Psme4 (NM_001025140) Rat Tagged ORF Clone

CAT#: RR217697

Psme4 (myc-DDK-tagged) - Rat proteasome (prosome, macropain) activator subunit 4 (Psme4)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psme4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217697 representing NM_001025140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTCCGGAGCGGGCGGGTGGCGGGGAGCCCCCCGAGCCTGGCGGGCGGCCGGTACTGGGCCCGC
  GGGCCTTCGTCCCGCAGAAAGAGATCGTGTACAACAAGCTTCTGCCCTACGCCGAGCGGCTGGACGCCGA
  GTCCGACTTGCAGCTGGCGCAGATCAAGAGCAACCTGGGCCGGGCAGTGCAGCTCCAGGAGCTGTGGCCG
  GGCGGCCTCTTCTGGACGCGGAAACTCTCCACTTATATTCGACTTTATGGGAGAAAATTTAGCAAAGAAG
  ATCATGTTCTTTTTATTAAGTTATTGTATGAGCTGGTATCAATTCCAAAACTAGAAATCAGCATGATGCA
  GGGATTTGCCCGTCTCTTGATCAACTTATTAAAAAAAAAGGAACTTCTTTCAAGAGATGATTTAGAGTTA
  CCATGGAGACCACTTTATGACATGGTAGAAAGAATATTATATTCCAAGACAGAGCACCTGAGATTAAATT
  CATTTCCTAATTCTATAGAAAATGTTCTCAAAACCCTCGTGAAAAGCTGCCGACCATATTTTCCAGCAGA
  TGCAACAGCAGAAATGTTAGAAGAATGGAGGCCTTTAATGTGTCCTTTTGATGTAACAATGCAAAAGGCC
  GTCAGTTATTTTGAAATATTTCTTCCTACCTCCCTTCCTCCAGAACTGCATCATAAAGGTTTTAAACTTT
  GGTTTGATGAATTAATTGGCCTTTGGGTTTCAGTGCAAAATCTCCCACAGTGGGAGGGGCAATTAGTAAA
  TCTCTTTGCTCGATTGGCTACAGATAATATAGGATACATAGATTGGGATCCATATGTACCAAAGATATTT
  ACAAGAATTCTGAGAAGCCTGAACCTCCCAGTGGGAAGTAGTCAAGTGTTAGTCCCAAGATTTTTAACAA
  ATGCTTATGATATAGGACATGCTGTAATATGGATCACCGCAATGATGGGTGGACCAAGTAAATTAGTACA
  AAAACATTTGGCTGGTCTGTTTAACAGCATCACATCTTTTTATCATCCTTCAAATAATGGGCGTTGGCTG
  AACAAGTTAATGAAACTCCTTCAGCGGTTGCCAAATAGTGTTGTTAGGAGATTACATCGTGAAAGATTCA
  AGAAGCCCTCCTGGTTAACTCCTGTGCCTGAAAGCCATAAGCTTACGGATGAAGATGTTACAGACTTTGT
  ACAATGCATTATTCAGCCTGTCCTCTTGGCTATGTTCAGCAAAACTGGTAGTCTAGAAGCAGCCCAGGCG
  CTGCAGAACCTTGCACTCATGAGACCGGAGTTAGTAATACCTCCTGTGCTTGAAAGAACATACCCTGCTT
  TAGAGACATTAACAGAACCTCACCAGCTCACAGCTACTTTAAATTGTGTAATTGGAGTAGCCCGAAGTTT
  GGTATCAGGCAGCAAATGGTTTCCTGAAGGTCCAACACACATGCTACCTCTGTTGATGAGAGCATTGCCT
  GGGGTAGATCCAAACGACTTCAGTAAATGCATGATCACATTCCAATTTATAGGAACATTTTCTACTCTGG
  TGCCTTTAGTAGACTGTTCATCTGTACTGCAAGAAAGAAATGACCTCACAGAAATTGAAAAAGAACTTTG
  TTCAGCAACAGCTGAATTTGAGGATTTTGTGTTACAATTTATGGACAGATGCTTTGGACTAATAGAAAGT
  AGCACATTGGAGCAAACAAGGGAAGAGACAGAAACAGAGAAAATGACTCATTTGGAGAGTTTGGTTGAAT
  TAGGCTTGTCTTCTACGTTTAGTACAATCCTCACACAATGTTCCAAAGACATATTTATGGTAGCCCTTCA
  GAAGGTCTTTAACTTTTCTGTCTCACATATATTTGAAACAAGAGTTGCAGGTCGCATGGTGGCAGACATG
  TGCCGAGCTGCTGTAAAGTGTTGCCCGGAAGAATCCCTGAAGCTCTTTGTTCCCCACTGCTATGGTGTTA
  TAACTCAGCTTACAATGAATGATGATGTACTGAATGAAGAAGAGCTAGACAAGGAGTTGCTATGGAACCT
  TCAGCTTCTGTCTGAGATTACTCGTGTGGATGGGAAGAAGTTACTTCTATATAGGGAGCAGCTTGTAAAG
  ATTCTCCAACGAACCTTACACCTGACCTGTAAGCAAGGCTACACACTGTCTTGCAACCTTTTGCATCATC
  TTCTTCGTTCTACGACACTAATCTACCCCACAGAGTACTGCAGTGTTCCAGGTGGCTTTAACAAGCCGCC
  TTCTGAATACTTCCCTATCAAGGACTGGGGAAAGCCTGGAGATCTGTGGAATCTGGGGATACAGTGGCAT
  GTTCCTTCTTCAGAGGAGGTCTCTTTTGCCTTCTATTTGTTACAATCCTTTCTTCAGCCTGAGCTTATCA
  AACTCCAGCGTTGTGGAGATGGAGAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGT
  ACACAACTGCTTGATTGGTTCAGGGAACCTTCTACCTCCACTGAAAGGAGAGCCAGTTACTAACTTGGTA
  CCAAGTATGGTGTCCTTAGAGGAAACAAAATTGTATACCGGACTGGAACATGACCTATCCAAAGAAAACC
  ACCGAGAAGTAATTGCTAGTGTCATAAGGAAACTTCTTAGCCACATACTTGATAATTCAGAAGATGATAC
  CAAATCGTTGTTTCTTATCATAAAGATCATTGGAGACCTTCTGCAATTCCAAGGATCTCATAAACATGAA
  TTTGACTCTCGCTGGAAAAGCTTTAATTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGAAAAAAGC
  AACATATCAGAGCACTACTAATTGACAGAGTGATGCTGCAGCACGAGTTGCGAACACTAACTGTTGAGGG
  TTGTGAGTACAAAAAAATACATCAAGATATGATCAGAGATCTTCTTCGATTATCAACAAGTTCATACAGT
  CAGGTCAGAAACAAAGCTCAGCAAACATTTTTTGCTGCTTTAGGAGCCTATAACTTCTGTTGCAGAGATA
  TCATCCCCTTGGTTTTGGAGTTCTTACGGCCTGATAGAAAGGATGTCACACAGCAGCAGTTCAAGGGCGC
  CCTATATTGTCTACTTGGAAATCACAGTGGCGTGTGCCTGGCAAACCTTCATGACTGGGACTGTATTGTG
  CAGACTTGGCCAGCACTTGTTTCTTCAGGACTTAGCCAAGCAATGTCACTGGAAAAGCCATCAATAGTGA
  GACTATTTGATGACCTTGCTGAAAAGATTCATAGACAATATGAAACAATTGGCTTAGACTTCACAATTCC
  AAAGTCATGTGCTGCAATAGCAGAATTACTTCAACAATCAAAGAATCCTTCCATTAGCCAGACACTACTT
  AGCCCAGAAAAGATTAAAGAAGGACAGAAGCGTCAACAGGATAAGAATGCTGATGCTGTAAGGAACTATG
  AATGTTTGGTAAATACTTTACTAGATGGTGTGGAGCAAAGAAATCTGCCTTGGAAATTTGAGCATATAGG
  AATCGGGCTTCTGTCTTTACTCCTAAGGGATGACAGAGTGTTGCCTCTTCGTGCCATACGTTTTTTTGTT
  GAAAATCTCAACCATGATGCAATCGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGC
  AGCTAAAGAGAACTCACAAAAAGCTGACCATCAACCCTTATGAAATCAGTGGATGTCCTAAACCTGCCAC
  AGTTCTTGCTGGTGATAGACCTGATAACCATTGGTTGCATTATGATAGCAAAAATATACCAAGAACAAAA
  AAAGAATGGGAGTCAAGTTGCTTTGTTGAAAAAACTCACTGGGGATACTACAACTGGCCAAAGAACATGG
  TTGTTTATGCTGGTGTGGAAGAGCAGCCTAAACTTGGCAGAAGCAGGGAGGACATGACAGAGGCAGAACA
  GATTATATATGATCGTTTTTCTGACCCTAAGTTTGTTGAGCAGTTAATCACATTTCTATCTTTAGAAGAC
  AGAAAAGGAAGAGATAAGTTTAGTCCCCGGCGCTTCTGCCTCTTTAAGGGTATATTTAGAAATTTTGACG
  ATGCTTTTTTGCCTGTTCTGAAGCCCCATTTAGAACGTTTGGTTGCAGATTCTCATGAAAGTACCCAGAG
  ATGTGTTGCAGAAATTATAGCTGGTTTAATCAGAGGTTCTAAGCATTGGACGTTTGAGAAGGTGGAGAAG
  CTTTGGGAGCTCCTATGTCCGCTGCTGAGAACAGCACTGTCCAACATGACAGTGGAGACGTACAATGACT
  GGGGAACCTGTATAGCCACATCCTGTGAAAGCAGAGATCCCCGGAAGCTTCATTGGCTTTTTGAGCTGCT
  CTTGGAGTCACCATTGAGTGGTGAAGGGGGGTCCTTTGTAGATGCATGCCGACTCTATGTGCTACAAGGT
  GGCCTTGCCCAGCAAGAATGGAGAGTCCCTGAACTATTGCACAGACTTCTGAAGTACCTGGAACCGAAAC
  TCACCCAGGTTTACAAAAATGTCAGAGAAAGGATAGGGAGTGTTCTGACCTACATATTCATGATCGATGT
  TTCTTTGCCAAATACTGCTCCAACAACATCTCCTTGTATCCCTGAGTTTACTGCGCGAGTCCTAGAAAAA
  CTGAAACCTCTCACAGATGTGGATGAAGAAATTCAGAACCATGTCATGGAAGAAAATGGCATTGGTGAAG
  AAGATGAACGGACTCAGGGCATTAAACTCTTAAAAACCATATTGAAATGGCTTATGGCAAGTGCAGGAAG
  GTCATTTTCTACAGCAGTCAAAGAGCAGCTTCAGCTTCTGCCTCTGTTCTTTAAGATCGCCCCAGTGGAA
  AATGACAATAGTTATGATGAGTTGAAAAGAGATGCAAAGCTCTGTTTATCACTAATGTCTCAGGGGTTGC
  TCTACCCTCAGCAAGTGCCTTTGATACTTCAGGTGCTAAGCCAGACAGCAAGAAGCAGTTCTTGGCATGC
  AAGATATACAGTGCTGACCTACCTCCAGACCATGGTATTTTATAACCTCTTTATTTTCCTAAACAATGAA
  GATGCAGTTAAAGACATCAGATGGCTGATTATACGCCTTTTGGAGGATGAACAGTTAGAGGTTCGAGAAA
  TGGCTGCTACTACCTTAAGTGGTTTGTTACAGTGTAACTTCCTTACCATGGACAGTGCTATGCAGAGTCA
  TTTTGAGCAACTTTGCAAAACTAAACTACCCAAGAAAAGAAAACGAGACCCTGGTTCTGTGGGAGATACC
  ATTCCTTCTGCAGAGTTGGTCAAACGTCATGCTGGTGTGCTTGGACTTGGTGCATGTGTTCTTTCTAGCC
  CTTATGATGTTCCTACCTGGATGCCCCAGCTCCTTATGAATCTCAGTGCACATTTGAATGATCCTCAGCC
  TATTGAAATGACTGTCAAGAAGACCTTATCCAATTTCCGAAGGACACATCATGACAACTGGCAAGAACAC
  AAGCAGCAGTTCACTGATGACCAGCTGCTTGTCCTCACTGACCTCCTTGTGTCACCATGCTATTATGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217697 representing NM_001025140
  Red=Cloning site Green=Tags(s)
  
  MEAPERAGGGEPPEPGGRPVLGPRAFVPQKEIVYNKLLPYAERLDAESDLQLAQIKSNLGRAVQLQELWP
  GGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRDDLEL
  PWRPLYDMVERILYSKTEHLRLNSFPNSIENVLKTLVKSCRPYFPADATAEMLEEWRPLMCPFDVTMQKA
  VSYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIF
  TRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWL
  NKLMKLLQRLPNSVVRRLHRERFKKPSWLTPVPESHKLTDEDVTDFVQCIIQPVLLAMFSKTGSLEAAQA
  LQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPEGPTHMLPLLMRALP
  GVDPNDFSKCMITFQFIGTFSTLVPLVDCSSVLQERNDLTEIEKELCSATAEFEDFVLQFMDRCFGLIES
  STLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVAGRMVADM
  CRAAVKCCPEESLKLFVPHCYGVITQLTMNDDVLNEEELDKELLWNLQLLSEITRVDGKKLLLYREQLVK
  ILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPSEYFPIKDWGKPGDLWNLGIQWH
  VPSSEEVSFAFYLLQSFLQPELIKLQRCGDGELEMSRDDILQSLTIVHNCLIGSGNLLPPLKGEPVTNLV
  PSMVSLEETKLYTGLEHDLSKENHREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLQFQGSHKHE
  FDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYS
  QVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIV
  QTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLL
  SPEKIKEGQKRQQDKNADAVRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFV
  ENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEISGCPKPATVLAGDRPDNHWLHYDSKNIPRTK
  KEWESSCFVEKTHWGYYNWPKNMVVYAGVEEQPKLGRSREDMTEAEQIIYDRFSDPKFVEQLITFLSLED
  RKGRDKFSPRRFCLFKGIFRNFDDAFLPVLKPHLERLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEK
  LWELLCPLLRTALSNMTVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQG
  GLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTAPTTSPCIPEFTARVLEK
  LKPLTDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVKEQLQLLPLFFKIAPVE
  NDNSYDELKRDAKLCLSLMSQGLLYPQQVPLILQVLSQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNE
  DAVKDIRWLIIRLLEDEQLEVREMAATTLSGLLQCNFLTMDSAMQSHFEQLCKTKLPKKRKRDPGSVGDT
  IPSAELVKRHAGVLGLGACVLSSPYDVPTWMPQLLMNLSAHLNDPQPIEMTVKKTLSNFRRTHHDNWQEH
  KQQFTDDQLLVLTDLLVSPCYYA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025140
• ORF Size: 5529 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025140.2, NP_001020311.2
• RefSeq Size: 7327 bp
• RefSeq ORF: 5532 bp
• Locus ID: 498433
• Cytogenetics: 14q22
• MW: 211.2 kDa