Map3k5 (NM_001277694) Rat Tagged ORF Clone

CAT#: RR217609

Map3k5 (myc-DDK-tagged) - Rat mitogen-activated protein kinase kinase kinase 5 (Map3k5)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map3k5
• Synonyms: Ask1; RGD1306565
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217609 representing NM_001277694
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCGAAGCCGGCGAGGGCATCACTTTCTCCGTGCCGCCCATTTTGTCCGCGGGTTTCTGCCCCA
  TCCCGGAGGGCGGCAGCTGCCGGAGGGGAGGAGGAGCGGCGACGGCGGCGGAGGGCGAGCCGAGCCTGCA
  GCCCTTGCTTATGCCGCCGCCGCCACCACCGCCGGGCAGTTTCTGGAACGTGGAGAGCGCGGCGGCTCCT
  GGCACCAGCTGCCCTACCACCGCCCCCGGGAGCAGCGCCACCCGAGGCCGGGGCAACTCTGGCAGCGGGG
  GCGGCCGGCGTACCACCGTGGCGTATGTGATCAACGAGGCGAGCCAGGGGCAGCTGGTGGTGGCGGAGAG
  CGAGGCCCTGCAGAGCCTGCGGGAGGCGTGCGAGGCGGTGGGCGCCACCCTGGAGACCCTGCATTTCGGG
  AAGCTGGACTTTGGGGAAACCACCGTGCTGGACCGTTTTTACAACGCTGACATCGCCGTGGTAGAGATGA
  GTGACGCCTTCCGGCAGCCTTCCCTGTTTTATCATCTCGGAGTGAGAGAGAGCTTCAGCATGGCCAACAA
  CATCATCCTCTACTGCGATACTAATTCAGACTCGCTTCAGTCCCTGAAGGAAATCATTTGCCAGAAGAAC
  ACAGTGTGCACTGGGAACTACACCTTCATCCCCTACATGGTGACACCACACAACAAGGTCTACTGCTGTG
  ACAGCAGCTTCATGAAGGGCCTGACCGAGCTCATGCAGCCCAACTTCGAGCTCCTGCTGGGGCCGATCTG
  CTTACCTCTCGTGGACCGTTTCGTTCAGCTCCTGAAGGTGGCGCAAGCCAGTTCCAGCCAGTACTTCCGG
  GAATCTATACTCAACGACATCAGGAAAGCTCGGAATCTGTACACTGGTAAAGAGTTGGCGGCTGAGCTGG
  CGAGGATCCGGCAGCGGGTAGACAATATTGAAGTTCTGACGGCAGACATTGTTATCAACCTGTTGCTTTC
  CTACAGAGATATCCAGGACTATGACTCCATAGTGAAGCTGGTAGAGACCTTAGAAAAACTACCGACCTTC
  GATCTGGCCTCGCACCACCACGTGAAGTTTCATTATGCCTTTGCACTGAACAGGAGAAATCTCCCTGGAG
  ACAGAGCAAAAGCTCTTGACATCATGATCCCCATGGTGCAAAGTGAAGAGCAAGTTGCTTCGGATATGTA
  CTGTCTCGTTGGCCGAATCTACAAAGACATGTTTTTGGATTCAAATTTCACTGACACCGAAAGCAGGGAC
  CATGGAGCATCTTGGTTCAAAAAGGCGTTTGAGTCTGAGCCAACACTACAGTCAGGAATTAATTACGCAG
  TCCTCTTGCTGGCGGCTGGGCACCAGTTTGAATCTTCTTTTGAGCTTCGGAAAGTTGGAGTTAAACTGAG
  CAGCCTTCTGGGTAAAAAGGGGAACTTGGAGAAACTCCAGAGCTACTGGGAAGTCGGATTTTTTCTGGGA
  GCCAGCGTCCTGGCCAACGACCATCTGAGGGTCATTCAGGCATCTGAGAAACTGTTCAGACTGAAGACGC
  CAGCGTGGTACCTCAAGTCTATTGTGGAGACCATTTTGATATATAAGCATTTTGTGAAACTGACCACAGA
  GCAGCCCACAGCCAAGCAGGAGCTCGTGGACTTCTGGATGGATTTCCTGGTGGAGGCCACAAAGACAGAT
  GTTACTGTCGTTAGATTTCCAGTGTTAATATTAGAGCCAACGAAAATCTATCAGCCTTCTTACCTGTCGA
  TCAACAATGAAGTCGAGGAGAAGACGATCTCTATTTGGCATGTCCTCCCTGACGACAAGAAGGGTATCCA
  TGAGTGGAACTTTAACGCTTCCTCTGTCAGGGGGGTGAGTATCTCCAAGTTTGAAGAACGATGCTGTTTC
  CTTTATGTACTTCACAATTCCGACGACTTCCAAATCTACTTCTGCACAGAACTGCACTGTAAAAGGTTTT
  TCGAAATGGTGAACACCATCACCGAAGAGAAGGGGAGAGGCGCGGAGGAAGGAGACTGTGAAGGTGACTC
  CCTAGAGTATGACTACGAACACGATGAAAATGGAGACAGAGTTGTGCTAGGAAAGGGGACTTACGGGATA
  GTCTACGCAGGCCGAGACCTGAGCAACCAGGTCAGAATTGCTATTAAGGAAATCCCAGAGAGAGACAGCA
  GATACTCTCAGCCCCTGCATGAGGAAATTGCCTTACACAAGCATCTGAAGCACAAGAACATTGTCCAGTA
  TCTGGGCTCCTTCAGTGAGAACGGCTTCATCAAAATCTTCATGGAGCAAGTCCCAGGAGGAAGTCTCTCT
  GCTCTCCTCCGTTCAAAATGGGGCCCACTAAAAGACAATGAGCAGACGATTGGCTTCTATACGAAGCAAA
  TATTGGAAGGATTAAAATATCTTCATGACAATCAGATAGTCCACCGGGATATAAAGGGTGACAATGTATT
  GATTAATACCTACAGTGGCGTGCTCAAGATCTCTGACTTTGGGACATCCAAGAGGCTTGCTGGCATAAAC
  CCGTGCACCGAAACCTTTACAGGTACCCTTCAGTATATGGCGCCAGAAATCATCGATAAAGGACCACGAG
  GCTATGGAAAGGCCGCCGACATTTGGTCACTGGGCTGTACGATCATCGAAATGGCTACTGGAAAACCACC
  GTTTTATGAACTAGGAGAGCCCCAGGCAGCCATGTTCAAGGTGGGGATGTTTAAGGTCCACCCCGAGATC
  CCAGAGTCCATGTCTGCCGAGGCCAAGGCGTTCATACTGAAGTGTTTTGAACCAGACCCTGACAAGAGAG
  CCTGTGCTAATGACCTGCTTGTTGATGAGTTCCTAAAAGTTTCCAGCAAAAAGAAGAAGACACAGCCCAA
  ACTCTCGGGTATGTGGTTGCACGGAGAGGAGTATCTTAGAAGCATCTCGCTGCCGGTCCCTGTCCTGGTA
  GAAGACACCAGCAGCAGCAGCGAGTATGGCTCCGTTTCTCCGGACACAGAGCTGAAGGCCGACCCCTTCT
  CCTTCAAAACCAGAGCTAAGTCCTGTGGAGAAAAGGACGGGAAAGGGATCCGGACGCTGTTCCTGGGCAT
  TCCGGATGAAAACTTTGAAGATCACAGTGCGCCCCCGTCCCCTGAAGAGAAAGACTCCGGCTTTTTTATG
  CTGAGAAAGGATAGCGAAAGGCGGGCGACCCTTCACAGGATCCTGACAGAAGACCAGGACAAGGTTGTGA
  GGAACCTAATGGAGTCTCTGGCCCAGGGTCCTGAAGAGCCTAAACTCAAGTGGGAACACATCACAACTCT
  CATCTCAAGCCTCAGAGAGTTTGTGAGGTCCACTGACCGGAAAATCATAGCCACTACACTGTCCAGGCTC
  AAGCTGGAGCTGGACTTCGACAGCCATGGCATCAGCCAGGTGCAGGTGGTCCTCTTTGGCTTCCAGGATG
  CGGTCAATAAAGTTCTCCGGAATCATAACATCAAGCCACACTGGATGTTTGCCTTGGACAGTATCATTCG
  GAAGGCTGTGCAGACAGCTATCACCATCCTGGTTCCAGAGTTGAGGCCACATTTCAGCCTCGCGTCGGAG
  AGCGACACGGCGGATCCGGAAGACTTGGATGTCGACGATGAACACGAGGAATCGCCTTCGAATCAAACCG
  CGCGGAGGCCCCAACCCGTCGCGGAGGATGCCGTGGCTACCTCAGGGGTGAGCACGCTCAGCTCCACCGT
  ATCCCATGATTCCCAGAATGCACACCGCTCGCTGAATGTGCAGCTTGGGAGGATGAAGATGGAAACTAAC
  AGATTACTTGAAGAGTTGGTTCGGAAAGAGAAAGAGTTGCAGGCACTCCTTCATCAAGCCATTGAAGAAA
  AGGACCAAGAAATTAAGCACCTGAGACTTAAGTCCCAACCCATAGATACCCCTGGGTTTCCCGTGTGCCA
  CCTGAACGCTCCTGGTACAGCCATTGAAGACTCTGAACTTACTGGCTGGCTGAGGGAAAATGGAGCTGAC
  GAAGACACTATAAGTCGGTTTCTGGCTGAGGATTACACGCTGGTGGATGTCCTTTACTACGTCACGCGTG
  ACGACTTGAAGTGCCTCCGACTAAGGGGAGGGATGCTGTGCACGCTGTGGAAGGCCATCATTGACTTTCG
  GAACAAAGGGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217609 representing NM_001277694
  Red=Cloning site Green=Tags(s)
  
  MGTEAGEGITFSVPPILSAGFCPIPEGGSCRRGGGAATAAEGEPSLQPLLMPPPPPPPGSFWNVESAAAP
  GTSCPTTAPGSSATRGRGNSGSGGGRRTTVAYVINEASQGQLVVAESEALQSLREACEAVGATLETLHFG
  KLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKN
  TVCTGNYTFIPYMVTPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFVQLLKVAQASSSQYFR
  ESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTF
  DLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEEQVASDMYCLVGRIYKDMFLDSNFTDTESRD
  HGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLG
  ASVLANDHLRVIQASEKLFRLKTPAWYLKSIVETILIYKHFVKLTTEQPTAKQELVDFWMDFLVEATKTD
  VTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFNASSVRGVSISKFEERCCF
  LYVLHNSDDFQIYFCTELHCKRFFEMVNTITEEKGRGAEEGDCEGDSLEYDYEHDENGDRVVLGKGTYGI
  VYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLS
  ALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGIN
  PCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEI
  PESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSGMWLHGEEYLRSISLPVPVLV
  EDTSSSSEYGSVSPDTELKADPFSFKTRAKSCGEKDGKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFM
  LRKDSERRATLHRILTEDQDKVVRNLMESLAQGPEEPKLKWEHITTLISSLREFVRSTDRKIIATTLSRL
  KLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASE
  SDTADPEDLDVDDEHEESPSNQTARRPQPVAEDAVATSGVSTLSSTVSHDSQNAHRSLNVQLGRMKMETN
  RLLEELVRKEKELQALLHQAIEEKDQEIKHLRLKSQPIDTPGFPVCHLNAPGTAIEDSELTGWLRENGAD
  EDTISRFLAEDYTLVDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKGA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277694
• ORF Size: 4143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001277694.1, NP_001264623.1
• RefSeq Size: 4146
• RefSeq ORF: 4146
• Locus ID: 365057
• MW: 154.8 kDa