Nfasc (NM_001160313) Rat Tagged ORF Clone

CAT#: RR217536

Nfasc (myc-DDK-tagged) - Rat neurofascin (Nfasc), transcript variant 2

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfasc
• Synonyms: NF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217536 representing NM_001160313
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT
  GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT
  CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG
  TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA
  ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC
  CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT
  CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA
  CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC
  TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCAACAACCCCTATA
  ATGACTCGTCTTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCGGAAAGAACACCTAGCTT
  CATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATT
  GCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCA
  AGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG
  CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGG
  CTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGA
  ACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGGCACCACCCAACCCCAACCG
  TGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAAC
  ACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGC
  TGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGG
  GTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCAC
  GTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG
  CCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG
  GATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCC
  TCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATTGGAAACAGGATGAAAAAGG
  AAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCAC
  CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGCTGATCAGGCCACTCCGACTAACCGT
  TTGGCTGCCCTGCCCAAAGGGCGGCCAGACCGACCCAGGGACCTGGAGCTCACTGACCTGGCGGAAAGGA
  GTGTGAGGCTGACCTGGATCCCGGGGGATGATAACAACAGTCCCATCACAGACTACGTTGTCCAGTTTGA
  AGAGGACCAGTTCCAGCCAGGAGTCTGGCATGACCACTCCAAGTTCCCAGGCAGTGTCAACTCAGCCGTC
  CTCCATCTGTCCCCGTATGTCAACTATCAGTTCCGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACC
  CCAGCCTTCCATCCGAGCGGTACCGAACAAGCGGGGCACCCCCTGAATCCAACCCCAGTGATGTGAAGGG
  CGAAGGGACACGAAAAAACAATATGGAGATCACATGGACGCCTATGAATGCTACCTCCGCCTTTGGCCCC
  AACCTGCGCTACATTGTCAAGTGGCGACGGAGAGAAACCCGAGAGACTTGGAACAATGTCACCGTGTGGG
  GCTCTCGCTATGTGGTGGGGCAGACCCCTGTCTACGTACCCTATGAGATCCGAGTCCAGGCTGAAAATGA
  CTTTGGGAAAGGCCCGGAGCCTGAAACCGTCATTGGGTACTCGGGGGAAGATTATCCCAGGGCTGCACCC
  ACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTTCAGTGGAACCGCGTCTACCCTGACA
  CGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGGAAAGCAGTTTGCTGAAGAACCTGTG
  GGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGCGACCGCCCCCGGGGCGTGGTGGGTCGCCTGTTC
  CCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGAGGTGATGGGCCTCGAAGTGAAACCA
  AGGAATTCACCACCCCGGAAGGAGTACCCAGTGCCCCCAGGCGTTTCAGAGTCCGACAGCCCAACCTGGA
  GACCATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGATCCTGATTGGATACACGCTCAGATAC
  GTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCT
  CGGTGCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCAGTGCCAGGACGCAGGTGGGCTCTGG
  AGAAGCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTACTCCAACTGCAGCTTACACCAACAAC
  CAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTTCTGGTGCTGATCC
  TGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACCCAGTGCGAGAAAAGAAGGATGTCCC
  CTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTACAGTGACGAGGACAACAAGCCCCTG
  CAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAGAGTGACGACAGCCTAGTGGACTACG
  GCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTGGCCAGTACACTGTCAGAAAGGACAA
  GGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGTCAATGCCATCTATTCCCTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217536 representing NM_001160313
  Red=Cloning site Green=Tags(s)
  
  MARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWT
  RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN
  LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR
  FHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECI
  ASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW
  LDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN
  TSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH
  VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDP
  SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNR
  LAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAV
  LHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGP
  NLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAP
  TEVKIRVLNSTAISLQWNRVYPDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLF
  PYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRY
  VPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNN
  QTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPL
  QGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160313
• ORF Size: 3567 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001160313.1, NP_001153785.1
• RefSeq Size: 9454
• RefSeq ORF: 3570
• Locus ID: 116690
• MW: 133.8 kDa
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]