Lrrk2 (NM_001191789) Rat Tagged ORF Clone

CAT#: RR217777

Lrrk2 (myc-DDK-tagged) - Rat leucine-rich repeat kinase 2 (Lrrk2)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217777 representing NM_001191789
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTGGCGCCTGTCAGGGCTGCGACGAGGAAGAGGAGGAGGAGGCTCTGAAGAAGTTGATAGTCA
  GGCTGAATAATGTCCAGGAAGGCAAGCAGATCGAGACGTTGCTCCAGCTCCTGGAGGACATTCTGGTGTT
  CACCTACTCCGACCGCGCCTCCAAGTTATTTGAAGGCAAAAATGTCCACGTGCCTCTGTTGATAGTCCTG
  GACTCCTACATGAGAGTCGCCAGTGTGCAGCAGGTGGGGTGGTCACTTCTGTGCAAATTAATAGAAGTCT
  GTCCAGGGACATTGCAAAGCTTAATAGGACCCCAGGATATTGGGAATGATTGGGAAGTCCTTGGTATTCA
  CCGACTGATTCTTAAAATGTTAACTGTTCATCATGCCAACGTAAACCTGTCAATAGTTGGACTAAAAGCC
  TTAGATCTCCTCCTAGATTCAGGTAAAATTACTCTGCTGATACTGGATGAAGAATGTGATGTTTTCCTGT
  TAATTTTTGATGCCATGCACAGATATTCAGCCAACGAGGAAGTCCAGAAGCTTGCGTGCAAGGCTTTACA
  TGTGCTGTTCGAGAGAGTGTCCGAGGAGCAACTGACTGAGTTTGTGGAGAACAAAGATTACATGACCCTG
  CTGAGTACGTTCCGCAGCTTCAAGAGGGACGAGGAGATTGTGCACCATGTACTCTGCTGCCTGCATTCTC
  TGGCCGTCACTTGCAGCAATGTGGAGGTCCTCATGAGTGGGAATGTCAGGTGTTACAATATTGTGGTGGA
  AGCCATGAAAACATTCCCCACCAGTGAAAACATTCAAGAGGTGAGCTGCTCCTTGCTCCACAAGCTTACA
  TTAGGTAATTTTTTCAACATCCTGGTGTTGAACGAAGTCCATGTCTTTGTGGTGAAAGCCGTCCAGCGGT
  ATCCCGAGAACGTAGCCTTACAGATCTCTGCACTCAGCTGCTTAGCCCTCCTCACCGAGACTATTTTCTT
  AAACCAAGACCTGGAAGAAAGAAGTGAGACTCAGGAAAACAGCGATGAGGACAGTGAGAAGCCTTTCTGG
  TTGGAACCCTGCTATAAAGCCCTGATGCGCCATCGAAAGAACAAACACGTGCAGGAGGCCGCCTGCTGGG
  CCCTAAATAATCTCCTCATGTACCAGAGCAGTTTGCACGAGAAGATTGGAGATGAAGATGGCCAGTTCCC
  GGCGCACAGGGAAGTGATGCTGTCTATGCTGATGCACTCTTCTTCCAAAGACGTCTTCCAAGCAGCTGCG
  CATGCTCTGTCCACTCTCTTGGAACAAAACGTTAATTTCAGGAAAATCCTGCTTGCAAAAGGAGTGTACC
  TGAATGTCTTGGAGTTGATGCAGCGGCACGCCCAGGTTCCTGAGGTGGCAGAGAGTGGCTGCAAGATGCT
  GAGTCATCTGTTTGAAGGAAGCAACCCTTCTTTGGATACAGTGGCGGCAGTGATCCCCAAAATACTAACA
  GTGATGAGAACCCATGGAACGTCTCTGTCAGTCCAGCTGGAGGCACTGCGAGCTCTTCTGCATTTTGTGG
  TGCCGGGAGTATCAGAAGATTCCAGGGATGACTCGCGATGCCAACCAAACGTGCTCAGAACACAGTGCTT
  CAGGACTGACATCCACAAGCTGGTTCTAGCCGCTCTGAACAGGTTCATTGGGAATCCCGGGATTCAGAAA
  TGTGGATTGAAAGTCATCTCTTCTTTCGCACATCTTCCCGATGCCTTAGAGATGTTATCCCTGCATGGAG
  CAGTTGACTCAGTCCTCCATACCTTACAGATGTATCCAGATGACCAAGAAATTCAGTGTCTGGGCTTACA
  CCTTATGGGATGCCTGATGACAAAGAAGAATTTCTGCATAGGGACAGGGCACCTCCTGGCAAAAATTCTG
  GCTTCCACCTTGCAGCGATTTAAAGATGTTGCTGAAGTACAGACTACAGGATTACAGACGGTCTTGTCAA
  TGCTTGACCTGTCCGTATCTTTCTCCAAGCTGCTAGTGCACTATTCATTTGATGTGGTGATGTTTCATCA
  GATGTCTTCCGGTGTCCTGGAACAAAAGGATGAGCAGTTTCTCAACTTATGCTGCAAATGCTTTGCAAAA
  GTGGCTGTGGATGATGAGCTGAAAAGCAAGATGCTAGAGAGAGCCTGCGATCAGAACAACAGCATCATGG
  TCGAATGTTTGCTCCTCTTGGGAGCCGATGCCAATCAAGCGAAGGGGGCAACTTCTTTAATCTATCAGGT
  ATGTGAGAAAGAGAGCAGCCCTAAATTGGTGGAACTATTGCTTAACAGTGGGTGCCGTGAACAAGATGTA
  CGGAAAGCCCTGACAGTAAGCATCCAAAAGGGCGACAACCAGGTCATCAGCTTACTCCTGAGGAGACTTG
  CCCTGGACCTGGCCAACAACAGCATTTGCCTTGGAGGATTTTGCATAGGAAAACTTGATCCTTCTTGGCT
  AGGCCCTTTATTTCCAGATAAGTCATCTAATTTGAGGAAACAAACAAATGCGGGGTCTGTCCTAGCGAGG
  AAAGTGCTCCGGTATCAGATGAGAAACACTCTTCAAGAAGGCGTGGCCTCAGGCAGTGAGGGCAACTTCT
  CTGAGGATGCGCTGGCGAAATTTGGCGAATGGACCTTCATTCCCGACTCTTCTATGGACAGTGTGTTTGG
  CCAGAGTGACGATCTGGATAGCGAAGGCAGCGAGAGCTCCTTTCTGGTGAAGAAGAAGTCCAACTCAGTT
  AGTGTAGGAGAAGTTTACAGGGACCTAGCTCTGCAGCGCTGCTCACCAAATGCTCAGAGGCACTCCAGTT
  CCTTGGGTCCTGTTTTTGATCACGAAGATCTACTGAGACGAAAAAGAAAAATACTGTCCTCAGATGAGTC
  TCTCAGATCCTCAAGGCTGCAGTCCCATACGAGACAATCAGATAGCTCTTCTTCTCTGGCTTCTGAGAGA
  GAACACATCACGTCTTTAGACCTTTCTGCCAACGAACTGAAAGATATTGATGCTCTGGGCCAGAAGTGTT
  GCCTCAGTAGCCACCTGGAGCATCTCACCAAGCTGGAACTTCACCAGAATTCACTCACGAGCTTCCCACA
  ACAGCTGTGTGAGACTCTGAAGTGCTTGACACATCTGGATTTGCACAGTAACAAATTCGCCACCTTTCCC
  TCCTTCATGTTGAAAATGCCAAGTGTTATCCACCTAGACGCCTCTCGAAATGACATCGGACCAACAGTTG
  TTTTAGACCCTGTGGTGAAGTGTCCAAGCCTCAAACAGTTTAACCTGTCCTACAACCAGCTCTCTTCCAT
  CCCAGAGAACCTGGACCAAGTGGTGGAGAAACTGGAGCAGCTCCTACTGGAAGGAAACAAAATATCCGGG
  ATTTGTTCTCCCTTGAGCCTGAAGGAACTGAAGATTTTAAACCTTAGTAAAAACCACATTCCATCCCTAC
  CTGAAGACTTTCTCGAGGCTTGCCCGAAAGTGGAGAGCTTCAGTGCCCGCATGAATTTTCTCGCTGCAAT
  GCCTGCCTTACCGTCTTCCATAACTAGCTTAAAATTGTCTCAAAACTCTTTCACGTGCATTCCAGAAGCG
  ATCTTCAGTCTTCCACACTTGCGGTCCTTGGATATGAGTCACAACAACATTGAACACCTGCCGGGACCTG
  CACATTGGAAGTCTCTGAACTTAAGGGAACTCATTTTTAGCAAGAATCAGATCAGCACCTTAGACTTGAG
  CGAAAACCCACACATATGGTCAAGAGTAGAGAAGCTGCATCTCTCTCATAATAAACTGAAAGAGATTCCT
  CCAGAAATTGGCCGTCTTGAAAACCTGACATCTCTTGATGTCAGTTACAACCTGGAACTGAGGTCCTTTC
  CAAACGAAATGGGGAAGTTAAGCAAAATATGGGATCTTCCCTTGGATGGACTGCACCTCAACTTTGACTT
  TAAGCACATAGGATGCAAAGCCAAAGACATCATAAGGTTTCTACAACAACGTCTGAAAAAGGCCGTGCCC
  TACAACCGAATGAAGCTCATGATTGTGGGCAATACGGGGAGTGGTAAGACCACTCTACTGCAGCAGCTCA
  TGAAAATGAAGAAATCAGAACTCGGCATGCAGGGCGCCACGGTTGGCATAGACGTGCGAGACTGGCCCAT
  CCAAATACGAGGCAAAAGGAAAAAGGACCTTGTTCTAAACGTGTGGGACTTTGCAGGCCGTGAGGAATTC
  TACAGCACTCACCCCCACTTCATGACCCAGAGAGCCCTGTACCTGGCTGTCTACGACCTCAGCAAGGGGC
  AGGCGGAGGTGGATGCCATGAAGCCCTGGCTCTTCAACATCAAGGCTCGTGCCTCTTCTTCCCCGGTGAT
  TCTGGTGGGCACACATTTGGATGTTTCTGATGAGAAGCAGCGCAAAGCCTGCATAGGCAAAATCACGAAG
  GAACTCCTTAATAAGCGAGGATTCCCCACCATCCGGGACTACCACTTCGTGAATGCCACTGAGGAGTCGG
  ATGCGCTGGCAAAGCTCCGGAAAACCATCATAAATGAGAGTCTTAATTTCAAGATCCGAGATCAGCCCGT
  GGTTGGGCAGCTAATTCCAGATTGCTACGTAGAACTGGAGAAAATAATCTTATCGGAGCGTAAAGCTGTA
  CCAACGGAGTTTCCTGTAATTAACCGGAAACACTTACTCCAGCTGGTGAAGGAACACCAGCTGCAGCTGG
  ATGAGAACGAGCTCCCCCACGCTGTTCACTTCCTGAATGAGTCAGGAGTTCTTCTGCATTTTCAAGACCC
  CGCATTGCAGCTGAGTGACCTGTACTTTGTGGAACCCAAGTGGCTTTGTAAAGTCATGGCACAGATTTTG
  ACCGTGAAAGTGGACGGCTGCCTGAAGCATCCTAAGGGCATCATTTCACGGAGAGATGTGGAAAAATTCC
  TTTCCAAGAAAAAGCGATTCCCTAAGAACTACATGGCGCAGTACTTCAAACTTTTAGAAAAATTTCAGAT
  CGCATTACCAATAGGGGAAGAATATCTGCTGGTTCCAAGCAGCTTATCTGACCACAGGCCAGTGATAGAG
  CTCCCCCACTGTGAGAACTCTGAGATCATCATCCGGCTGTATGAAATGCCATACTTTCCAATGGGATTTT
  GGTCAAGATTGATTAACCGATTACTTGAAATCTCACCTTTCATGCTTTCTGGAAGAGAGAGAGCACTACG
  CCCAAACCGAATGTACTGGCGCCAAGGCATCTACTTGAATTGGTCTCCAGAAGCCTACTGTCTGGTGGGC
  TCTGAAGTCTTAGACAGTCGCCCAGAGAGTTTCTTGAAAATCACAGTTCCATCTTGTAGAAAAGGTTGTA
  TTCTTTTGGGCCGAGTTGTGGATCATATTGACTCACTCATGGAAGAATGGTTTCCTGGATTGCTGGAGAT
  TGACATTTGTGGGGAAGGAGAAACTTTGTTGAAAAAATGGGCATTGTATAGTTTTAATGATGGCGAAGAA
  CATCAGAAGATCTTGCTTGATGAGTTGATGAAGAAGGCTGAAGAAGGAGACCTGTTAATAAATCCAGATC
  AACCAAGGCTCACCATTCCAATATCCCAGATTGCTCCGGACTTGATCTTGGCTGACCTGCCTAGAAATAT
  TATGTTGAACAATGACGAACTGGAATTTGAGGAAGCACCCGAGTTTCTCTTAGGTGATGGAAGTTTCGGA
  TCAGTTTATCGAGCTGCCTACGAAGGAGAGGAAGTGGCTGTGAAGATTTTTAATAAGCACACATCGCTTA
  GGCTGTTAAGACAAGAGTTGGTGGTACTCTGTCATCTCCACCATCCCAGCTTGATCTCCCTGTTGGCGGC
  TGGGATTCGTCCTCGGATGCTGGTAATGGAGTTGGCCTCCAAGGGTTCCTTGGATCGCCTGCTGCAGCAG
  GACAAAGCCAGCCTCACCCGGACCCTCCAGCACAGAATCGCATTGCATGTGGCCGATGGCCTGAGATATC
  TGCACTCGGCCATGATTATTTACCGTGATCTGAAGCCCCACAACGTGCTACTCTTCACCCTGTATCCCAA
  TGCCGCCATCATTGCGAAGATTGCGGACTACGGGATTGCACAGTACTGCTGTAGGATGGGAATAAAGACC
  TCAGAGGGCACCCCAGGGTTCCGAGCACCTGAAGTTGCCAGAGGAAATGTCATTTATAACCAACAGGCTG
  ATGTTTATTCTTTTGGCTTACTACTTCATGATATCTGGACAACTGGGAATAGAATCATGGAGGGTTTGAG
  GTTTCCAAATGAGTTTGATGAACTGGCCATACAAGGGAAATTGCCAGACCCAGTTAAAGAATATGGCTGT
  GCCCCGTGGCCTATGGTTGAGAAGTTAATTACAAAATGTTTGAAAGAAAATCCTCAAGAAAGACCCACTT
  CTGCCCAGGTCTTTGACATTTTGAATTCAGCTGAGTTAATTTGCCTCATGCGACACATTTTCATACCTAA
  GGACATCACTGTTGAATGCATAGCTGCTACAAACCTCAATAGCAAGCGAGCGACTCTCTGGTTGGGCTGT
  GGGAACACAGAAAAAGGGCAGCTTTCCTTACTTGACTTGAACACGGAAAGATACAGCTATGAGGAAGTTA
  CTGATAGTAGAATACTGTGCCTGGCTTTGGTGCATCTTGCTGCTGAGAAAGAGAGCTGGGTTGTGTGTGG
  GACACAGTCCGGAGCTCTCCTGGTCATCAATGCTGAAGATGAGACAAGGAGACACACCCTCGACAAGATG
  ACTGATTCTGTTACTTGCTTGTATTGCAATTCCTTTGCCAAGCAGAGCAAGCAAAGTCACTTCCTTTTGG
  TGGGAACTGCTGATGGCAACTTAATGATATTTGAAGATAAGACCATTAAGTGTAAAGGAGCTGCCCCATT
  GAAGACACTACACATAGGCGATGTCAGTACGCCCCTGATGTGCCTGAGCGAGTCCATGAATTCATCTGAA
  AGACACATCACATGGGGAGGGTGTGGCACAAAGATCTTCTCCTTTTCCAATGATTTCACCATTCAGAAAC
  TCATCGAGACAAGAACCAACCAGCTGTTTTCTTACTCAGCGTTCAGCGATTCTAACATCATAGCGGTGGC
  AGTGGACACAGCGCTTTATATTGCCAAGAAAAACAGCCCTGTCGTAGAGGTGTGGGACAAGAAGACAGAA
  AAACTCTGTGAACTAATAGACTGTGTGCACTTCTTAAAGGAGGTGATGGTGAAAATAAACAAGGACTCGA
  AGCACAAGCTGTCCTACTCTGGGAGGGTGAAGGCACTCTGCCTGCAGAAGAACACAGCTCTCTGGATCGG
  AACTGGAGGAGGCCACATCTTACTCCTGGATCTTTCTACACGGCGAGTCATCCGCACCATCCACAATTTC
  TGTGATTCCGTGAGAGCCATGGCCACAGCTCAGTTAGGCAACCTTAAAAATGTCATGCTGGTTTTGGGGT
  ACAAGCGGAAGAGTACAGAAGGAACCCAAGAACAAAAAGAGATACAATCTTGTTTGTCTATTTGGGACCT
  CAATCTTCCACATGAAGTGCAAAACTTAGAAAAACACATTGAAGTAAGAACAGAACTGGCTGATAAAATG
  AGGAAAACATCTGTCGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217777 representing NM_001191789
  Red=Cloning site Green=Tags(s)
  
  MASGACQGCDEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDILVFTYSDRASKLFEGKNVHVPLLIVL
  DSYMRVASVQQVGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKA
  LDLLLDSGKITLLILDEECDVFLLIFDAMHRYSANEEVQKLACKALHVLFERVSEEQLTEFVENKDYMTL
  LSTFRSFKRDEEIVHHVLCCLHSLAVTCSNVEVLMSGNVRCYNIVVEAMKTFPTSENIQEVSCSLLHKLT
  LGNFFNILVLNEVHVFVVKAVQRYPENVALQISALSCLALLTETIFLNQDLEERSETQENSDEDSEKPFW
  LEPCYKALMRHRKNKHVQEAACWALNNLLMYQSSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAA
  HALSTLLEQNVNFRKILLAKGVYLNVLELMQRHAQVPEVAESGCKMLSHLFEGSNPSLDTVAAVIPKILT
  VMRTHGTSLSVQLEALRALLHFVVPGVSEDSRDDSRCQPNVLRTQCFRTDIHKLVLAALNRFIGNPGIQK
  CGLKVISSFAHLPDALEMLSLHGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKIL
  ASTLQRFKDVAEVQTTGLQTVLSMLDLSVSFSKLLVHYSFDVVMFHQMSSGVLEQKDEQFLNLCCKCFAK
  VAVDDELKSKMLERACDQNNSIMVECLLLLGADANQAKGATSLIYQVCEKESSPKLVELLLNSGCREQDV
  RKALTVSIQKGDNQVISLLLRRLALDLANNSICLGGFCIGKLDPSWLGPLFPDKSSNLRKQTNAGSVLAR
  KVLRYQMRNTLQEGVASGSEGNFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKKKSNSV
  SVGEVYRDLALQRCSPNAQRHSSSLGPVFDHEDLLRRKRKILSSDESLRSSRLQSHTRQSDSSSSLASER
  EHITSLDLSANELKDIDALGQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLTHLDLHSNKFATFP
  SFMLKMPSVIHLDASRNDIGPTVVLDPVVKCPSLKQFNLSYNQLSSIPENLDQVVEKLEQLLLEGNKISG
  ICSPLSLKELKILNLSKNHIPSLPEDFLEACPKVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEA
  IFSLPHLRSLDMSHNNIEHLPGPAHWKSLNLRELIFSKNQISTLDLSENPHIWSRVEKLHLSHNKLKEIP
  PEIGRLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHIGCKAKDIIRFLQQRLKKAVP
  YNRMKLMIVGNTGSGKTTLLQQLMKMKKSELGMQGATVGIDVRDWPIQIRGKRKKDLVLNVWDFAGREEF
  YSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACIGKITK
  ELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKAV
  PTEFPVINRKHLLQLVKEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQIL
  TVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMAQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIE
  LPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVG
  SEVLDSRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEE
  HQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSFG
  SVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQ
  DKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKT
  SEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGNRIMEGLRFPNEFDELAIQGKLPDPVKEYGC
  APWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHIFIPKDITVECIAATNLNSKRATLWLGC
  GNTEKGQLSLLDLNTERYSYEEVTDSRILCLALVHLAAEKESWVVCGTQSGALLVINAEDETRRHTLDKM
  TDSVTCLYCNSFAKQSKQSHFLLVGTADGNLMIFEDKTIKCKGAAPLKTLHIGDVSTPLMCLSESMNSSE
  RHITWGGCGTKIFSFSNDFTIQKLIETRTNQLFSYSAFSDSNIIAVAVDTALYIAKKNSPVVEVWDKKTE
  KLCELIDCVHFLKEVMVKINKDSKHKLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNF
  CDSVRAMATAQLGNLKNVMLVLGYKRKSTEGTQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKM
  RKTSVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191789
• ORF Size: 7578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001191789.1, NP_001178718.1
• RefSeq Size: 7581
• RefSeq ORF: 7581
• Locus ID: 300160
• MW: 284.9 kDa