Prpf8 (NM_001191590) Rat Tagged ORF Clone

CAT#: RR217763

Prpf8 (myc-DDK-tagged) - Rat pre-mRNA processing factor 8 (Prpf8)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prpf8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217763 representing NM_001191590
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGAGTGTTTCCTTACAGAGGGCCGGGTAACCCGGTGCCCGGCCCTCTAGCCCCGCTGCCGGATT
  ACATGTCAGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAGTTGCAGGCCAAGCGCTATGCAGA
  GAAGCGGAAATTCGGGTTTGTGGATGCTCAGAAGGAAGACATGCCCCCAGAACATGTCAGGAAAATCATT
  CGAGACCATGGAGACATGACCAACAGGAAGTTCCGTCATGACAAAAGGGTCTATTTGGGAGCTCTCAAGT
  ACATGCCTCACGCAGTTCTCAAGCTCCTAGAGAACATGCCCATGCCTTGGGAGCAGATCCGGGATGTGCC
  TGTGCTGTACCACATCACTGGAGCCATTTCCTTCGTTAACGAGATTCCCTGGGTTATTGAGCCAGTTTAC
  ATCTCCCAGTGGGGGTCAATGTGGATCATGATGCGTAGAGAAAAAAGAGACAGGAGGCATTTCAAGAGGA
  TGCGTTTTCCCCCTTTTGATGATGAGGAGCCGCCCCTGGATTATGCTGACAACATCTTAGATGTCGAGCC
  ACTGGAAGCCATTCAGCTAGAGCTGGACCCTGAGGAGGATGCCCCTGTGTTGGACTGGTTCTATGACCAC
  CAGCCATTGAGAGATAGTAGGAAGTATGTTAATGGTTCCACCTACCAACGCTGGCAGTTCACATTGCCTA
  TGATGTCCACTCTCTACCGACTGGCCAACCAGCTTCTGACTGACTTGGTAGATGACAACTACTTTTATCT
  CTTTGATCTAAAGGCCTTCTTTACCTCTAAGGCACTCAACATGGCCATTCCTGGGGGACCCAAATTTGAA
  CCTCTTGTTCGAGACATCAACCTACAGGATGAAGACTGGAATGAATTCAATGATATTAACAAGATCATCA
  TCCGTCAGCCTATTCGTACTGAGTACAAGATTGCTTTTCCGTACCTCTATAACAACCTCCCACACCATGT
  CCACCTGACCTGGTACCACACTCCTAACGTTGTGTTCATCAAGACTGAGGATCCTGATCTGCCAGCTTTC
  TACTTTGATCCTCTGATTAACCCAATCTCCCATAGACACTCAGTCAAGAGCCAAGAGCCACTACCTGATG
  ATGATGAGGAGTTTGAGCTCCCAGAGTTTGTGGAGCCCTTCCTGAAGGATACACCTCTCTATACAGACAA
  TACAGCCAATGGCATCGCACTGCTCTGGGCACCACGGCCCTTCAACCTACGCTCTGGTCGTACCCGCCGG
  GCCCTCGACATTCCCCTTGTCAAGAACTGGTATCGGGAGCACTGTCCTGCTGGGCAACCTGTGAAAGTTC
  GAGTCTCCTATCAGAAGCTGCTTAAGTATTATGTGCTGAATGCCCTCAAACATCGGCCTCCCAAAGCGCA
  GAAGAAGAGGTATCTGTTTCGCTCTTTCAAAGCTACCAAATTCTTTCAGTCTACCAAGCTGGACTGGGTG
  GAGGTCGGGCTGCAGGTTTGCCGCCAGGGCTACAACATGCTTAACCTTCTCATTCACCGTAAGAACCTCA
  ACTATCTGCATTTGGACTATAACTTCAACCTGAAGCCTGTCAAGACGCTCACCACCAAGGAAAGAAAAAA
  ATCTCGCTTTGGGAATGCTTTCCATCTGTGTCGGGAAGTACTGCGTTTGACTAAGCTGGTTGTGGATAGT
  CATGTGCAGTATCGCTTGGGCAATGTAGATGCCTTCCAGCTGGCAGATGGATTGCAGTATATATTTGCCC
  ACGTGGGACAGTTGACAGGCATGTATCGATACAAATATAAGCTGATGCGGCAGATCCGCATGTGCAAGGA
  CTTGAAACATCTCATCTATTACCGCTTCAACACGGGTCCTGTGGGGAAAGGCCCTGGCTGTGGTTTCTGG
  GCTGCTGGCTGGCGGGTCTGGCTATTTTTTATGCGTGGAATTACCCCTTTGCTAGAACGATGGCTAGGCA
  ACCTTCTGGCCCGACAGTTTGAAGGCCGTCACTCAAAGGGGGTGGCAAAGACAGTAACAAAGCAACGAGT
  AGAGTCCCATTTTGACCTTGAGCTTAGGGCAGCTGTGATGCATGATATTCTGGACATGATGCCTGAAGGC
  ATCAAACAGAACAAGGCCCGAACAATTCTGCAGCACCTCAGTGAGGCCTGGCGCTGCTGGAAGGCCAACA
  TTCCATGGAAGGTCCCAGGACTGCCAACGCCTATAGAGAATATGATTCTTCGGTACGTTAAGGCCAAGGC
  TGATTGGTGGACCAATACTGCCCACTACAACCGTGAACGGATTCGCCGTGGGGCTACTGTAGACAAGACT
  GTTTGCAAAAAGAACCTGGGCCGCCTCACCCGCCTGTATCTGAAGGCAGAACAGGAGCGGCAGCACAATT
  ACCTGAAGGACGGGCCTTACATCACAGCAGAGGAAGCAGTGGCAGTGTATACTACCACTGTGCACTGGCT
  GGAAAGCCGTAGGTTCTCTCCTATCCCGTTCCCCCCACTCTCCTACAAGCATGACACCAAGTTGCTTATC
  TTGGCTCTGGAGCGACTCAAGGAAGCTTATAGTGTGAAGTCTAGGTTAAACCAGTCTCAGAGGGAGGAGC
  TGGGTCTGATTGAACAAGCCTATGACAACCCCCATGAGGCTTTGTCCCGCATCAAGCGTCACCTCCTCAC
  GCAGAGAGCCTTTAAGGAAGTGGGCATTGAGTTTATGGATCTCTATAGCCACCTTGTGCCAGTGTATGAT
  GTGGAACCACTGGAGAAAATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCAGATAAACGCC
  GTCTTTTCCCGCCCTGGATCAAGCCTGCTGACACAGAACCCCCTCCACTGCTTGTTTACAAGTGGTGCCA
  AGGCATCAATAACCTGCAGGATGTGTGGGAGACGAGTGAGGGTGAGTGCAATGTCATGCTGGAGTCTCGG
  TTTGAGAAGATGTATGAGAAGATTGACCTGACCCTGCTCAACAGGCTGCTGCGACTCATTGTGGACCACA
  ACATAGCTGACTACATGACAGCCAAGAACAATGTAGTCATCAACTATAAGGACATGAACCATACAAATTC
  ATATGGAATCATCAGAGGCCTGCAGTTTGCCTCATTCATTGTACAGTACTATGGTTTGGTGATGGATTTG
  CTTGTATTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCTCCGCAGATGCCAAATGACTTTCTCAGTT
  TTCAGGACATAGCCACCGAGGCTGCACACCCAATCCGACTCTTCTGTAGATACATTGATCGCATCCATAT
  TTTCTTCAGGTTCACAGCAGATGAGGCTCGGGATCTGATCCAGCGTTACCTGACAGAACATCCAGACCCC
  AATAATGAAAACATTGTTGGCTACAATAATAAGAAATGCTGGCCCCGAGATGCCCGTATGCGTCTCATGA
  AGCATGATGTCAACTTGGGTCGGGCAGTGTTCTGGGACATCAAAAACCGGCTGCCACGATCGGTGACTAC
  AGTACAGTGGGAGAATAGCTTTGTGTCTGTGTACAGTAAAGACAACCCCAACCTGCTGTTCAACATGTGT
  GGCTTTGAGTGCCGCATCCTACCTAAGTGCCGTACCAGCTATGAAGAGTTCACCCACAAGGACGGGGTCT
  GGAATCTACAGAATGAGGTTACTAAGGAGCGGACGGCTCAGTGTTTCCTGCGTGTGGACGATGAGTCAAT
  GCAGCGCTTCCATAACCGCGTGCGGCAGATTCTGATGGCCTCTGGCTCTACCACTTTCACCAAGATTGTG
  AATAAGTGGAATACAGCTCTCATTGGCCTTATGACATACTTTCGAGAGGCTGTGGTGAATACACAGGAAC
  TCCTAGACTTACTGGTGAAGTGTGAAAACAAGATCCAGACACGTATCAAGATTGGATTGAACTCCAAGAT
  GCCAAGTCGGTTCCCCCCTGTTGTATTCTACACCCCTAAGGAGCTGGGTGGGCTTGGCATGCTCTCGATG
  GGCCATGTCCTTATCCCCCAGTCTGACCTCAGGTGGTCCAAGCAAACAGATGTGGGCATCACACACTTTC
  GTTCAGGAATGAGTCATGAAGAAGACCAACTGATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAG
  TGAATTCATTGACTCCCAGCGGGTCTGGGCTGAGTATGCACTCAAGCGACAGGAGGCTATTGCTCAGAAC
  AGACGCTTGACTTTAGAAGATTTAGAAGACTCGTGGGATCGGGGCATTCCCCGGATCAATACCCTCTTCC
  AGAAAGATCGGCACACATTGGCTTATGATAAAGGCTGGCGTGTCCGAACTGACTTTAAGCAGTACCAGGT
  TTTGAAGCAGAACCCCTTTTGGTGGACACATCAACGGCATGATGGCAAGCTTTGGAACCTCAACAACTAC
  CGCACAGACATGATCCAGGCACTGGGCGGTGTGGAGGGCATTCTGGAACACACTCTCTTCAAGGGCACTT
  ACTTCCCAACCTGGGAAGGTCTGTTCTGGGAGAAAGCCAGCGGGTTTGAGGAATCCATGAAGTGGAAGAA
  GCTGACCAATGCTCAGCGATCAGGATTAAACCAGATTCCTAATCGTAGATTTACTCTCTGGTGGTCCCCA
  ACAATCAATCGAGCCAATGTATACGTAGGCTTCCAGGTGCAGCTGGACCTGACAGGAATCTTTATGCATG
  GCAAGATCCCCACGCTGAAGATCTCTCTCATCCAGATCTTCCGAGCTCACTTGTGGCAAAAGATTCATGA
  GAGCATCGTGATGGACTTGTGTCAGGTCTTCGACCAGGAATTAGATGCCCTGGAAATTGAGACAGTACAA
  AAGGAGACAATCCATCCCCGAAAATCCTACAAGATGAACTCTTCCTGTGCAGATATACTGCTCTTTGCTT
  CCTATAAGTGGAATGTCTCCCGGCCCTCGCTGCTGGCAGACTCTAAGGATGTGATGGACAGCACCACCAC
  ACAGAAGTATTGGATTGACATCCAGTTACGCTGGGGGGACTATGATTCCCATGATATTGAGCGCTATGCC
  CGGGCCAAGTTCCTGGATTACACCACAGACAACATGAGTATCTACCCTTCACCCACAGGTGTGCTCATTG
  CCATTGATCTGGCCTATAATCTACACAGTGCCTATGGAAACTGGTTCCCGGGGAGCAAGCCTCTCATACA
  GCAAGCCATGGCCAAGATCATGAAGGCAAACCCTGCCTTGTATGTGTTACGTGAACGGATACGCAAAGGA
  TTGCAGCTGTATTCATCTGAGCCCACTGAGCCTTACCTGTCCTCTCAGAACTATGGTGAACTCTTCTCGA
  ACCAGATTATCTGGTTTGTGGATGACACCAATGTCTATAGAGTAACTATCCACAAGACCTTTGAGGGGAA
  CTTGACAACCAAACCCATCAATGGGGCCATCTTCATCTTCAATCCACGCACAGGACAGCTGTTCCTCAAG
  ATAATCCACACATCCGTATGGGCTGGACAGAAGCGTTTGGGTCAGTTGGCCAAGTGGAAGACTGCTGAAG
  AAGTGGCTGCCCTGATCCGATCTCTGCCTGTGGAGGAACAGCCTAAACAGATTATTGTCACTCGGAAAGG
  GATGCTGGATCCCCTGGAGGTACACTTACTGGACTTCCCCAATATTGTGATCAAAGGATCAGAGCTCCAG
  CTGCCCTTCCAGGCTTGTCTCAAGGTGGAGAAGTTTGGGGATCTCATCCTTAAAGCCACAGAACCACAGA
  TGGTTCTCTTCAACCTCTATGATGACTGGCTCAAGACTATCTCATCCTACACGGCTTTCTCCCGTCTCAT
  CCTGATCCTGCGTGCTTTGCATGTGAACAACGATAGGGCAAAAGTGATCCTGAAGCCAGACAAGACAACC
  GTTACGGAACCCCACCACATCTGGCCCACGCTCACTGACGAGGAGTGGATCAAGGTGGAGGTGCAGCTCA
  AGGATCTGATCTTGGCTGACTATGGCAAGAAAAACAATGTGAATGTGGCATCGCTGACACAATCAGAAAT
  TCGAGACATCATCCTGGGAATGGAGATCTCTGCACCATCGCAGCAGCGGCAGCAGATAGCTGAGATTGAG
  AAGCAGACCAAGGAACAGTCCCAGCTCACTGCTACACAGACTCGAACTGTCAACAAGCATGGCGATGAGA
  TCATCACCTCCACCACTAGCAACTATGAGACCCAGACCTTCTCGTCGAAGACCGAATGGAGAGTCAGGGC
  CATCTCTGCTGCCAACCTGCATCTACGGACCAATCACATTTATGTTTCATCTGATGACATCAAGGAGACT
  GGTTATACGTACATTCTTCCCAAAAATGTGCTGAAGAAGTTCATCTGCATATCTGATCTGCGCGCCCAAA
  TTGCAGGATACCTATATGGGGTGAGCCCTCCAGATAACCCTCAGGTGAAGGAGATCCGCTGTATTGTGAT
  GGTGCCACAGTGGGGCACTCACCAGACTGTGCACCTGCCTAGTCAGCTGCCTCAACATGAGTACCTCAAG
  GAGATGGAACCCTTAGGTTGGATCCACACTCAACCCAATGAATCCCCCCAGCTATCGCCCCAAGATGTAA
  CTACTCATGCCAAAATCATGGCTGACAACCCATCTTGGGATGGGGAGAAGACTATTATCATCACCTGCAG
  CTTCACACCAGGTTCCTGCACACTGACAGCCTACAAGCTGACCCCTAGCGGGTATGAGTGGGGTCGGCAG
  AACACAGACAAAGGCAACAACCCCAAAGGCTACCTACCTTCGCACTATGAGAGGGTTCAGATGCTCCTGT
  CTGACCGATTCCTCGGCTTCTTTATGGTCCCTGCCCAGTCCTCCTGGAACTACAACTTTATGGGTGTTCG
  ACATGACCCCAACATGAAATACGAGCTGCAGCTAGCAAACCCCAAGGAGTTCTACCATGAGGTCCACAGG
  CCCTCCCACTTCCTCAACTTTGCCCTCCTGCAGGAGGGTGAGGTCTATTCCGCAGACAGAGAGGACCTCT
  ATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217763 representing NM_001191590
  Red=Cloning site Green=Tags(s)
  
  MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKII
  RDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVY
  ISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDH
  QPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFE
  PLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAF
  YFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
  ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWV
  EVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDS
  HVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFW
  AAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEG
  IKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKT
  VCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
  LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYD
  VEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESR
  FEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDL
  LVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDP
  NNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMC
  GFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
  NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSM
  GHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQN
  RRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNY
  RTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSP
  TINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQ
  KETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
  RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKG
  LQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLK
  IIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQ
  LPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTT
  VTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIE
  KQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
  GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLK
  EMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQ
  NTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHR
  PSHFLNFALLQEGEVYSADREDLYA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191590
• ORF Size: 7005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001191590.1, NP_001178519.1
• RefSeq Size: 7246
• RefSeq ORF: 7008
• Locus ID: 287530
• MW: 273.6 kDa