Diaph1 (NM_001107393) Rat Tagged ORF Clone

CAT#: RR217569

Diaph1 (myc-DDK-tagged) - Rat diaphanous-related formin 1 (Diaph1)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Diaph1
• Synonyms: Diap1; DRF1; mDIA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217569 representing NM_001107393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGTCCGGCGGGGGCCTAGGGCCCGGCCGCGGGACCCGGGACAAGAAGAAGGGTCGGAGCCCGG
  ATGAGCTGCCTGCGACGGGCGGCGACGGCGGCAAACATAAGAAATTTACTTTGAAGCGGCTCATGGCAGA
  CGAGCTGGAGAGATTTACCAGCATGAGGATTAAGAAGGAGAAGGAAAAGCCCAATTCTGCTCATAGAAAC
  TCCTCTGCATCGTATGGAGATGACCCCACTGCTCAGTCACTGCAGGACATCTCAGATGATCAAGTTCTTG
  TCCTCTTTGAGCAGATGCTGGTGGATATGAACCTGAATGAGGAGAAACAGCAGCCTTTGCGAGAGAAGGA
  CATTGTCATCAAGAGGGAGATGGTGTCCCAGTATCTGCATACTTCCAAAGCTGGCATGAACCAGAAGGAG
  AGTTCCAGGTCTGCCATGATGTACATTCAGGAGCTGAGGTCAGGCTTGCGGGATATGCACCTGCTTAGCT
  GCCTGGAGTCCCTTCGAGTCTCTCTCAACAATAACCCTGTCAGTTGGGTGCAGACATTTGGTGCTGAGGG
  CCTAGCCTCCTTATTGGACATCCTCAAACGACTCCATGATGAGAAAGAGGAGACTTCTGGAAACTACGAC
  AGCCGGAACCAGCATGAGATTATCCGCTGTTTGAAAGCTTTCATGAACAACAAGTTTGGAATCAAAACCA
  TGTTGGAGACGGAAGAAGGAATCCTACTGCTGGTCAGAGCCATGGATCCTGCTGTTCCCAATATGATGAT
  TGATGCAGCAAAGCTGCTGTCTGCCCTCTGTATCCTACCGCAGCCAGAGGACATGAATGAACGAGTTTTA
  GAGGCTATGACAGAGAGAGCTGAAATGGAAGAGGTTGAACGCTTCCAGCCACTGCTGGATGGGTTAAAAA
  GTGGGACGTCTATTGCACTCAAAGTGGGATGCCTACAGCTGATCAATGCTCTCATCACTCCCGCTGAGGA
  ACTTGACTTCCGGGTTCACATCCGAAGTGAGCTGATGCGCTTGGGACTGCATCAAGTGTTGCAGGAGCTT
  CGAGAGATTGACAATGATGATATGCGAGTACAGCTCAATGTGTTTGATGAACAAGGGGATGAAGATTTCT
  TCGATCTGAAGGGACGACTGGATGATATCCGCATGGAGATGGATGACTTTGGTGAAGTTTTCCAGATTAT
  TTTAAACACAGTGAAAGATTCCAAGGCAGAACCACACTTCCTGTCTATCTTGCAGCATCTCCTGTTGGTC
  CGAAATGATTATGAAGCTAGGCCGCAGTACTATAAATTGATTGAAGAATGTGTTTCTCAGATAGTTCTAC
  ACAAAAATGGAACTGATCCTGACTTCAAGTGCCGGCACCTGCAGATTGATATTGAGGGGTTGGTTGATCA
  AATGATTGATAAAACGAAGGTGGAAAAATCTGAGGCCAAAGCTACAGAGCTGGAAAAAAAGTTGGACTCA
  GAATTAACAGCCCGGCATGAGTTACAAGTAGAAATGAAAAAGATGGAAAATGACTTTGAGCAGAAACTTC
  AGGATCTTCAAGGAGAAAAGGATGCCTTGGATTCTGAAAAGCAGCAGATCACTACACAAAAACAAGACCT
  GGAAGCAGAGGTGTCCAAGCTGACAGGAGAGGTTGCCAAGCTGTCAAAAGAACTAGAAGATGCCAAGAAG
  GAAATGGCTTCTCTCTCTGCTGTGGCTGTTGCACCTTCAGTTTCTAGTAGTGCTGCTGTTCCCCAAGCCC
  CTCCTCTGCCTGGCACTTCAGGCACTGTTATTCCACCTCCCCCACCACCCCCTCTCCCTGGAGGTGCCGT
  CCCACCCCCTCCTCCTCCTCCTCTGCCTGCAGGTACTGGTATCCCTCCACCTCCTCCTTTACCTGGAGGT
  GCTTGTATATCCTCTTCCCCACAGTTGTTTGGCAGTACTGCCATTCCCCCACCTCCTCCTTTGCCTGGAG
  CTACTGCCATCCCCCCACCTCCCCCTTTGCCTGGAGATACTGCCATCCCCCCACCTCCCCCTTTGCCTGG
  AGNTACTGCCATCCCCCCTCCTCCTCCTTTGCCTGGAGCTACTGGTGTACCACCACCTCCTCCCCCTTTA
  CCTGGAAGTGTTGGTGTTCCCCCACCCCCTCCCTTGCCTGGAGGACCAGGACTGCCTCCTCCTCCTCCCC
  CCTTTCCTGGAGCACCTGGCATTCCCCCCCCACCGCCTGGTATGGGCGTGCCTCCACCTCCCCCCTTTGG
  ATTTGGGATTCCTGCAGCCCCAGTTCTGCCATTTGGATTAACCCCCAAAAAAGTTTATAAGCCAGAGGTG
  CAACTCCGGAGGCCAAACTGGTCCAAGTTCGTGGCTGAGGACCTTTCCCAGGACTGCTTCTGGACAAAGG
  TGAAGGAGGACCGCTTTGAGAACAATGAACTATTTGCCAAACTTACCCTTGCCTTCTCTGCCCAGACCAA
  GACTTCTAAAGCCAAGAAGGATCAAGAAGGTGGAGAAGAAAAGAAATCTGTTCAAAAGAAGAAAGTAAAA
  GAGCTGAAAGTGCTGGATTCAAAGACAGCCCAGAATCTCTCAATCTTTTTGGGTTCATTCCGTATGCCCT
  ATCAAGAGATTAAGAACGTTATCCTGGAGGTGAATGAGGCTGTTCTCACGGAGTCTATGATCCAGAACCT
  CATTAAGCAGATGCCAGAACCAGAGCAGTTAAAAATGCTTTCTGAACTGAAGGAGGAGTACGATGATCTG
  GCTGAGTCAGAGCAGTTCGGCGTGGTGATGGGCACGGTTCCCCGTCTTCGGCCTCGCCTCAATGCCATCC
  TTTTCAAGCTACAGTTCAGTGAGCAAGTTGAGAATATCAAGCCAGAGATTGTATCTGTCACTGCGGCATG
  TGAGGAGCTGCGTAAGAGTGAGAACTTCTCTAGCCTCCTGGAGCTCACACTGCTCGTTGGAAACTATATG
  AATGCGGGCTCCAGGAATGCTGGAGCTTTTGGCTTCAATATCAGCTTCCTTTGTAAGCTTCGAGACACCA
  AGTCTGCAGATCAGAAGATGACTCTGTTGCATTTCTTGGCTGAGTTATGTGAGACTGACCACCCCGATGT
  CCTCAAGTTTCCTGATGAGCTTGCCCATGTAGAGAAAGCCAGCAGAGTTTCTGCTGAGAACTTGCAGAAG
  AATTTAGATCAGATGAAGAAACAGATTGCTGATGTGGAACGCGATGTTCAAAATTTCCCAGCTGCCACAG
  ACGAAAAAGACAAGTTTGTTGAGAAGATGACTAGCTTTGTGAAGGATGCACAGGAACAGTATAACAAGCT
  ACGGATGATGCACTCCAACATGGAGACTCTCTATAAGGAGCTAGGCGACTACTTCGTCTTTGACCCTAAG
  AAGTTGTCTGTAGAAGAATTTTTTATGGATCTGCACAACTTTAGGAATATGTTTTTGCAAGCAGTCAAGG
  AGAACCAGAAGCGCCGGGAAACAGAAGAAAAGATGAGGAGAGCAAAACTAGCCAAGGAAAAGGCGGAAAA
  AGAGCGACTGGAGAAGCAGCAGAAGCGAGAGCAGCTCATAGACATGAATGCAGAGGGGGATGAGACAGGT
  GTGATGGACAGTCTTCTAGAAGCTCTGCAGTCAGGAGCAGCATTCCGACGGAAGAGAGGGCCCCGGCAGG
  TCAACAGGAAAGCTGGGTGTGCAGTCACATCTCTGCTAGCCTCGGAGCTGACCAAGGATGATGCCGTGGC
  TGCTAGTTCTGCTAAGGTGCCCAAGAAAAGTGAAGGAGTCACCACAATCCTTGAAGAAGCCAAGGAACTG
  GTTGGCCGTGCAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217569 representing NM_001107393
  Red=Cloning site Green=Tags(s)
  
  MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRN
  SSASYGDDPTAQSLQDISDDQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKE
  SSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYD
  SRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVL
  EAMTERAEMEEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQEL
  REIDNDDMRVQLNVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLV
  RNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIEGLVDQMIDKTKVEKSEAKATELEKKLDS
  ELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITTQKQDLEAEVSKLTGEVAKLSKELEDAKK
  EMASLSAVAVAPSVSSSAAVPQAPPLPGTSGTVIPPPPPPPLPGGAVPPPPPPPLPAGTGIPPPPPLPGG
  ACISSSPQLFGSTAIPPPPPLPGATAIPPPPPLPGDTAIPPPPPLPGXTAIPPPPPLPGATGVPPPPPPL
  PGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGIPAAPVLPFGLTPKKVYKPEV
  QLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVK
  ELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDL
  AESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYM
  NAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCETDHPDVLKFPDELAHVEKASRVSAENLQK
  NLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPK
  KLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETG
  VMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAVAASSAKVPKKSEGVTTILEEAKEL
  VGRAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107393
• ORF Size: 3795 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001107393.2, NP_001100863.2
• RefSeq Size: 5588
• RefSeq ORF: 3798
• Locus ID: 307483
• MW: 140.4 kDa