LYK5 (STRADA) (NM_153335) Human Untagged Clone

CAT#: SC100826

STRADA (untagged)-Human STE20-related kinase adaptor alpha (STRADA), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STRADA
• Synonyms: LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD alpha
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100826 sequence for NM_153335 edited (data generated by NextGen Sequencing)
  ATGTCATTTCTTACCAATGATGCGAGCTCAGAGTCAATAGCATCCTTCTCTAAACAGGAG
  GTCATGAGTAGCTTTCTGCCAGAGGGAGGGTGTTACGAGCTGCTCACTGTGATAGGCAAA
  GGATTTGAGGACCTGATGACTGTGAATCTAGCAAGGTACAAACCAACAGGAGAGTACGTG
  ACTGTACGGAGGATTAACCTAGAAGCTTGTTCCAATGAGATGGTAACATTCTTGCAGGGC
  GAGCTGCATGTCTCCAAACTCTTCAACCATCCCAATATCGTGCCATATCGAGCCACTTTT
  ATTGCAGACAATGAGCTGTGGGTTGTCACATCATTCATGGCATACGGTTCTGCAAAAGAT
  CTCATCTGTACACACTTCATGGATGGCATGAATGAGCTGGCGATTGCTTACATCCTGCAG
  GGGGTGCTGAAGGCCCTCGACTACATCCACCACATGGGATATGTACACAGGAGTGTCAAA
  GCCAGCCACATCCTGATCTCTGTGGATGGGAAGGTCTACCTGTCTGGTTTGCGCAGCAAC
  CTCAGCATGATAAGCCATGGGCAGCGGCAGCGAGTGGTCCACGATTTTCCCAAGTACAGT
  GTCAAGGTTCTGCCGTGGCTCAGCCCCGAGGTCCTCCAGCAGAATCTCCAGGGTTATGAT
  GCCAAGTCTGACATCTACAGTGTGGGAATCACAGCCTGTGAACTGGCCAACGGCCATGTC
  CCCTTTAAGGATATGCCTGCCACCCAGATGCTGCTAGAGAAACTGAACGGCACAGTGCCC
  TGCCTGTTGGATACCAGCACCATCCCCGCTGAGGAGCTGACCATGAGCCCTTCGCGCTCA
  GTGGCCAACTCTGGCCTGAGTGACAGCCTGACCACCAGCACCCCCCGGCCCTCCAACGGT
  GACTCGCCCTCCCACCCCTACCACCGAACCTTCTCCCCCCACTTCCACCACTTTGTGGAG
  CAGTGCCTTCAGCGCAACCCGGATGCCAGGTATCCCTGCTGGCCTGGGCCTGGGCTTCGG
  GAGAGCAGAGGGTGCTCAGGAGGGTAA
  
  Clone variation with respect to NM_153335.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153335 unedited
  AAACGCAATTCGCACGAGGCTCCCCTCCCAGCAACCGGTCTGGCGGCGGCGCGGCAGTAA
  AACTGAGGAGGCGGAGCCAAGACGGTCGGGGCTGCTTGCTAACTCCAGGAACAGGTTTAA
  GTTTTTGAAACTGAAGTAGGCCTACACAGTAGGAACTCATGTCATTTCTTGTAAGTAAAC
  CTTAGCGAATCAGGACCAATGATGCGAGCTCAGAGTCAATAGCATCCTTCTCTAAACAGG
  AGGTCATGAGTAGCTTTCTGCCAGAGGGAGGGTGTTACGAGCTGCTCACTGTGATAGGCA
  AAGGATTTGAGGACCTGATGACTGTGAATCTAGCAAGGTACAAACCAACAGGAGAGTACG
  TGACTGTACGGAGGATTAACCTAGAAGCTTGTTCCAATGAGATGGTAACATTCTTGCAGG
  GCGAGCTGCATGTCTCCAAACTCTTCAACCATCCCAATATCGTGCCATATCGAGCCACTT
  TTATTGCAGACAATGAGCTGTGGGTTGTCACATCATTCATGGCATACGGTTCTGCAAAAG
  ATCTCATCTGTACACACTTCATGGATGGCATGAATGAGCTGGCGATTGCTTACATCCTGC
  AGGGGGTGCTGAAGGCCCTCGACTACATCCACCACATGGGATATGTACACAGGAGTGTCA
  AAGCCAGCCACATCCTGATCTCTGTGGATGGGAAGGTCTACCTGTCTGGTTTGCGCAGCA
  ACCTCAGCATGATAAGCCATGGGCAGCGGCAGCGAGTGGTCCACGATTTTCCCAAGTACA
  GTGTCAAGGTTCTGCCGTGGCTCAGCCCCGAGTCCTCCAGCAGAATCTNCAGGGTTATGA
  TGCCAAGTCTGACAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153335 unedited
  CGACGAAAATTCGGCGTTCTCTGANAAATATTTAATTTGTTNCTGNACAACGNAAACTAA
  CACAGCATAAGGACTTAAGCCCTCAAGCTAGGCAAGACACTGATGGCCCTTGGAGAAAGC
  ACCAGGGCAGCTGGCAGGAGGCGCTAAGATGGATTCTTGGTGGGAAAGGAATGAGGCACA
  CACATAAACCGTGGGAAAGACTTAGGTGTCAGGGCCCAAGCCAGAAGAGGGATAAGGATG
  GACTGTTCCAGCCTTAGTCTGTGCTTAAAAAGACAGAAATGCCCTTCTCTGGAATTATGA
  GGGAGAGGTCTGAGGGAAGGGAGGCAGGCAGCTTACTACTTGCCCTTTCACTGACCTATA
  GCATCTGAGGCATCTGAGAGCAAAGTTAAATAGAATGAGGCTGGTCGCAAGCACTGGGAA
  GAGGTGGCGGTCCTCGGGTTTAAGGAGCCAAACCCTGGGCTTGGAATTCAGCTCCCAGGG
  CCAGAGCAGCATCTCTCTTCTGCGGAGGAGGTCACCTGAGCTCACAGGACTGGGAATGTC
  GGCTTTGGACCCTCCTGATCCCTGGTTGTTGAGATTCCCTTGTGTCTCANAAGCCTTGGA
  GCAGTGAAGTGTCTCTCCAGGATTTTCTTTCCCAATCAGTCAGCAGTCAACTTTCCTGGA
  AGATGTCCTTTCTACCCAATCTGCCCAGGAGGGCGGGAATGTGGCCGGCCCTCAGAAGGG
  CCTCTGGGTGGCCTCTGCATCCCTGGCTGGAGATGCGCACAGTTTGCAGAGGCTCAGAAC
  TCCCAATCGTCCAACTCCAGCTCTTCCAGGTTTGTTACCAGGCCAAAGATTCACTGTGGT
  CCTGAGACTGGCTGCCCTCAAAATGGTGATGGGGGTGACAGGACGAAGCAATTCGGCCAA
  ACCTCTGAGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153335
• ORF Size: 1047 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_153335.4, NP_699166.2
• RefSeq Size: 2658
• RefSeq ORF: 1047
• Locus ID: 92335
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway
• Gene Summary: The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (3) uses an alternate splice site and lacks two alternate exons in the 5' coding region, and uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (3) lacks an internal segment near the N-terminus and has a shorter and distinct C-terminus, compared to isoform 1.