Cacna1b (NM_001195199) Rat Tagged ORF Clone

CAT#: RR217766

Cacna1b (myc-DDK-tagged) - Rat calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 1

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1b
• Synonyms: BIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217766 representing NM_001195199
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGCG
  GCGGGGCCGGCGGGGCCGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA
  GTCCATTGCGCAACGCGCACGGACCATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC
  GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATATGCTAAGCGCATCACCGAAT
  GGCCGCCCTTCGAATATATGATCCTGGCCACCATCATCGCCAACTGTATTGTCCTGGCCCTGGAGCAGCA
  CCTCCCTGATGGGGACAAGACTCCCATGTCTGAACGACTGGATGACACGGAACCTTACTTCATCGGCATC
  TTTTGCTTCGAGGCGGGCATCAAGATCATAGCTCTGGGCTTCGTGTTCCACAAAGGCTCCTACCTCCGGA
  ATGGCTGGAACGTCATGGACTTCGTGGTGGTCCTCACAGGGATTCTTGCCACAGCTGGAACTGACTTTGA
  TCTGCGCACCCTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGTTGGTGTCTGGAATTCCAAGCTTGCAG
  GTGGTGCTCAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATCGGGCTGCTGCTCTTCTTCGCCA
  TCCTCATGTTCGCTATCATCGGCCTCGAGTTCTATATGGGCAAATTCCATAAGGCCTGCTTCCCCAACAG
  CACAGATGCAGAGCCTGTGGGTGACTTTCCTTGTGGCAAGGAGGCCCCTGCTCGTCTGTGTGACAGTGAC
  ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA
  TCTTGACCGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC
  CGGCAACACGTGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG
  GTGCTCGGTGTGCTTTCAGGAGAGTTTGCCAAAGAGCGGGAGCGAGTCGAGAACCGCCGTGCCTTCCTGA
  AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAACTGAATGGGTACTTGGAGTGGATCTTCAAGGCGGAGGA
  AGTCATGTTGGCAGAGGAGGACAAGAACGCAGAAGAGAAGTCCCCTTTGGATGCAGTGTTGAAGAGAGCT
  GCTACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTG
  CTGCTGGGTCTCCCTTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACAGAGAGCTCATCGTACTTCCGGAG
  GAAGGAGAAGATGTTCCGGTTCCTTATCCGTCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTG
  TGCGTGGTGGCCCTGAACACGTTGTGTGTGGCCATGGTACACTATAATCAGCCTCAGCGGCTTACCACTG
  CACTGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTACGGTCT
  AGGGCCCAGAAGCTACTTCCGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTT
  GAAGTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTCGGAATCAGTGTGCTGCGGGCTCTCCGACTGC
  TGAGGATTTTCAAAGTCACCAAGTATTGGAACTCCCTGAGGAACCTGGTTGTTTCCCTCCTCAACTCCAT
  GAAGTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTTTTCATTGTGGTCTTCGCTCTGTTGGGGATGCAG
  CTGTTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCA
  TCCTCACTGTGTTTCAGATTCTGACAGGAGAGGACTGGAATGCAGTCATGTATCATGGGATTGAGTCACA
  AGGAGGAGTCAGCAAAGGCATGTTTTCATCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACC
  CTGTTGAACGTTTTCTTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAG
  AGGAGATGGAAGAGGCAGCCAATCAGAAGCTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCC
  CATGTCTGCTGCCAACATCTCCATTGCTGCTTTTGTAAAGCAAACTCGAGGTACTGTATCTCGCAGCTCA
  TCTGTCTCCAGCGTAAACTCACCGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAGCGGGCCA
  GTCAGCTAAGGCTCCAGAACCTGCGTGCCAGCTGTGAGGCACTGTACAGTGAGATGGACCCGGAGGAGCG
  CCTGCGTTATGCCAGCACGCGCCACGTGAGGCCAGACATGAAGACACACATGGACCGACCCCTAGTGGTG
  GAACCTGGTCGGGATGGCCTGCGGGGACCCGCCGGGAACAAGTCAAAGCCTGAGGGCACGGAGGCCACCG
  AAGGTGCGGATCCACCACGCCGACACCACCGGCATCGTGATAGGGACAAGACCTCAGCCTCAACCCCTGC
  TGGAGGCGAACAGGACAGGACAGACTGCCCAAAGGCCGAAAGCACCGAGACCGGGGCCCGGGAGGAACGT
  GCGCGCCCTCGTCGAAGTCACAGCAAGGAGGCTCCAGGGGCTGACACACAAGTGCGTTGTGAGCGCAGTA
  GACGTCACCACCGGCGCGGATCCCCGGAGGAGGCCACTGAACGGGAACCTCGGCGCCACCGTGCCCACCG
  GCACGCACAGGACTCAAGCAAGGAAGGCAAGGAGGGCACTGCACCGGTGCTTGTACCCAAGGGCGAGCGT
  CGCGCAAGACATCGAGGCCCGCGTACGGGCCCCCGTGAGACAGAGAACAGTGAGGAGCCCACACGCAGGC
  ACCGTGCAAAGCATAAGGTGCCACCAACACTTGAGCCCCCAGAGAGGGAGGTTGCAGAGAAGGAGAGCAA
  CGTGGTGGAAGGGGATAAGGAAACTCGAAATCACCAGCCCAAGGAACCTCGCTGTGACCTGGAGGCCATT
  GCGGTTACAGGCGTGGGCTCTCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAACAGCCAG
  AGGATGCAGACAACCAGCGTAATGTCACCCGGATGGGCAGTCAGCCCTCAGACCCCAGCACCACTGTGCA
  TGTCCCAGTGACACTGACAGGCCCTCCCGGGGAGGCCACTGTAGTTCCCAGTGCTAACACGGACCTGGAA
  GGCCAAGCGGAGGGCAAGAAGGAGGCAGAGGCTGACGATGTGCTGAGAAGAGGCCCCAGGCCCATCGTTC
  CCTACAGTTCCATGTTCTGCCTCAGCCCCACCAACCTACTCCGTCGCTTCTGCCATTACATTGTGACCAT
  GCGGTACTTTGAGATGGTGATTCTTGTGGTCATCGCCTTGAGCAGCATTGCCCTGGCTGCTGAGGATCCC
  GTGCGGACCGACTCATTCCGGAACAATGCTCTGAAGTACATGGACTACATCTTTACAGGAGTCTTCACCT
  TTGAGATGGTCATAAAGATGATAGACTTGGGCCTGCTGCTGCACCCTGGGGCCTACTTCCGGGACCTGTG
  GAACATTCTGGACTTCATTGTTGTCAGTGGAGCCCTGGTGGCATTTGCATTCTCAGGATCCAAAGGGAAA
  GACATCAATACCATCAAGTCTCTGAGAGTCCTGCGAGTCCTGCGGCCCCTCAAGACCATCAAGCGGCTGC
  CTAAACTCAAGGCTGTGTTTGACTGTGTGGTGAACTCTCTGAAGAATGTCTTGAACATCCTGATCGTCTA
  CATGCTCTTCATGTTTATATTTGCCGTCATCGCCGTCCAACTCTTCAAAGGGAAGTTCTTTTACTGCACT
  GATGAGTCCAAGGAGCTGGAGCGGGACTGCAGGGGTCAGTATTTGGATTATGAGAAGGAAGAGGTAGAAG
  CCCAGCCAAGGCAGTGGAAGAAATATGACTTCCACTATGACAATGTGCTCTGGGCCTTGCTGACTCTGTT
  TACGGTGTCCACAGGAGAGGGGTGGCCCATGGTGCTGAAACACTCTGTGGACGCCACCTATGAGGAGCAG
  GGGCCAAGCCCCGGGTTTCGGATGGAGCTTTCCATCTTCTATGTGGTCTACTTTGTGGTCTTCCCTTTTT
  TCTTTGTCAACATCTTTGTGGCCTTGATCATCATCACCTTCCAGGAGCAGGGGGACAAGGTGATGTCTGA
  GTGCAGTCTGGAAAAGAATGAGAGGGCTTGCATTGACTTTGCCATCAGCGCCAAACCCCTGACACGGTAC
  ATGCCTCAGAACAAGCAGTCGTTCCAGTATAAGACATGGACATTTGTGGTCTCTCCACCCTTTGAGTACT
  TCATTATGGCCATGATAGCCCTCAACACAGTGGTGCTGATGATGAAGTTCTACGATGCCCCTTATGAGTA
  CGAGCTGATGCTGAAGTGCTTGAACATCGTCTTCACATCCATGTTCTCTCTGGAGTGCATCCTGAAGATC
  ATCGCCTTCGGGGTGTTGAACTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACTGTTTTGGGAA
  GTATTACTGATATTTTAGTAACGGAGATTGCGGAAACGAACAACTTCATCAACTTGAGCTTCCTTCGCCT
  CTTCCGGGCAGCACGGCTGATCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCTTGTTATGGACCTTT
  GTCCAGTCCTTTAAGGCGCTGCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTACGCCATCA
  TCGGCATGCAGGTTTTTGGAAACATTGCCCTTGATGATGGCACCAGCATCAACCGACACAACAACTTCCG
  GACATTTCTGCAAGCCTTAATGCTGTTGTTCAGGAGTGCCACTGGGGAGGCCTGGCACGAAATCATGCTG
  TCTTGCCTGGGCAACCGGGCCTGCGACCCACATGCCAACGCCAGCGAATGCGGGAGCGACTTTGCCTATT
  TTTATTTTGTCTCCTTCATCTTCCTCTGTTCCTTTCTGATGCTGAACCTCTTTGTTGCTGTGATCATGGA
  CAATTTCGAATACCTCACGCGGGATTCTTCCATCCTAGGGCCGCACCACCTCGATGAATTCATTCGCGTC
  TGGGCTGAATACGACCCAGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTGAAACACA
  TGTCCCCACCTCTGGGTTTGGGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTCGAATGAA
  CATGCCCATATCCAATGAGGACATGACGGTACACTTTACATCCACACTGATGGCCCTCATCCGGACGGCA
  CTGGAGATCAAGCTTGCCCCAGCGGGGACAAAACAGCACCAATGTGATGCTGAGCTGAGGAAGGAGATCT
  CTTCTGTGTGGGCTAATCTGCCCCAGAAGACTCTGGACTTACTGGTGCCACCCCACAAACCTGACGAGAT
  GACAGTGGGGAAGGTCTATGCGGCTCTCATGATATTTGACTTCTACAAACAGAACAAAACCACCAGAGAT
  CAGACTCACCAAGCTCCTGGAGGCCTGTCCCAGATGGGTCCTGTTTCCCTGTTCCATCCTCTGAAGGCCA
  CCCTGGAGCAGACACAGCCCGCTGTGCTCCGAGGAGCTCGGGTTTTCCTTCGACAAAAGAGTGCAACTTC
  CCTCAGCAATGGGGGCGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCCCTGTCCTGGGGCACGCAG
  AGGACCCAGGACGTACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAGAGATCCCTGTGG
  GGCAGCCAGGAGCACTGGCTGTAGATGTCCAGATGCAGAACATGACATTGAGAGGACCGGATGGGGAGCC
  CCAGCCTGGCCTGGAGAGCCAAGGCCGAGCGGCCTCTATGCCACGCCTGGCGGCAGAAACACAGCCGGCC
  CCTAATGCCAGCCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCGCATGGGACTCAGCTTTGCA
  ACACAGTCCTGGACCGGCCACCTCCTAGCCAGGTGTCCCATCACCACCACCACCGCTGCCACCGGCGCAG
  GGACAAGAAGCAGAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACACAGAAGGTGCACCAAGTACT
  GCTGCAGGATCTGGCCTGCCCCATGGAGAAGGGTCCACAGGCTGCCGGCGGGAGCGTAAGCAAGAGCGAG
  GCCGGTCCCAGGAGCGGAGGCAGCCCTCCTCCTCTTCTTCAGAGAAGCAGCGCTTCTATTCCTGTGACCG
  CTTTGGGAGCCGGGAGCCCCCACAACCTAAGCCCTCCCTCAGTAGCCACCCCATATCGCCAACAGCGGCA
  CTAGAGCCAGGACCCCACCCGCAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGTCAACATCTGGTG
  CTAGCACGCCGGGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCCCTGACCCCACGCCCCAGCATCAC
  CTACAAGACGGCCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGTGGCCTTCCAGCCTTCTCCCCT
  GGCCGTCTCAGCCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGAGCCAGCCTCTAG
  CTTCTGGCTCCCGCATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGCCTCTGCCCACAA
  CCTGCCTGAGGATACACTCACCTTTGAAGAGGCCGTGGCCACCAACTCTGGCCGCTCCTCCAGGACTTCC
  TATGTGTCCTCCCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTACCACTGCACTTTGG
  GACTCAGCACCGGCGTCCGGGCGCGGCACAGCTACCACCACCCAGACCAGGATCACTGGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217766 representing NM_001195199
  Red=Cloning site Green=Tags(s)
  
  MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFT
  VNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGI
  FCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQ
  VVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCDSD
  TECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNL
  VLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRA
  ATKKSRNDLIHAEEGEDRFVDLCAAGSPFARASLKSGKTESSSYFRRKEKMFRFLIRRMVKAQSFYWVVL
  CVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIF
  EVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQ
  LFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYT
  LLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSS
  SVSSVNSPQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVV
  EPGRDGLRGPAGNKSKPEGTEATEGADPPRRHHRHRDRDKTSASTPAGGEQDRTDCPKAESTETGAREER
  ARPRRSHSKEAPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGKEGTAPVLVPKGER
  RARHRGPRTGPRETENSEEPTRRHRAKHKVPPTLEPPEREVAEKESNVVEGDKETRNHQPKEPRCDLEAI
  AVTGVGSLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGEATVVPSANTDLE
  GQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDP
  VRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGK
  DINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCT
  DESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQ
  GPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRY
  MPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSLECILKI
  IAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTF
  VQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDGTSINRHNNFRTFLQALMLLFRSATGEAWHEIML
  SCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRV
  WAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTA
  LEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRD
  QTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQ
  RTQDVLYEARAPLERGHSAEIPVGQPGALAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPA
  PNASPMKRSISTLAPRPHGTQLCNTVLDRPPPSQVSHHHHHRCHRRRDKKQRSLEKGPSLSVDTEGAPST
  AAGSGLPHGEGSTGCRRERKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQPKPSLSSHPISPTAA
  LEPGPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSP
  GRLSRGLSEHNALLQKEPLSQPLASGSRIGSDPYLGQRLDSEASAHNLPEDTLTFEEAVATNSGRSSRTS
  YVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195199
• ORF Size: 7062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001195199.1, NP_001182128.1
• RefSeq Size: 7065
• RefSeq ORF: 7065
• Locus ID: 257648
• MW: 264 kDa