGGATAACCAGGTGAAAGCCCAGCGGCTGCCTTCAC
  ATAGCCGAGGCATGCTGGACCGCTCCCGCCTGGCCCTGTGTGTACTGGTCTTCCTGTGTCTGACCTGCAA
  CCCATTGGCCTCACTGTTTGGCTGGGGCATCCTCACTCCCTCTGATGCTTCGGGTGTGCACCGTAGTTCT
  GGGCGCAGCATGCTGGAGGCCGAGAGCAGAGATGGCTCTAATTGGACCCAGTGGTTGCTGCCACCCCTAG
  TCTGGCTGGCCAATGGACTACTAGTGTTGGCCTGCTTGGCTCTTCTCTTTGTCTACGGGGAACCTGTGAC
  CAGGCCACACTCCGGCCCGGCTGTACACTTCTGGAGACATCGCAAACAAGCTGACCTGGATTTGGCCCGG
  GGAGATTTTGCCCAGGCCGCTCAACAGCTGTGGCTGGCCTTGCAAGCCCTGGGCCGGCCCCTGCCCACCT
  CAAACCTGGATCTGGCCTGCAGCCTGCTTTGGAACCTCGTCCGCCACCTGCTGCAGCGTCTTTGGGTGGG
  CCGCTGGCTGGCAGGCCAGGCTGGGGGCCTGCAGAGGGACTACAGGCTGAGAAAGGATGCTCGTGCCAGT
  GCCCGAGATGCGGCTGTCGTCTACCATAAGCTGCACCAGCTGCATGCCATGGGCAAGTACACAGGAGGCC
  ATCTTGTTGCTTCTAACCTGGCACTGAGTGCCCTTAACCTGGCTGAGTGTGCAGGAGATGCTATATCCAT
  GGCAACACTGGCAGAGATCTACGTGGCAGCTGCCCTAAGGGTCAAAACCAGCCTCCCCAGAGCCTTGCAC
  TTCTTGACACGTTTCTTCCTGAGTAGTGCCCGCCAGGCCTGCCTGGCACAGAGTGGTGCAGTGCCTCTTG
  CCATGCAGTGGCTCTGCCACCCTGTAGGTCACCGTTTCTTCGTGGATGGGGACTGGGCTGTACACGGTGC
  CCCCCAGGAGAGTCTGTACAGCGTGGCTGGGAACCCAGTGGATCCACTGGCCCAGGTGACCCGACTATTC
  TGTGAACATCTCCTGGAGCGAGCATTGAACTGTATCGCTCAGCCCAGCCCAGGGGCAGCTGATGGAGACA
  GGGAGTTCTCAGATGCTCTTGGATATCTACAGTTGCTAAATAGCTGTTCTGACGCTGTCGGAGCTCCTGC
  GTGCAGCTTCTCTGTCAGTTCCAGCATGGCTACCACCACTGGCACAGACCCAGTGGCCAAGTGGTGGGCC
  TCACTGACAGCCGTGGTGATCCACTGGCTGAGGCGGGATGAGGAGGCAGCTGAACGCTTATACCCACTGG
  TAGAGCACATTCCCCAAGTGCTGCAGGAAACTGAGAGACCCCTTCCCAGGGCAGCTCTGTACTCCTTCAA
  GGCTGCCCGGGCTCTGCTGGACCACAGAAAGGTGGAATCCAGCCCAGCCAGCCTGGCCATCTGTGAGAAG
  GCCAGTGGGTACCTGCGGGACAGCTTAGCCTCTACATCAACTGCCAGTTCCATTGACAAGGCCATGCAGC
  TGCTCCTGTGTGATCTACTTCTTGTGGCCCGCACCAGCCTATGGCGGCGCCAACAGTCAGCAGCTTCAGC
  CCAGGGAGCTCACGGTACCAGCAATGGACCCCAGGCCTCTGCTCTGGAGCTGCGTGGTTTCCAACATGAC
  CTGAGCAGCCTGAGGCGCTTGGCACAGAGCTTCCGGCCTGCTATGAGGAGGGTCTTCCTACATGAGGCCA
  CAGCTCGGCTGATGGCAGGAGCAAGTCCTGCCCGGACACACCAGCTCCTGGACCGCAGTCTGCGGAGGCG
  GGCAGGTTCCAGTGGCAAAGGAGGCGCTGCAGCTGAGCTGGAGCCTCGACCCACATGGCGGGAGCACACA
  GAGGCCTTGCTGTTGGCCTCCTGCTATCTGCCCCCTGCCTTCCTGTCGGCCCCCGGGCAGCGAGTGAGCA
  TGCTGGCTGAGGCAGCGCGCACCGTGGAGAAGCTTGGCGATCACCGGCTCCTGCTTGACTGCCAGCAGAT
  GCTCCTGCGCCTGGGTGGCGGGACCACTGTCACTTCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217481 representing NM_001276708
  Red=Cloning site Green=Tags(s)
  
  MDCTFEDMLQLINNQDSDFPGLFDAPYAGGETGDTGPSSPGASSPESFSSPASLGSSLEAFLGGPKVTPA
  PLSPPPSAPTAVKMYPSVPPFSPGPGIKEEPVPLTILQPPAPQPSPGTLLPPSFPPPPVQLSPAPVLGYS
  SLPSGFSGTLPGNTQQTPSSLPLGSTPGISPTPLHTQVQSSAAQQPPPASAAPRMSTVASQIQQVPVVLQ
  PHFIKADSLLLTAVKTDTGATMKTAGINTLAPGTAVQAGPLQTLVSGGTILATVPLVVDTDKLPIHRLAA
  GGKALGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKL
  KQENLTLRSAHKSKSLKDLVSACGSGGGTDVSMEGMKPEVVETLTPPPSDAGSPSQSSPLSLGSRGSSSG
  GSDSEPDSPAFEDNQVKAQRLPSHSRGMLDRSRLALCVLVFLCLTCNPLASLFGWGILTPSDASGVHRSS
  GRSMLEAESRDGSNWTQWLLPPLVWLANGLLVLACLALLFVYGEPVTRPHSGPAVHFWRHRKQADLDLAR
  GDFAQAAQQLWLALQALGRPLPTSNLDLACSLLWNLVRHLLQRLWVGRWLAGQAGGLQRDYRLRKDARAS
  ARDAAVVYHKLHQLHAMGKYTGGHLVASNLALSALNLAECAGDAISMATLAEIYVAAALRVKTSLPRALH
  FLTRFFLSSARQACLAQSGAVPLAMQWLCHPVGHRFFVDGDWAVHGAPQESLYSVAGNPVDPLAQVTRLF
  CEHLLERALNCIAQPSPGAADGDREFSDALGYLQLLNSCSDAVGAPACSFSVSSSMATTTGTDPVAKWWA
  SLTAVVIHWLRRDEEAAERLYPLVEHIPQVLQETERPLPRAALYSFKAARALLDHRKVESSPASLAICEK
  ASGYLRDSLASTSTASSIDKAMQLLLCDLLLVARTSLWRRQQSAASAQGAHGTSNGPQASALELRGFQHD
  LSSLRRLAQSFRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRRRAGSSGKGGAAAELEPRPTWREHT
  EALLLASCYLPPAFLSAPGQRVSMLAEAARTVEKLGDHRLLLDCQQMLLRLGGGTTVTSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276708
• ORF Size: 3330 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001276708.1, NP_001263637.1
• RefSeq Size: 4264
• RefSeq ORF: 3333
• Locus ID: 78968
• MW: 118 kDa
• Gene Summary: transcription factor; binds to the sterol regulatory element 1; regulates the transcription of genes important for sterol biosynthesis [RGD, Feb 2006]