Arhgap35 (NM_001271132) Rat Tagged ORF Clone

CAT#: RR217647

Arhgap35 (myc-DDK-tagged) - Rat Rho GTPase activating protein 35 (Arhgap35)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap35
• Synonyms: Grlf1; p190RhoGAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217647 representing NM_001271132
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATGGCAAGAAAGCAAGATGTCCGAATCCCCACCTACAACATCAGTGTGGTGGGATTGTCCGGCA
  CTGAGAAGGAGAAAGGCCAGTGCGGCATTGGGAAGTCTTGTCTATGTAACCGCTTTGTGCGCCCAAGTGC
  TGATGAGTTTCACCTGGACCACACTTCTGTCCTCAGCACCAGTGACTTTGGTGGGCGGGTGGTCAATAAT
  GACCATTTTCTGTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGACTGTGTGGAATGTAAGATGCACATTG
  TGGAGCAGACTGAGTTTATTGACGATCAGACTTTTCAACCTCATCGAAGCACGGCACTGCAGCCCTACAT
  CAAGAGAGCCGCAGCCACCAAGCTTGCTTCAGCTGAAAAACTCATGTATTTTTGCACTGACCAGCTAGGG
  CTGGAGCAAGACTTTGAGCAGAAACAGATGCCAGATGGGAAGCTGCTGGTTGACGGTTTCCTTCTGGGCA
  TCGATGTTAGCAGGGGCATGAACAGGAACTTCGATGACCAGCTCAAGTTTGTCTCCAATCTCTACAATCA
  GCTTGCAAAAACAAAAAAGCCCATAGTAATAGTCCTGACCAAGTGTGATGAGGGTGTTGAGCGGTACATT
  AGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTAGAGACCTCTGCAAGGTCCAATG
  TGAATGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAGAGTCGAGGGAAGACGAAAATCAT
  CCCTTACTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAGGACAAGTATGAGTGGTTG
  GTGAGCCGCATTGTGAAAAGTCACAATGAGAACTGGCTGAGTGTCAGCCGAAAGATGCAGGCCTCCCCTG
  AGTACCAGGACTATGTCTATCTGGAAGGGACACAGAAAGCCAAGAAGCTCTTCCTGCAGCACATTCACCG
  CCTCAAGCATGAGCATATTGAGCGCCGGAGAAAGCTGTACCTGGCAGCCCTGCCATTGGCTTTTGAAGCC
  CTCATACCTAATCTAGATGAAGTAGACCACCTGAGCTGCATTAAAGCAAAAAAGCTGTTAGAGACTAAGC
  CAGAGTTCTTAAAGTGGTTTGTTGTACTTGAAGAGACACCATGGGATGAAACCAGCCACATTGACAACAT
  GGAAAATGAGCGGATTCCCTTTGACTTGATGGATACTGTCCCTGCTGAGCAGTTGTATGAGACCCACTTG
  GAGAAGCTGAGGAATGAGAGGAAGAGAGCTGAGATGCGAAGGGCTTTCAAAGAAAACCTGGAGACCTCTC
  CTTTCATAACTCCTGGGAAACCTTGGGAAGAAGCTCGTAGTTTTATTATGAATGAAGACTTCTACCAGTG
  GCTGGAAGAATCTGTGTACATGGACATCTATGGCAAGCACCAAAAGCAGATTATAGACCGGGCAAAGGAA
  GAGTTCCAAGAGTTGCTTTTGGAGTATTCAGAATTGTTTTATGAGCTGGAGCTGGATGCTAAACCCAGTA
  AGGAAAAGATGGGTGTTATCCAGGATGTTCTGGGTGAAGAGCAACGATTTAAAGCATTGCAGAAACTCCA
  AGCAGAGCGTGATGCCCTCATTCTGAAGCACATTCATTTTGTGTACCACCCAACAAAGGAGACATGCCCA
  AGCTGTCCAGCTTGCGTAGATGCTAAGATTGAACATTTGATCAGTTCTCGCTTTATCCGACCATCTGATA
  GGAATCAGAAGAACTCTTTGTCTGACCCCAATATTGATAGGATCAATTTGGTTATTTTAGGCAAAGATGG
  CCTTGCCCGAGAGTTAGCCAATGAAATTCGAGCTCTTTGTACAAATGATGACAAGTATGTAATAGATGGT
  AAAATGTATGAGCTTTCTCTGAGGCCAATAGAGGGGAATGTTCGGCTTCCTGTGAACTCTTTCCAGACAC
  CAACCTTCCAACCCCATGGCTGTCTCTGCCTTTACAATTCAAAGGAGTCGCTGTCCTATGTGGTGGAGAG
  TATAGAGAAGAGCAGAGAATCTACACTGGGCAGGCGGGATAATCACTTAGTCCACCTCCCCTTGACTTTA
  ATTTTAGTTAACAAGAGAGGGGACACAAGTGGAGAGACTCTGCACAGCTTAATACAGCAAGGCCAGCAGA
  TTGCTAGCAAACTTCAGTGTGTCTTTCTTGATCCTGCGTCTGCTGGCATTGGTTATGGACGCAACATTAA
  CGAGAAGCAGATCAGTCAAGTTCTGAAGGGACTCCTGGACTCTAAGCGCAACTTAAACCTGGTTAGTTCT
  ACTGCTAGTATCAAAGATTTGGCTGATGTGGACCTTCGAATTGTCATGTGTCTCATGTGTGGTGATCCTT
  TTAGTGCAGATGACATTCTCTCTCCTGTCCTGCAGTCCCAAACTTGTAAATCTTCCCACTGTGGGAGCAG
  CAACTCTGTTTTACTTGAACTTCCAATTGGAGTACACAAGAAGCGCATTGAGCTGTCTGTTCTTTCATAC
  CATTCCTCATTTAGCATCCGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCAGCCAAACGTA
  AGGCCTCCTTGGCAATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATTATCCCCATCCAGCTTGTCGC
  ACTCACTGATGGCGCTATAGATGTCCTGGACAATGACTTAAGTCGAGAGCAGCTAACAGAGGGAGAGGAA
  ATTGCACAAGAAATTGATGGGAGATTCACAAGCATCCCTTGTAGCCAGCCCCAGCATAAACTCGAGCTCT
  TCCATCCCTTTTTTAAAGATGTGGTGGAGAAAAAGAACATAATCGAGGCCACACACATGTACGATAATGT
  GGCTGAGGCCTGCAGCACCACTGAGGAGGTATTCAACTCCCCCAGGGCTGGGTCACCCCTCTGCAATTCA
  AACTTACAGGACTCAGAAGAAGATGTGGAGCCTCCATCGTACCACCTTTTTCGGGAAGATGCGACATTGC
  CCTCCCTGTCCAAAGATCATTCCAAGTTCTCAATGGAGCTGGAGGGAAACGACGGGCTGTCTTTCATAAT
  GAGCAACTTTGAGAGTAAACTGAACAACAAAGTACCTCCACCAGTCAAACCAAAGCCTCCTGTGCATTTT
  GAGATCACAAAAGATCTTTCTTACTTAGACCAAGGTCATCGGGAGGGACAGAGGAAGTCTATGTCTTCTA
  GCCCCTGGATGCCTCAGGATGGATTTGATCCTTCTGACTACGCAGAGCCCATGGATGCTGTGGTCAAGCC
  AAGGAATGAGGAAGAAAACATATACTCAGTGCCCCACGACAGCACCCAGGGCAAGATCATTACCATTCGG
  AACATCAACAAAGCCCAGTCCAATGGCAGTGGCAATGGTTCTGACAGTGAGATGGACACAAGCTCTCTAG
  AGCGAGGCCGCAAAGTATCTGCAGTGAGTAAGCCTGTGCTGTACAGGACGAGATGCACCCGCCTGGGGCG
  GTTTGCTAGTTACCGCACCAGCTTCAGTGTTGGGAGTGATGATGAGCTGGGACCCATCCGAAAGAAAGAG
  GAGGACCAGGCATCCCAAGGTTATAAAGGGGACAATGCTGTCATTCCTTATGAAACAGATGAGGACCCCA
  GGAGGAGGAATATCCTTCGAAGTCTAAGGAGGAACACCAAGAAACCAAAGCCCAAACCCCGACCATCCAT
  CACAAAGGCAACCTGGGAGAGTAACTATTTTGGGGTGCCTTTAACAACAGTGGTGACTCCAGAGAAGCCG
  ATACCCATTTTCATTGAAAGATGCATTGAGTACATTGAAGCCACAGGACTAAGCACAGAAGGCATCTACC
  GGGTCAGCGGAAACAAGTCAGAAATGGAAAGTTTGCAAAGACAGTTTGATCAAGATCACAATCTGGACCT
  GGCAGAGAAAGACTTCACTGTGAACACTGTGGCAGGGGCCATGAAGAGTTTTTTCTCGGAGCTACCAGAC
  CCCCTGGTACCATACAGCATGCAGATTGACTTGGTGGAAGCTCACAAGATCAACGACAGGGAGCAGAAGC
  TGCATGCTCTGAAGGAAGTGCTGAAGAAGTTCCCTAAGGAGAACCATGAAGTCTTCAAATATGTCATCTC
  CCACCTGAACAGAGTCAGCCACAACAACAAGGTGAATCTTATGACCAGTGAGAACCTGTCCATCTGCTTC
  TGGCCCACGTTGATGCGGCCTGACTTCAGCAGCATGGACGCACTCACAGCCACTCGATCCTACCAGACCA
  TCATCGAGCTCTTCATCCAGCAGTGCCCCTTCTTCTTCTACAACCGGCCGATCAGTGAGCCACCGGGGGC
  TGCGCCTGGCTCCCCTTCAGCCATGGCACCCACTGTCCCCTTCCTCACCTCTACACCTGCTACCAGTCAG
  CCATCACCTCCCCAGTCACCTCCTCCAACCCCTCAGTCCCCAATGCAGCCATTGCTCTCCTCTCAGCTCC
  AAGCCGAACACACGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217647 representing NM_001271132
  Red=Cloning site Green=Tags(s)
  
  MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNN
  DHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLG
  LEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVIVLTKCDEGVERYI
  RDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWL
  VSRIVKSHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEA
  LIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDETSHIDNMENERIPFDLMDTVPAEQLYETHL
  EKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKE
  EFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCP
  SCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDG
  KMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTL
  ILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSS
  TASIKDLADVDLRIVMCLMCGDPFSADDILSPVLQSQTCKSSHCGSSNSVLLELPIGVHKKRIELSVLSY
  HSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEE
  IAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNS
  NLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHF
  EITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIR
  NINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKE
  EDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKP
  IPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPD
  PLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNRVSHNNKVNLMTSENLSICF
  WPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQ
  PSPPQSPPPTPQSPMQPLLSSQLQAEHTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271132
• ORF Size: 4497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271132.1, NP_001258061.1
• RefSeq Size: 8364
• RefSeq ORF: 4500
• Locus ID: 306400
• MW: 170.5 kDa